Applications of Nanoflowers in Biomedicine

Negahdary, Masoud; Heli, H.

doi:10.2174/1872210511666170911153428

Cited by 49 publications

(16 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast to sod-Zn(mIM) 2 , the lamellar nf-Zn(mIM) 2 is nonporous, and has a much higher efficiency for enzyme immobilization. The lamellar nanoflower-like ZIF framework identified in this study complements the wide interest in nanoflower structure in multiple applications such as biomedicine, [47][48][49] and we expect it to be used extensively as a general carrier for enzyme immobilization.…”

Section: Discussionmentioning

confidence: 56%

Lamellar Enzyme-Metal–Organic Framework Composites Enable Catalysis on Large Substrates

et al. 2022

CCS Chem

View full text Add to dashboard Cite

Zeolitic imidazolate framework (ZIF) is a representative type of metal-organic framework that has been extensively used as a carrier in enzyme immobilization. However, thus far the immobilization studies have mainly been carried out on enzymes with small substrates (e.g., peroxide and glucose), and the general applicability of ZIF in enzyme immobilization has not been investigated. In this study, we show that, owing to its small aperture size, the canonical sodalite ZIF [ZIF-8 or sod-Zn(mIM) 2 (mIM = 2-methylimidazolate)] cannot be used for enzymes with large substrates. Instead, we show that the lamellar, nanoflower-like ZIF [nf-Zn(mIM) 2 ] synthesized in low precursor concentrations overcomes the size limitation of sod-Zn(mIM) 2 and is suitable for immobilization of various enzymes with large substrates. Remarkably, the lamellar ZIF can work with a thioesterase, which acts on a peptide with 11 amino acids to produce an analog of teixobactin, a superantibiotic with no antimicrobial resistance thus far. The lamellar ZIF scaffold is highly efficient in enzyme loading and significantly increases enzyme stability against disfavorable conditions, serving as a general carrier in enzyme immobilization.

show abstract

Section: Discussionmentioning

confidence: 56%

Lamellar Enzyme-Metal–Organic Framework Composites Enable Catalysis on Large Substrates

et al. 2022

CCS Chem

View full text Add to dashboard Cite

show abstract

“…Nanostructured materials have been used in many medical applications, for instances, drug delivery, diagnostic and theranostic assays, and treatments [16][17][18][19]. Paper-based microfluidic technologies such as lateral flow test strips (LFTSs) have attracted much attention for rapid diagnostic test applications based on nanomaterials [20].…”

Section: Introductionmentioning

confidence: 99%

Lateral Flow Genochromatographic Strip for Naked-Eye Detection of Mycobacterium Tuberculosis PCR Products with Gold Nanoparticles as a Reporter

Nazari-Vanani

Tondro

et al. 2020

jbpe

View full text Add to dashboard Cite

Background: Mycobacterium tuberculosis (MTB) is a pathogen causing tuberculosis (TB) in human, and TB can cause enormous social and economic disruptions. Lateral flow test strips (LFTSs) are inexpensive, portable, disposable, rapid, and easy-to-use analytical tools.Objective: LFTSs were prepared for the detection of MTB. LFTSs were fabricated using a new specific probe for MTB H37Rv, based on IS6110 sequence gene, and tailed with poly deoxyadenine (dA). Material and Methods:In this experimental study, to create test and control zones, streptavidin (STP) and a 150-mer dA were dotted on a nitrocellolose membrane. Gold nanoparticles (GNPs) were conjugated with poly deoxythymidine sequence and placed on the conjugate pad. The composition of immersion buffers for sample pad and conjugate pad, running solution, solutions of GNPs-S-dT150 and STP were introduced. DNA genome of MTB and Mycobacterium bovis in clinical samples was amplified with PCR, and then detected by the LFTSs. During the assay, samples were firstly hybridized in two steps and then placed on a conjugate pad in a manner that positive and negative samples provided two and one red lines, respectively, on the detection pad.Results: After PCR reaction with biotinylated primer, hybridization process with specific MTB probe-dA70-100 toke 10 min, and running process on the strip was performed within 5 min. Conclusion:We showed that LFTS can discriminate a particular bacteria strain from others. The LFTSs can be redesigned for detection of other pathogenic genomes.

show abstract

“…Nanomaterials are developed due to exhibition of novel characteristics arising from their size-dependent features, and widely used in medical diagnoses and therapies [8][9][10]. These materials have a usage in biological and medical applications such as labeling of biomolecules [11], drug and gene delivery [12][13][14], rapid and sensitive microbial detections [15,16], antimicrobial materials [10,[17][18][19], detection and recognition of DNA and proteins [20][21][22], probing of intrinsic DNA structure [23], biologic substitutes and regenerative medicine [24], hyperthermia [25], separation of specific cell types [26] and MRI contrast agents [27].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Photothermal Treatment of Pseudomonas Aeruginosa by a Carbon Nanoparticles-Polypyrrole Nanocomposite

Behzadpour

2019

J Biomed Phys Eng

View full text Add to dashboard Cite

Background: Nowadays, it is needed to explore new routes to treat infectious bacterial pathogens due to prevalence of antibiotic-resistant bacteria. Antimicrobial photothermal therapy (PTT), as a new strategy, eradicates pathogenic bacteria.Objective: In this study, the antimicrobial effects of a carbon nanoparticles-polypyrrole nanocomposite (C-PPy) upon laser irradiation were investigated to destroy the pathogenic gram-negative Pseudomonas aeruginosa. Material and Methods:In this experimental study, the bacterial cells were incubated with 50, 100 and 250 µg mL -1 concentrations of C-PPy and irradiated with a 808-nm laser at two power densities of 0.5 and 1.0 W cm -2 . CFU numbers were counted for the irradiated cells, and compared to an untreated sample (kept in dark). To explore the antibacterial properties and mechanism(s) of C-PPy, temperature increment, reactive oxygen species formation, and protein and DNA leakages were evaluated. Field emission scanning electron microscopy was also employed to investigate morphological changes in the bacterial cell structures. Results:The results showed that following C-PPy attachment to the bacteria surface, irradiation of near-infrared light resulted in a significant decrement in the bacterial cell viability due to photothermal lysis. Slightly increase in protein leakage and significantly increase intracellular reactive oxygen species (ROS) were observed in the bacteria upon treating with C-PPy. Conclusion:Photo-ablation strategy is a new minimally invasive and inexpensive method without overdose risk manner for combat with bacteria.

show abstract

Applications of Nanoflowers in Biomedicine

Abstract: The use of nanoflowers in biomedicine leads to improving accuracy, reducing time to achieve the results, reducing costs, creating optimal treatment conditions as well as avoiding side effects of the treatment of specific diseases, and increasing functional strength.

Cited by 49 publications

References 76 publications

Lamellar Enzyme-Metal–Organic Framework Composites Enable Catalysis on Large Substrates

Lamellar Enzyme-Metal–Organic Framework Composites Enable Catalysis on Large Substrates

Lateral Flow Genochromatographic Strip for Naked-Eye Detection of Mycobacterium Tuberculosis PCR Products with Gold Nanoparticles as a Reporter

Antimicrobial Photothermal Treatment of Pseudomonas Aeruginosa by a Carbon Nanoparticles-Polypyrrole Nanocomposite

Contact Info

Product

Resources

About