Applications of Digital Holography in Biomedical Microscopy

Kim, Myung-K.

doi:10.3807/josk.2010.14.2.077

Cited by 51 publications

(19 citation statements)

References 90 publications

(98 reference statements)

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“…Recently, Min et al has proposed a numerical correction method to rejuvenate the degraded OCT images. 41 The method uses the phase-shifting digital holographic technique 42 based on the Fresnel-Kirchhoff diffraction theory, which numerically relocates the defocused sample at the virtual focal plane. Ideally, a fully focused OCT image can be constructed regardless of the degree of optical distortion along the depth of the sample.…”

Section: Conclusion and Discussionmentioning

confidence: 99%

Qualitative investigation of fresh human scalp hair with full-field optical coherence tomography

Choi

Pi²,

Min

et al. 2012

J. Biomed. Opt.

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Abstract. We have investigated depth-resolved cellular structures of unmodified fresh human scalp hairs with ultrahigh-resolution full-field optical coherence tomography (FF-OCT). The Linnik-type white light interference microscope has been home-implemented to observe the micro-internal layers of human hairs in their natural environment. In hair shafts, FF-OCT has qualitatively revealed the cellular hair compartments of cuticle and cortex layers involved in keratin filaments and melanin granules. No significant difference between black and white hair shafts was observed except for absence of only the melanin granules in the white hair, reflecting that the density of the melanin granules directly affects the hair color. Anatomical description of plucked hair bulbs was also obtained with the FF-OCT in three-dimensions. We expect this approach will be useful for evaluating cellular alteration of natural hairs on cosmetic assessment or diagnosis of hair diseases.

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Section: Conclusion and Discussionmentioning

confidence: 99%

Qualitative investigation of fresh human scalp hair with full-field optical coherence tomography

Choi

Pi²,

Min

et al. 2012

J. Biomed. Opt.

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“…It is a quantitative phase microscopy technique capable of being configured for reflection or transmission microscopy, and it has been used for a variety of applications [1], including static and dynamic surface metrology [2][3][4][5][6][7], particle tracking [8,9], tracking and monitoring of live biological cells [3,[10][11][12], and monitoring surface dissolution [13,14] or growth [15] kinetics. DHM and related quantitative phase imaging technologies have been extensively developed for the study of biological specimens [16][17][18]. The DHM operates by separating and recombining object and reference beams at a single wavelength to generate the interferometric data as a hologram, which is then numerically reconstructed to yield amplitude and phase information [19][20][21], examples of which are shown in The primary advantage of DHM is that full-field two-dimensional (2D) holograms are collected at tens of frames per second and can be collected in situ.…”

Section: Introductionmentioning

confidence: 99%

Phase Uncertainty in Digital Holographic Microscopy Measurements in the Presence of Solution Flow Conditions

Brand¹

2017

J. RES. NATL. INST. STAN.

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Digital holographic microscopy (DHM) is a surface topography measurement technique with reported sub-nanometer vertical resolution. Although it has been made commercially available recently, few studies have evaluated the uncertainty or noise in the phase measurement by the DHM. As current research is using the DHM to monitor surface topography changes of dissolving materials under flowing water conditions, it is necessary to evaluate the effect of water and flow rate on the uncertainty in the measurement. Uncertainty in this study was concerned with the temporal standard deviation per pixel of the reconstructed phase. Considering the effects of solution flow rate, magnification, objective lens type (air or immersion), and experimental configuration, measurements under static conditions in air and in water with an immersion lens yielded the smallest amount of uncertainty (mean of ≤ 0.5 nm up to 40× magnification). Increasing the water flow rate resulted in an increase in mean uncertainty to ≤ 0.6 nm up to 40× with an immersion lens. Observations of a sample through a glass window at 20× magnification in flowing water also yielded increasing uncertainty, with mean values of ≤ 0.5 nm, ≤ 0.8 nm, and ≤ 1.1 nm for flow rates of 0 mL min ) did not significantly impact the uncertainty in the phase. Collecting holograms in single-wavelength versus dual-wavelength modes did impact the uncertainty, with the mean uncertainty at 10× magnification for the same wavelength being ≤ 0.5 nm from the single-wavelength mode compared to ≤ 1.5 nm from the dualwavelength mode. When the quantified uncertainty was applied to simulated dissolution data, lower limits of measured dissolution rates were found below which the measured data may not be distinguishable from the uncertainty in the measurement. The limiting surface-normal dissolution velocity is −10 −11.7 m s ) flowing water conditions in experiments with a glass window, respectively. The data presented by this study will allow for better experimental design and methodology for future dissolution or precipitation studies using DHM and will provide confidence in the data produced in postprocessing.

