Applications of dielectrophoresis in biotechnology

Pethig, Ronald; Markx, Gerard H.

doi:10.1016/s0167-7799(97)01096-2

Cited by 322 publications

(249 citation statements)

References 31 publications

Supporting

Mentioning

245

Contrasting

Order By: Relevance

“…To achieve these, various techniques have been developed to be used in microsystems such as optical tweezers [1], magnetophoresis [2], acoustic means [3,4,5] and dielectrophoresis (DEP). Among these, DEP is one of the most popular methods for particle manipulation in microsystems due to (i) its favorable scaling effects [6], (ii) the simplicity of the instrumentation and (iii) its ability to induce both negative and positive forces [7]. DEP is the movement of particles in non-uniform electrical fields due to the interaction of the particle's dipole and spatial gradient of the electrical field.…”

Section: Introductionmentioning

confidence: 99%

Continuous particle separation based on electrical properties using alternating current dielectrophoresis

Çetin

2009

Electrophoresis

View full text Add to dashboard Cite

A design of a microchannel for continuous separation of particles based on alternating current dielectrophoretic is studied. The geometric design parameters are determined by analyzing the dielectrophoresis force field inside the microchannel corresponding to the most efficient separation. The trajectories of 5 and 10 mm spherical particles are derived analytically using Lagrangian tracking method to examine the feasibility and the effectiveness of the design. The effects of the mean flow velocity inside the channel and the applied voltage across the channel on the performance of the separation are also discussed.

show abstract

Section: Introductionmentioning

confidence: 99%

Continuous particle separation based on electrical properties using alternating current dielectrophoresis

Çetin

2009

Electrophoresis

View full text Add to dashboard Cite

show abstract

“…As prokaryotes, bacteria do not have membrane-bound organelles in their cytoplasm and thus contain few large intracellular structures. Accordingly, they lack a nucleus, mitochondria, chloroplasts and other organelles which are otherwise present in eukaryotic cells [12]. While the cell membrane is highly insulated, the interior of the cell is highly conductive [5].…”

mentioning

confidence: 99%

“…While the cell membrane is highly insulated, the interior of the cell is highly conductive [5]. A study has shown that the conductivity of the cell membrane is around 10 −7 S/m, whereas that of the interior of a cell can be as high as 1 S/m [12]. The bacterial impedance based technology was shown to have detection limits of 10 2 to 10 6 cfu/mL for various bacterial cells with a detection time from 30 min to several hours [5].…”

mentioning

confidence: 99%

Development of a novel conductance-based technology for environmental bacterial sensing

Shen

Tan

et al. 2013

Chin. Sci. Bull.

View full text Add to dashboard Cite

In this study, a simple impedance based technology for measuring bacterial concentrations was developed. The measurement system includes the signal amplification, copper probes and a sample loader. During the experiments, the conductance of Bacillus subtilis var niger, Pseudomonas fluorescens, and Escherichia coli were measured using the combination of a pre-amplifier and a lock-in amplifier. The conductance data were modeled verses the bacterial concentrations. Results indicated that the relationship between the conductance of bacterial suspensions and their concentrations follows a generic model: Y=C 1 + C 2 × e (−X/C 3 ) , where Y is the conductance (S), X is the bacterial concentration (Number/mL: abbreviated to N/mL) for all species tested, and C 1−3 are constants. Gram negative P. fluorescens and E. coli assumed similar conductance curves, which were flatter than that of gram positive B. subtilis var niger. For P. fluorescens and E. coli the culturing technique resulted in higher concentration levels (statistically significant) from 2 to 4 times that measured by the impedance based technology. For B. subtilis var niger, both methods resulted in similar concentration levels. These differences might be due to membrane types, initial culturability and the obtained conductance curves. The impedance based technology here was shown to obtain the bacterial concentration instantly, holding broad promise in realtime monitoring biological agents.

show abstract

“…Dielectrophoretic (DEP) microfilters are based on the movement of polarisable particles in non-uniform fields and have applications as trapping and separation of cells, viruses or macromolecules [1][2][3][4][5][6]. The advanced microfabrication techniques have led to rapid development of various types of microelectrodes that can manipulate micro and nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Design and Coupled Electro-Fluidic Simulation of a Novel Dielectrophoretic Microfilter

Nedelcu

2011

Acta Phys. Pol. A

View full text Add to dashboard Cite

In this paper a design of a new dielectrophoretic microfilter is proposed, based on electrodes configuration having stairs geometry that multiply the opposite points of electrodes where minimal and maximal values of electric field are obtained. The results of electrostatic simulation show very well defined regions of extremes values where particles can be separated. A model for particle-fluid interaction is used in momentum equation as reaction body force depending on electric field and particle concentration. The fluid flow and particles separation were simulated for positive dielectrophoresis, based on electrostatic results by means of dielectrophoretic velocity and reaction force acting on suspending media. The distribution of particle concentration depends on dielectrophoretic velocity and flow velocity, but also it influences the flow velocity, hence these fields were solved in coupled approach. The resulting flow profile, streamlines and concentration distribution demonstrate that particle migration influences the surrounding fluid and leads to modified velocity distribution by comparison to classical fluid flow.

show abstract

Applications of dielectrophoresis in biotechnology

Cited by 322 publications

References 31 publications

Continuous particle separation based on electrical properties using alternating current dielectrophoresis

Continuous particle separation based on electrical properties using alternating current dielectrophoresis

Development of a novel conductance-based technology for environmental bacterial sensing

Design and Coupled Electro-Fluidic Simulation of a Novel Dielectrophoretic Microfilter

Contact Info

Product

Resources

About