Applications of MALDI-TOF-MS in structural characterization of synthetic polymers

Sun, Abin; Li, Siting; Kou, Xinhui

doi:10.1039/d2ay01583h

Cited by 4 publications

(1 citation statement)

References 144 publications

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“…However, detergent could damage the performance of chromatographic column and is generally incompatible with electrospray mass spectrometer (ESI-MS) due to signal suppression or enhancement (Furey et al 2013 ; Loo et al 1996 ). Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) has a higher tolerance to detergent as the samples are detected in crystals (Cheon et al 2021 ; Sun et al 2023 ). We can assess the concentration and type of detergents using MALDI-TOF-MS as the mass spectra of samples would display different patterns in mass-to-charge ratios ( m / z ) below 1,000 and/or upper 10,000.…”

Section: Overview Of the Protocolmentioning

confidence: 99%

A hydrogen–deuterium exchange mass spectrometry-based protocol for protein–small molecule interaction analysis

Meng,

Song,

Zhou

et al. 2023

Biophysics Reports

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Protein–small molecule interaction is vital in regulating protein functions and controlling various cellular processes. Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful methodology to study protein–small molecule interactions, however, to accurately probe the conformational dynamics of the protein upon small molecule binding, the HDX-MS experimental conditions should be carefully controlled and optimized. Here, we present the detailed continuous-labeling, bottom-up HDX-MS protocol for studying protein–small molecule interactions. We took a side-by-side HDX kinetics comparison of the Hsp90N protein with or without the treatment of small molecules ( i . e ., Radicicol, Geldanamycin) for displaying conformational changes induced by molecular interactions between Hsp90N and small molecules. Our sensitive and robust experimental protocol can facilitate the novice to quickly carry out the structural characterization of protein–small molecule interactions.

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Section: Overview Of the Protocolmentioning

confidence: 99%

A hydrogen–deuterium exchange mass spectrometry-based protocol for protein–small molecule interaction analysis

Meng,

Song,

Zhou

et al. 2023

Biophysics Reports

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show abstract

A pH-responsive cetuximab-conjugated DMAKO-20 nano-delivery system for overcoming K-ras mutations and drug resistance in colorectal carcinoma

Song,

Li,

Shen

et al. 2024

Acta Biomaterialia

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A Bio-Responsive Cetuximab-Conjugated Dmako-20 Nano-Delivery System for Overcoming The K-Ras Mutations And Drug Resistance In Colorectal Carcinoma

Song,

Li,

Shen

et al. 2023

Preprint

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Applications of MALDI-TOF-MS in structural characterization of synthetic polymers

Abstract: In recent years, matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has been utilized to fast and precisely characterize the detailed molecular structures of synthetic polymers. This review summarizes recent...

Cited by 4 publications

References 144 publications

A hydrogen–deuterium exchange mass spectrometry-based protocol for protein–small molecule interaction analysis

A hydrogen–deuterium exchange mass spectrometry-based protocol for protein–small molecule interaction analysis

A pH-responsive cetuximab-conjugated DMAKO-20 nano-delivery system for overcoming K-ras mutations and drug resistance in colorectal carcinoma

A Bio-Responsive Cetuximab-Conjugated Dmako-20 Nano-Delivery System for Overcoming The K-Ras Mutations And Drug Resistance In Colorectal Carcinoma

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