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“…The process of producing a hologram that is digitally recorded and numerically reconstructed, known as Digital holography (DH) [1], has become an opto-digital technique with various applications among which are mechanics, biomedicine, microscopy or spectroscopy [2][3][4][5][6][7][8][9][10][11]. This increased use comes from the fact that DH offers the possibility of accessing phase and amplitude of the optical field in a quantitative manner, fact that is a differential feature when compared with other imaging techniques.…”

Section: Introductionmentioning

confidence: 99%

Assessing digital micromirror devices for speckle noise control in digital holography

Gaviria-Mesa¹,

Hincapie²,

Garcı́a-Sucerquia³

et al. 2017

Opt. Pura Apl.

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ABSTRACT:Speckle noise is a common issue in coherent imaging techniques. As one of these techniques, digital holography (DH) is affected by this problem. Although several attempts have been made to overcome speckle noise in the numerical reconstruction of digitally recorded holograms, there still exists the need for fast and effective methods to get rid of speckle and improve image quality. We assess the suitability of a digital micromirror device (DMD) as modulator of the object illumination in a digital holographic setup, where the DMD acts generating speckle-like patterns that are projected onto the object. These patterns change the object beam in a random way. Several holograms, each with a different pattern in the object illumination, are recorded and numerically reconstructed. The image with reduced speckle noise is the result of a superposition in an intensity basis of various such reconstructions. The feasibility of the method is validated by experimental results.Key words: Digital holography; Speckle; Digital Micromirror Device RESUMEN:El ruido speckle es un problema inherente a las técnicas de formación de imágenes con luz coherente. Ya que la holografía digital es una de estas técnicas, también se ve afectada por este problema. Aunque se han hecho varios intentos por eliminar el ruido speckle en la reconstrucción numérica de hologramas registrados digitalmente, todavía existe la necesidad de métodos rápidos y efectivos para eliminar el speckle y mejorar la calidad de las imágenes reconstruidas. Nosotros evaluamos la conveniencia del uso de un dispositivo digital de microespejos (DMD) como modulador de la iluminación del objeto en un montaje holográfico digital. El DMD actúa generando patrones aleatorios tipo speckle que son proyectados sobre el objeto. Estos patrones cambian el haz objeto de una manera aleatoria y después interfiere formando el holograma. Se registran varios hologramas, cada uno con un patrón diferente en la iluminación del objeto, y se reconstruyen numéricamente. La imagen final con el ruido speckle reducido es el resultado de la superposición en intensidades de varias de estas reconstrucciones. La viabilidad del método es validada mediante resultados experimentales. [28] X. Cai and H. Wang, "The influence of hologram aperture on speckle noise in the reconstructed image of digital holography and its reduction", Opt. Commun. 281, 232-237 (2008).

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Applications of Digital Holography in Biomedical Microscopy

Cited by 51 publications

References 90 publications

Qualitative investigation of fresh human scalp hair with full-field optical coherence tomography

Qualitative investigation of fresh human scalp hair with full-field optical coherence tomography

Phase Uncertainty in Digital Holographic Microscopy Measurements in the Presence of Solution Flow Conditions

Assessing digital micromirror devices for speckle noise control in digital holography

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