Applications of Dielectrophoresis in the Field of Medical Sciences

Arya, Riteshkumar; Komal, Hirani; Sankaranarayanan, A.; Krishnamurthy, R.

doi:10.32628/ijsrst18401161

Cited by 7 publications

(8 citation statements)

References 47 publications

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“…There is a need for new technologies that allow for faster microbial detection and assessment of microbial contamination.Dielectrophoresis (DEP) has shown great promise for particle separation for decades (see excellent reviews [3][4][5] ). Several DEP systems show promising potential applications in medical sciences, including drug delivery or cancer diagnostics [6][7][8][9][10][11] . However, DEP has not yet been widely applied in clinical settings.…”

mentioning

confidence: 99%

Fluid-Screen as a real time dielectrophoretic method for universal microbial capture

Weber

Petkowski

Michaels

et al. 2021

Sci Rep

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Bacterial culture methods, e.g. Plate Counting Method (PCM), are a gold standard in the assessment of microbial contamination in multitude of human industries. They are however slow, labor intensive, and prone to manual errors. Dielectrophoresis (DEP) has shown great promise for particle separation for decades; however, it has not yet been widely applied in routine laboratory setting. This paper provides an overview of a new DEP microbial capture and separation method called Fluid-Screen (FS), that achieves very fast, efficient, reliable and repeatable capture and separation of microbial cells. Method verification experiments demonstrated that the FS system captured 100% of bacteria in test samples, a capture efficiency much higher than previously reported for similar technology. Data generated supports the superiority of the FS method as compared to the established Plate Counting Method (PCM), that is routinely used to detect bacterial contamination in healthcare, pharmacological and food industries. We demonstrate that the FS method is universal and can capture and separate different species of bacteria and fungi to viruses, from various sample matrices (i.e. human red blood cells, mammalian cells).

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mentioning

confidence: 99%

Fluid-Screen as a real time dielectrophoretic method for universal microbial capture

Weber

Petkowski

Michaels

et al. 2021

Sci Rep

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“…In conclusion, the suspending medium conductivity significantly impacts the discrimination of DEP cells despite the DEP f XO [6]. DEP discriminates against different cells based on surface morphology (surface area, morphology, and size).…”

Section: Conductive Medium Impact On Depmentioning

confidence: 99%

“…It provides a wide range of media conductivities and voltages that can be applied to enrich targeted particles by DEP rapidly [114]. It is worth mentioning that using 3D electrode structures, ion‐exchange membranes, or covering the electrodes with a thin layer of insulating material is critical in reducing the side effects of high‐conductive media in enrichment systems [6].…”

Section: Conductive Medium Impact On Depmentioning

confidence: 99%

A correlation of conductivity medium and bioparticle viability on dielectrophoresis‐based biomedical applications

et al. 2023

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Dielectrophoresis (DEP) bioparticle research has progressed from micro to nano levels. It has proven to be a promising and powerful cell manipulation method with an accurate, quick, inexpensive, and label-free technique for therapeutic purposes. DEP, an electrokinetic phenomenon, induces particle movement as a result of polarization effects in a nonuniform electrical field. This review focuses on current research in the biomedical field that demonstrates a practical approach to DEP in terms of cell separation, trapping, discrimination, and enrichment under the influence of the conductive medium in correlation with bioparticle viability. The current review aims to provide readers with an in-depth knowledge of the fundamental theory and principles of the DEP technique, which is influenced by conductive medium and to identify and demonstrate the biomedical application areas. The high conductivity of physiological fluids presents obstacles and opportunities, followed by bioparticle viability in an electric field elaborated in detail. Finally, the drawbacks of DEP-based systems

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“…Dielectrophoresis (DEP) has shown great promise for particle separation for decades (see excellent reviews [3][4][5] ). Several DEP systems show promising potential applications in medical sciences, including drug delivery or cancer diagnostics [6][7][8][9][10][11] . However, DEP has not yet been widely applied in clinical settings.…”

Section: Introductionmentioning

confidence: 99%

Fluid-Screen as a Real Time Dielectrophoretic Method for Universal Microbial Capture

Weber

Petkowski

et al. 2021

Preprint

View full text Add to dashboard Cite

Dielectrophoresis (DEP) has shown great promise for particle separation for decades; however, it has not yet been widely applied in routine laboratory setting. This paper provides an overview of a new DEP microbial capture and separation method called Fluid-Screen (FS), that achieves an efficient, reliable and repeatable capture and separation of microbial cells. Method verification experiments demonstrated that the FS system captured 100% of bacteria in test samples, a capture efficiency much higher than previously reported for similar technology. Data generated supports the superiority of the FS method as compared to the established Plate Counting Method (PCM), that is routinely used to detect bacterial contamination in healthcare, pharmacological and food industries. We have established that the FS method is universal and can capture and separate different species of bacteria and fungi to viruses, from various sample matrices (i.e. human red blood cells, mammalian cells).

show abstract

Applications of Dielectrophoresis in the Field of Medical Sciences

Cited by 7 publications

References 47 publications

Fluid-Screen as a real time dielectrophoretic method for universal microbial capture

Fluid-Screen as a real time dielectrophoretic method for universal microbial capture

A correlation of conductivity medium and bioparticle viability on dielectrophoresis‐based biomedical applications

Fluid-Screen as a Real Time Dielectrophoretic Method for Universal Microbial Capture

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