Applications of CRISPR/Cas9 Technology in the Treatment of Lung Cancer

Jiang, Chunyang; Lin, Xiaohui; Zhao, Zhigang

doi:10.1016/j.molmed.2019.07.007

Cited by 43 publications

(26 citation statements)

References 71 publications

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“…In the past few years, due to the specificity, efficiency, and accuracy of CRISPR/Cas9, this genome editing tool is excessively used in the research laboratories, which helped the researchers to detect the role of different oncogenes in the cancer cells [ 33 ]. The tumor-suppressor genes ( ETS1 , CPEB2 , BRCA1 , PGC1a , TP53 , MiR-1205 , and SOX15 ) comprise their significant role in tumorigenesis, which can suppress cell mitigation, cell proliferation, enhance cell differentiation, and downregulate the cancer progression [ 34 , 35 ]. The CRISPR/Cas9 technology targeted these tumor-suppressor genes to inhibit or reduce the tumorigenesis by restoring the activities of tumor-suppressor genes [ 36 ].…”

Section: The Potential Therapeutic Target Effects Of Crispr/cas9 In Smentioning

confidence: 99%

“…The brown box represents the activation of different tumor suppressor genes. The orange box characterizes the inhibition/suppression of drug-resistance genes by CRISPR/Cas9 in solid tumors [ 31 , 35 , 45 , 70 , 74 , [115] , [116] , [117] , [118] , [119] , [120] , [121] , [122] , [123] , [124] , [125] , [126] , [127] , [128] ]. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)…”

Section: The Potential Therapeutic Target Effects Of Crispr/cas9 In Smentioning

confidence: 99%

“…A recent study reported that both oncogene overexpression and gene mutations are involved in lung tumor growth. However, the utilization of recently developed gene-editing technology (CRISPR/Cas9) could significantly remove lung cancer by targeting the tumor-suppressive genes, genes associated with therapies by inhibitors, oncogenes, and genes associated with chemotherapy drug resistance [ 35 , 56 ].…”

Section: The Potential Therapeutic Target Effects Of Crispr/cas9 In Smentioning

confidence: 99%

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CRISPR/Cas9: A powerful genome editing technique for the treatment of cancer cells with present challenges and future directions

et al. 2020

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Cancer is one of the most leading causes of death and a major public health problem, universally. According to accumulated data, every year, approximately 8.5 million people died because of the lethality of cancer. Recently, a new RNA domain-containing endonuclease-based genome engineering technology, namely the clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein-9 (Cas9) have been proved as a powerful technique in the treatment of cancer due to its multifunctional properties including high specificity, accuracy, time reducing and cost-effective strategies with minimum off-target effects. The present review investigates the overview of recent studies on the newly developed genome-editing strategy, CRISPR/Cas9, as an excellent pre-clinical therapeutic option in the reduction and identification of new tumor target genes in the solid tumors. Based on accumulated data, we revealed that CRISPR/Cas9 significantly inhibited the robust tumor cell growth (breast, lung, liver, colorectal, and prostate) by targeting the oncogenes, tumor-suppressive genes, genes associated to therapies by inhibitors, and genes associated to chemotherapies drug resistance, and suggested that CRISPR/Cas9 could be a potential therapeutic target in inhibiting the tumor cell growth by suppressing the cell-proliferation, metastasis, invasion and inducing the apoptosis during the treatment of malignancies in the near future. The present review also discussed the current challenges, and barriers and proposed future recommendations for a better understanding.

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Section: The Potential Therapeutic Target Effects Of Crispr/cas9 In Smentioning

confidence: 99%

Section: The Potential Therapeutic Target Effects Of Crispr/cas9 In Smentioning

confidence: 99%

Section: The Potential Therapeutic Target Effects Of Crispr/cas9 In Smentioning

confidence: 99%

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CRISPR/Cas9: A powerful genome editing technique for the treatment of cancer cells with present challenges and future directions

et al. 2020

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“…This enzyme cleaves the DNA at the point of interest and allows its sequence to be modified, deleted, or replaced. Consequently, the CRISPR/Cas9 system can be ubiquitously applied to knock out oncogenes and study tumor-suppressor genes and resistance-related genes [ 151 ].…”

Section: Emerging Treatmentsmentioning

confidence: 99%

Nanomedicine in Non-Small Cell Lung Cancer: From Conventional Treatments to Immunotherapy

García-Fernández

Fornaguera

Borrós

2020

Cancers

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Non-small cell lung cancer (NSCLC) remains the most common cause of cancer-related mortality. The heterogeneous nature of this disease hinders its diagnosis and treatment, requiring continuous advances in research aiming to understand its intricate nature. Consequently, the retrospective analysis of conventional therapies has allowed the introduction of novel tools provided by nanotechnology, leading to considerable improvements in clinical outcomes. Furthermore, the development of novel immunotherapies based on the recently understood interaction of the immune system with the tumor highlights the real possibility of definitively treating NSCLC from its early stages. Novel engineering approaches in nanomedicine will enable to overcome the intrinsic limits of conventional and emerging therapies regarding off-site cytotoxicity, specificity, resistance mechanisms, and administration issues. The convergence point of these therapies with nanotechnology lays the foundation for achieving currently unmet needs.

