Applications of infrared spectroscopy in polysaccharide structural analysis: Progress, challenge and perspective

Hong, Tao; Yin, Jingdong; Nie, Shaoping; Xie, Mingyong

doi:10.1016/j.fochx.2021.100168

Cited by 187 publications

(109 citation statements)

References 128 publications

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“…SH groups showed potential activity as reducing agents and could contribute to the biosynthesis and stabilization of the AgNPs [31]. The band at 2842 cm −1 (weak) could be assigned to stretching vibrations of skeletal CH 2 in polysaccharides [32] or fatty acids [33]. Both polysaccharides and fatty acids could play a role in the biosynthesis or stabilization of AgNPs [34][35][36].…”

Section: Effect Of Agnps Biosynthesized By Synechocystis Sp On the Inflammatory Mediatorsmentioning

confidence: 99%

Green Synthesis of Silver Nanoparticles by the Cyanobacteria Synechocystis sp.: Characterization, Antimicrobial and Diabetic Wound-Healing Actions

2022

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Green nanotechnology is now accepted as an environmentally friendly and cost-effective advance with various biomedical applications. The cyanobacterium Synechocystis sp. is a unicellular spherical cyanobacterium with photo- and hetero-trophic capabilities. This study investigates the ability of this cyanobacterial species to produce silver nanoparticles (AgNPs) and the wound-healing properties of the produced nanoparticles in diabetic animals. Methods: UV–visible and FT-IR spectroscopy and and electron microscopy techniques investigated AgNPs’ producibility by Synechocystis sp. when supplemented with silver ion source. The produced AgNPs were evaluated for their antimicrobial, anti-oxidative, anti-inflammatory, and diabetic wound healing along with their angiogenesis potential. Results: The cyanobacterium biosynthesized spherical AgNPs with a diameter range of 10 to 35 nm. The produced AgNPs exhibited wound-healing properties verified with increased contraction percentage, tensile strength and hydroxyproline level in incision diabetic wounded animals. AgNPs treatment decreased epithelialization period, amplified the wound closure percentage, and elevated collagen, hydroxyproline and hexosamine contents, which improved angiogenesis factors’ contents (HIF-1α, TGF-β1 and VEGF) in excision wound models. AgNPs intensified catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities, and glutathione (GSH) and nitric oxide content and reduced malondialdehyde (MDA) level. IL-1β, IL-6, TNF-α, and NF-κB (the inflammatory mediators) were decreased with AgNPs’ topical application. Conclusion: Biosynthesized AgNPs via Synechocystis sp. exhibited antimicrobial, anti-oxidative, anti-inflammatory, and angiogenesis promoting effects in diabetic wounded animals.

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Section: Effect Of Agnps Biosynthesized By Synechocystis Sp On the Inflammatory Mediatorsmentioning

confidence: 99%

Green Synthesis of Silver Nanoparticles by the Cyanobacteria Synechocystis sp.: Characterization, Antimicrobial and Diabetic Wound-Healing Actions

2022

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“…The functional groups present in the EPSwk were investigated with FTIR spectroscopy. The results illustrated in the FTIR spectrum (Figure 2a) confirmed that the analyzed material is a polysaccharide due to the presence of absorption bands in five specific regions: region Ⅰ (4000-2500 cm −1 ), region Ⅱ (1800-1500 cm −1 ), region III (1500-1200 cm −1 ), region Ⅳ (1200-800 cm −1 ), and region Ⅴ (below 800 cm −1 ) [68]. The high-intensity band at 3371 cm −1 corresponds to O─H stretching vibrations [62].…”

Section: Fourier Transform Infrared Spectroscopy (Ftir)mentioning

confidence: 53%

Biopolymer from Water Kefir as a Potential Clean-Label Ingredient for Health Applications: Evaluation of New Properties

et al. 2022

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The present work aimed to characterize the exopolysaccharide obtained from water kefir grains (EPSwk), a symbiotic association of probiotic microorganisms. New findings of the technological, mechanical, and biological properties of the sample were studied. The EPSwk polymer presented an Mw of 6.35 × 105 Da. The biopolymer also showed microcrystalline structure and characteristic thermal stability with maximum thermal degradation at 250 °C. The analysis of the monosaccharides of the EPSwk by gas chromatography demonstrated that the material is composed of glucose units (98 mol%). Additionally, EPSwk exhibited excellent emulsifying properties, film-forming ability, a low photodegradation rate (3.8%), and good mucoadhesive properties (adhesion Fmax of 1.065 N). EPSwk presented cytocompatibility and antibacterial activity against Escherichia coli and Staphylococcus aureus. The results of this study expand the potential application of the exopolysaccharide from water kefir as a potential clean-label raw material for pharmaceutical, biomedical, and cosmetic applications.

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“…Three absorption peaks in the “fingerprint” regions at 1,144, 1,100, and 1,017 cm –1 indicated that sugars in GPS2b existed as pyranose forms. Additionally, absorptions at around 832 and 916 cm –1 indicated that the present form of anomeric carbon was mainly α configuration ( 29 ). FT-IR spectra of other fractions were displayed in Figures 2C,D .…”

Section: Resultsmentioning

confidence: 99%

Structural Characterization of a Low Molecular Weight HG-Type Pectin From Gougunao Green Tea

et al. 2022

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Tea is a popular beverage with a long history of safe and healthy use. Tea polysaccharide is a bioactive component extracted from tea, which has attracted more and more attention in recent decades. In this article, an acidic polysaccharide Gougunao tea polysaccharide (GPS) was isolated from Gougunao green tea by hot water extraction and ethanol precipitation. After purification by a diethylaminoethyl (DEAE) Sepharose Fast Flow column and a Sephacryl S-400 column, several homogalacturonan (HG) and rhamnogalacturonan-I (RG-I) fractions were obtained. Fraction GPS2b with the highest yield was selected for structural characterization by methylation and nuclear magnetic resonance (NMR) analysis. GPS2b was found to be an HG-type pectic polysaccharide (degree of methyl esterification [DE], 51.6%) with low molecular weight (Mw, 36.8 kDa). It was mainly composed of →4)-α-GalpA- (1→ and →4)-α-GalpA-6-OMe-(1→. In addition, a minor highly branched RG-I domain was identified in this fraction. The investigation of structural features of tea polysaccharides can provide insights to understand their structure-bioactivity relationship.

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Applications of infrared spectroscopy in polysaccharide structural analysis: Progress, challenge and perspective

Cited by 187 publications

References 128 publications

Green Synthesis of Silver Nanoparticles by the Cyanobacteria Synechocystis sp.: Characterization, Antimicrobial and Diabetic Wound-Healing Actions

Green Synthesis of Silver Nanoparticles by the Cyanobacteria Synechocystis sp.: Characterization, Antimicrobial and Diabetic Wound-Healing Actions

Biopolymer from Water Kefir as a Potential Clean-Label Ingredient for Health Applications: Evaluation of New Properties

Structural Characterization of a Low Molecular Weight HG-Type Pectin From Gougunao Green Tea

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