Applications of gold nanoparticles in ELISA, PCR, and immuno-PCR assays: A review

Tabatabaei, Mahdis Sadat; Islam, Rafiq; Ahmed, Marya

doi:10.1016/j.aca.2020.08.030

Cited by 95 publications

(53 citation statements)

References 108 publications

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“…9 The uorescence intensity is decreased with the target protein concentration, which causes ELISA is not able to discover the abnormal expression of proteins at the early stage of diseases. 10 To amplify the uorescence at low concentration, various uorescence-signal enhancing approaches are developed, including immuno-PCR, 11,12 quantum dot (QD) labeling, 13,14 plasmon or dielectric resonance enhancing, 15,16 and ultra-small volume conning detection. 17,18 A portion of techniques increase the sensitivity 100-1000 folds and a few LODs reach femtomolar level.…”

Section: Introductionmentioning

confidence: 99%

Ball-lens assisted sensitivity improvement of fluorescence immunoassay in microchannels

Zhang

et al. 2021

RSC Adv.

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Section: Introductionmentioning

confidence: 99%

Ball-lens assisted sensitivity improvement of fluorescence immunoassay in microchannels

Zhang

et al. 2021

RSC Adv.

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“…CANi was designed according to conventional sandwich immunoassay ( Figure 1A ), which measured the antigen between two layers of antibodies (capture and detection antibody) ( Itoh et al, 2002 ; Tabatabaei et al, 2020 ). The capture antibody was immobilized on a 96-well polystyrene surface to capture the analytes of interest.…”

Section: Resultsmentioning

confidence: 99%

“…Various state-of-the-art technologies were combined with the traditional immunoassay and applied in clinical diagnostics, such as a single-molecule array (Simoa) ( Mathian et al, 2019 ; Cohen et al, 2020 ) and rolling circle amplification (RCA) ( Bhat and Rao, 2020 ; Hadi et al, 2020 ). Among them, enzyme-linked immunosorbent assay (ELISA) is the gold standard of in vitro diagnostics to analyze biomarkers and important analytes in healthcare and diversified analytical settings ( Tabatabaei et al, 2020 ). Compared to other immunoassay methods, ELISA has many advantages, such as being sensitive, specific, and high throughput ( Tighe et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Study on Factors Affecting the Performance of a CRISPR/Cas-Assisted New Immunoassay: Detection of Salivary Insulin as an Example

Lin

Wang

et al. 2021

Front. Bioeng. Biotechnol.

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The clustered regularly interspaced short palindromic repeat (CRISPR)/Cas is now playing a significant role in biosensing applications, especially when the trans-cleavage activity of several Cas effectors is discovered. Taking advantages of both CRISPR/Cas and the enzyme-linked immunosorbent assay (ELISA) in analytical and clinical investigations, CRISPR/Cas-powered ELISA has been successfully designed to detect a spectrum of analytes beyond nucleic acid. Herein, we developed a CRISPR/Cas12a-assisted new immunoassay (CANi) for detection of salivary insulin as an example. Specifically, factors (antibody selection, temperature, and assay time) affecting the CRISPR/Cas-based ELISA system’s performance were investigated. It was observed that the concentration of blocking solution, selection of the capture antibody pairs, and the sequences of triggering ssDNA and guiding RNA affected this immunoassay sensitivity. In contrast, the preincubation of CRISPR/Cas12a working solution and pre-mixture of detection antibody with anti-IgG–ssDNA did not show influence on the performance of CANi for the detection of insulin. Under optimized conditions, the sensitivity for detection of salivary insulin was 10 fg/ml with a linear range from 10 fg/ml to 1 ng/ml.

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“…An improvement of up to five orders of magnitude, detecting as less as 10 −21 moles (zepto moles; closing into the number of Avogadro! ), is obtained by replacing the (enzyme-)label-conjugate by an oligonucleotide that can be amplified exponentially by a usual PCR reaction [ 135 , 136 ]. This technique called immuno-PCR (iPCR) exploits the flexibility and versatility of an ELISA and the signal amplification ability of a PCR reaction.…”

Section: Analytical Microbiologymentioning

confidence: 99%

Modernization of Control of Pathogenic Micro-Organisms in the Food-Chain Requires a Durable Role for Immunoaffinity-Based Detection Methodology—A Review

Bergwerff

Debast

2021

Foods

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Food microbiology is deluged by a vastly growing plethora of analytical methods. This review endeavors to color the context into which methodology has to fit and underlines the importance of sampling and sample treatment. The context is that the highest risk of food contamination is through the animal and human fecal route with a majority of foodborne infections originating from sources in mass and domestic kitchens at the end of the food-chain. Containment requires easy-to-use, failsafe, single-use tests giving an overall risk score in situ. Conversely, progressive food-safety systems are relying increasingly on early assessment of batches and groups involving risk-based sampling, monitoring environment and herd/flock health status, and (historic) food-chain information. Accordingly, responsible field laboratories prefer specificity, multi-analyte, and high-throughput procedures. Under certain etiological and epidemiological circumstances, indirect antigen immunoaffinity assays outperform the diagnostic sensitivity and diagnostic specificity of e.g., nucleic acid sequence-based assays. The current bulk of testing involves therefore ante- and post-mortem probing of humoral response to several pathogens. In this review, the inclusion of immunoglobulins against additional invasive micro-organisms indicating the level of hygiene and ergo public health risks in tests is advocated. Immunomagnetic separation, immunochromatography, immunosensor, microsphere array, lab-on-a-chip/disc platforms increasingly in combination with nanotechnologies, are discussed. The heuristic development of portable and ambulant microfluidic devices is intriguing and promising. Tant pis, many new platforms seem unattainable as the industry standard. Comparability of results with those of reference methods hinders the implementation of new technologies. Whatever the scientific and technological excellence and incentives, the decision-maker determines this implementation after weighing mainly costs and business risks.

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Applications of gold nanoparticles in ELISA, PCR, and immuno-PCR assays: A review

Cited by 95 publications

References 108 publications

Ball-lens assisted sensitivity improvement of fluorescence immunoassay in microchannels

Ball-lens assisted sensitivity improvement of fluorescence immunoassay in microchannels

Study on Factors Affecting the Performance of a CRISPR/Cas-Assisted New Immunoassay: Detection of Salivary Insulin as an Example

Modernization of Control of Pathogenic Micro-Organisms in the Food-Chain Requires a Durable Role for Immunoaffinity-Based Detection Methodology—A Review

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