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“…It was used to knock out the beta‐globin gene, the pathogenic gene of β‐hemoglobinopathy, and it also employed rAAV6, as a donor template, to effectively correct the disease‐causing mutation of SCD through homologous recombination . In addition, The CRISPR‐Cas9 gene editing technology has been widely used treatment‐related researches and in the establishment of disease model of many cancers, including pancreatic, lung, gastric, colon, kidney, liver, head and neck, and skin cancers …”

Section: Introductionmentioning

confidence: 99%

Applications of CRISPR‐Cas9 in gynecological cancer research

et al. 2020

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Gynecological cancers pose a significant threat to women's health worldwide, with cervical cancer, ovarian cancer, and endometrial cancer having high incidences.Current gynecological cancer treatment methods mainly include surgery, chemotherapy, radiotherapy, and chemoradiotherapy. The CRISPR-Cas9 gene editing technology as a new therapeutic method has shown tremendous effect in the treatment of other cancers, promoting research on its potential therapeutic effect in gynecological cancer. In this article, we reviewed the current research status of CRISPR-Cas9 technology in gynecological cancer, focusing on the importance of studying the mechanism of CRISPR-Cas9 in gynecological cancer treatment, thereby laying a foundation for further research on its clinical application. K E Y W O R D S cancer treatment, CRISPR-Cas9, gene editing, gynecological cancers 1 | INTRODUCTIONThe CRISPR sequence, a cluster of regularly spaced short palindromic repeats, is a special ordered repeating sequence found in the genome of Escherichia coli. The CRISPR-Cas9 system constitutes the acquired immune system of E. coli. 1 In 2012, scientists discovered that the CRISPR-Cas9 system can produce mature CRISPR RNA (crRNA) and trans-activating crRNA (tracrRNA), which form a composite structure and have a directional guidance function to guide Cas9 to specific DNA sequences, resulting in DNA double-strand breaks. 2 By 2013, scientists had successfully edited the genome of eukaryotic cells using the CRISPR-Cas9 system. [3][4][5] Since then, the CRISPR-Cas9 gene editing technology has dramatically impacted the field of life sciences and has become one of the most significant research areas at present. The working principle of the CRISPR-Cas9 system is to artificially design two kinds of RNA: crRNA and tracrRNA, and then transform them into a single guide RNA (sgRNA), thereby guiding Cas9 to cut DNA in a targeted manner, following which cells are able to delete the target DNA through homologous and non-homologous DNA damage repair mechanisms. 6 Compared with traditional gene editing tools, such as ZFN and TALEN, the CRISPR-Cas9 technology has the advantages of simple design, ability to target any DNA sequence in the cell and to target multiple targets simultaneously, higher cutting efficiency, and lower cost. 7 At present, the CRISPR-Cas9 gene editing technology has contributed to great advancements in the clinical treatment of diseases. Major breakthroughs have been achieved in treating hereditary diseases using this technology. [8][9][10] It was used to knock out the beta-globin gene, the pathogenic gene of β-hemoglobinopathy, and it also employed rAAV6, as a donor template, to effectively correct the disease-causing mutation of SCD through homologous recombination. 10 In addition, The CRISPR-Cas9 gene editing technology has been widely used treatment-related researches and in the establishment of disease model of many cancers, including pancreatic, lung, gastric, colon, kidney, liver, head and neck, and skin cancers. [11][12][13][14][15][16][...

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Applications of CRISPR/Cas9 Technology in the Treatment of Lung Cancer

Cited by 43 publications

References 71 publications

CRISPR/Cas9: A powerful genome editing technique for the treatment of cancer cells with present challenges and future directions

CRISPR/Cas9: A powerful genome editing technique for the treatment of cancer cells with present challenges and future directions

Nanomedicine in Non-Small Cell Lung Cancer: From Conventional Treatments to Immunotherapy

Applications of CRISPR‐Cas9 in gynecological cancer research

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