Applications of Fluorescent Brighteners in Biological Techniques

Darken, Marjorie A.

doi:10.1126/science.133.3465.1704

Cited by 77 publications

(26 citation statements)

References 6 publications

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“…19,[51][52][53] The fluorescence of chitin-binding dye is generated when it is intercalated between sugar-ring structures, particularly chitin. As shown in Fig.…”

Section: )mentioning

confidence: 99%

Effect ofBombyx moriChitinase against Japanese Pine Sawyer (Monochamus alternatus) Adults as a Biopesticide

Kabir¹,

Sugimoto²,

Tado³

et al. 2006

Bioscience, Biotechnology, and Biochemistry

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“…19,[51][52][53] The fluorescence of chitin-binding dye is generated when it is intercalated between sugar-ring structures, particularly chitin. As shown in Fig.…”

Section: )mentioning

confidence: 99%

Effect ofBombyx moriChitinase against Japanese Pine Sawyer (Monochamus alternatus) Adults as a Biopesticide

Kabir¹,

Sugimoto²,

Tado³

et al. 2006

Bioscience, Biotechnology, and Biochemistry

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“…The stains used are Calcofluor, which stains chitin and other f~-linked polysaccharides (Darken 1961;Maeda and Ishida 1967), and the DNA stain Hoechst 33258. Chitin is a major …”

mentioning

confidence: 99%

A morphological and genetic analysis of conidiophore development in Neurospora crassa.

Springer¹,

Yanofsky²

1989

Genes Dev.

146

156

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The filamentous fungus Nearospora crassa responds to nutrient deprivation and dessication by producing asexual spores, or conidia. These conidia are derived from differentiated aerial structures called conidiophores. The process of conidiation was analyzed in wild-type and morphological mutants using scanning electron microscopy (SEM) and specific fluorescent probes. The first discernible morphological step of conidiation is the transition from growth by hyphal tip elongation to growth by repeated apical budding, resulting in the formation of chains of proconidia that resemble beads on a string. The initial proconidial chains are morphologically distinct from those that form later and are capable of reverting to hyphal growth, whereas the later chains are committed to conidiation. As the proconidial chains are formed, nuclei migrate into the conidiophore, and cross-walls arise between adjoining proconidia in a series of steps that have been defined by staining with Calcofluor, a fluorescent chitin-binding probe. The chains ultimately disarticulate in several discrete stages into free, morphologically mature conidia. Different conidiation-defective mutants were shown to be blocked at distinct stages in conidiation. Our observations permit us to derive a developmental timeline of conidiation relating the occurrence of morphological changes and the stage blocked in specific mutants.

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“…Numerous adjunct uses have been described for these compounds including fluorochromes for micro-organisms (Darken 1961;Dougherty et al 1996). All the fluorescent brighteners used in this study enhance viral activity and larval mortality almost 400-fold compared a PbGV applied at 2.0 £ 10 5 OBs ml 21 .…”

Section: Discussionmentioning

confidence: 95%

Effect of UV protectants on the efficacy ofPieris brassicaegranulovirus

Sood

Mehta

Prabhakar

2013

Biological Agriculture & Horticulture

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Fluorescent brighteners increase insect viral activity by providing protection against UV inactivation. The studies were conducted to evaluate the effect of some UV protectants on the efficacy of a localized potential Pieris brassicae granulosis virus (PbGV) isolate for its effective field usage. Laboratory studies determined the effect of UV protectants on median inactivation time of virus and inhibition of virus activity of the test isolate. UV protectants, robin blue, congo red, and tinopal were effective in minimizing the detrimental effect of UV radiations on PbGV in suspension. Promising combinations were evaluated under field conditions at two locations in India, Palampur (sub-tropical) and Sangla (temperate), on three cole crops (cabbage, cauliflower, and broccoli). Congo red followed by robin blue and tinopal was found to be the most promising candidate for enhancing the PbGV efficacy by protection from UV radiation.

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Applications of Fluorescent Brighteners in Biological Techniques

Abstract: The visual labeling of various microorganisms has been accomplished with specific fluorescent ultraviolet-absorbing compounds that not only have been bound by the cell but have been transferred by it to subsequent growth. The possibility of application to genetic studies is suggested.

Cited by 77 publications

References 6 publications

Effect ofBombyx moriChitinase against Japanese Pine Sawyer (Monochamus alternatus) Adults as a Biopesticide

Effect ofBombyx moriChitinase against Japanese Pine Sawyer (Monochamus alternatus) Adults as a Biopesticide

A morphological and genetic analysis of conidiophore development in Neurospora crassa.

Effect of UV protectants on the efficacy ofPieris brassicaegranulovirus

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