Applications of nanobodies in plant science and biotechnology

Wang, Wenyi; Yuan, Jumao; Jiang, Chenfanfu

doi:10.1007/s11103-020-01082-z

Cited by 27 publications

(24 citation statements)

References 99 publications

(138 reference statements)

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“…The special properties of NPs make them more effective than ordinary materials, e.g., ions and molecules [ 19 ]. In a greenhouse experiment, crops were collected after growing for 80 days in soil modified with CuSO 4 or CuO particles (bulk or nano) [ 20 ].…”

Section: Special Properties and Surface Modifications Of Npsmentioning

confidence: 99%

Phytonanotechnology applications in modern agriculture

et al. 2021

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With the rapidly changing global climate, the agricultural systems are confronted with more unpredictable and harsh environmental conditions than before which lead to compromised food production. Thus, to ensure safer and sustainable crop production, the use of advanced nanotechnological approaches in plants (phytonanotechnology) is of great significance. In this review, we summarize recent advances in phytonanotechnology in agricultural systems that can assist to meet ever-growing demands of food sustainability. The application of phytonanotechnology can change traditional agricultural systems, allowing the target-specific delivery of biomolecules (such as nucleotides and proteins) and cater the organized release of agrochemicals (such as pesticides and fertilizers). An amended comprehension of the communications between crops and nanoparticles (NPs) can improve the production of crops by enhancing tolerance towards environmental stresses and optimizing the utilization of nutrients. Besides, approaches like nanoliposomes, nanoemulsions, edible coatings, and other kinds of NPs offer numerous selections in the postharvest preservation of crops for minimizing food spoilage and thus establishing phtonanotechnology as a sustainable tool to architect modern agricultural practices. Graphical Abstract

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Section: Special Properties and Surface Modifications Of Npsmentioning

confidence: 99%

Phytonanotechnology applications in modern agriculture

et al. 2021

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“…HCAbs present no such drawbacks and can be minimized into a single domain (Nb) that can serve as a stand-alone recognition unit when expressed recombinantly as a single-domain protein, or when part of a fusion [14]. The folding and stability of Nbs is less dependent on the formation of the disulphide bonds, hence they can be produced and function in the reducing cytoplasm of plant cells.…”

Section: Biophysical Properties and Structural Features Of Nanobodiesmentioning

confidence: 99%

“…The latter property is of particular importance when Nbs are applied in establishing resistance against plant pathogens (to reduce crop loss) as plant viruses are generally located within the cytosol [40]. The mechanisms by which Nb mediate resistance in plants include: Nb binding to the key proteins of pathogens preventing their cell attachment, invasion, reproduction and movement, thus interrupting their life cycles, or by directly neutralizing the toxins secreted by pathogens, reducing the damage to plants [14].…”

Section: Engineering Resistant Plants With Nanobodiesmentioning

confidence: 99%

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Nanobodies: their potential for applications in biotechnology, diagnosis and antiviral properties in Africa; focus on application in agriculture

Njeru

Kusolwa

2021

Biotechnology & Biotechnological Equipment

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Antibodies are key elements in routine immunological tests, having a long history in detection of plant pathogens due to their specificity, reliability and fast performance. Heavy chain-only antibodies (HCAbs), circulating in the blood of camels are IgG isotypes that lack the light (L) chains and the heavy (H) chain is depleted of the first constant domain (CH1). The HCAbs interact with the antigen by virtue of only one single variable domain, referred to as VHH or nanobody (Nb). Owing to their unique properties: nanometer scale size, robust structure, stable and soluble behaviour in aqueous solution, high affinity and specificity for only one cognate target, as well as a sustainable source, Nbs are an ideal research tool in plants. There are several rapid and robust techniques for isolating highly specific Nbs. Antigen-specific Nbs are selected from constructed nanobody libraries (immune, naïve, synthetic) and specific binders are retrieved by phage display, yeast display or ribosome display. Their small size, easy folding in monomeric units and the possibility to express Nbs in the cytosol, make them attractive for their application to engineer resistance against plant pathogens, and the effect of multiple Nbs combined by gene stacking to engineer plant resistance can be studied. We summarize the current methodologies for isolation and production of Nbs, their application in detection of plant pathogens and nanobody-mediated resistance against plant pathogens. In addition, we explore novel implementations of VHHs in agricultural research.

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“…More recently, plants are being used in biotechnology as leading expression platforms for antibody production thanks to their efficient post-transcriptional modifications (PTMs) that enable the assembly of complex proteins and the production of diagnostic and therapeutic proteins (Rybicki, 2020). The application of plants as biofactories of recombinant proteins are referred to as "Molecular Farming" (Shanmugaraj et al, 2020;Wang et al, 2021).…”

Section: Plant Biotechnologymentioning

confidence: 99%

Biotechnological Application of Potyvirus-Based Vectors in Cucurbit Plants

Houhou¹

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As a response to viral infections, host plants trigger an RNA-mediated gene silencing defense, to which viruses respond with the expression of viral-encoded RNA silencing suppressors. If virus clones are manipulated to include sequences homologous to host endogenous genes, these are also targeted by the plant RNA silencing machinery. This so-called virus-induced gene silencing (VIGS) has become a powerful technique for reverse genetic analyses in plants, as an alternative to labor-intensive genome transformation. We show that a mild isolate of Watermelon mosaic virus (WMV, genus Potyvirus) can be used as a VIGS vector for reverse genetic analyses in melon. Recombinant WMV clones -in which fragments of the melon Phytoene desaturase (PDS) mRNA were inserted in sense, antisense, and hairpin modalitiesinduced a distinctive phenotype and significant silencing of the endogenous gene. While the foreign fragments in sense and antisense orientations were stable in the viral progeny, the hairpin was quickly lost. Nevertheless, the hairpin construct triggered a maintained silencing effect comparable to those of the sense and antisense constructs. The suitability of WMV as a VIGS vector was further confirmed targeting melon Magnesium chelatase subunit I (CHLI).These results also support that, although potyviruses express a strong silencing suppressor that usually precludes VIGS, mild isolates of this kind of viruses can be used as VIGS vectors.Finally, to facilitate the use of this new tool by cucurbit geneticists, we describe plasmid pGWMV-VIGS that allows easy cloning fragments of the genes of interest in a single Gibson assembly reaction.

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Applications of nanobodies in plant science and biotechnology

Cited by 27 publications

References 99 publications

Phytonanotechnology applications in modern agriculture

Phytonanotechnology applications in modern agriculture

Nanobodies: their potential for applications in biotechnology, diagnosis and antiviral properties in Africa; focus on application in agriculture

Biotechnological Application of Potyvirus-Based Vectors in Cucurbit Plants

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