Applications of Cold Atmospheric Pressure Plasma in Dentistry- A Review

Mitthra, Suresh; Hemalatha, V.T.; Sundar, N. Mani; Nisha, Arthi

doi:10.9734/jpri/2022/v34i11a35533

Cited by 6 publications

(2 citation statements)

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“…Newer fields of interest include cancer therapy ( 12 , 13 ), dentistry ( 14 ) and ophthalmology ( 15 ). Many studies show that CAP can induce apoptosis of cancer cells in vitro ( 16–18 ).…”

Section: Introductionmentioning

confidence: 99%

Argon cold atmospheric plasma eradicates pathogens in vitro that are commonly associated with canine bacterial keratitis

Marx,

Oltmanns,

Meißner

et al. 2024

Front. Vet. Sci.

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PurposeTo investigate the antimicrobial effect of cold atmospheric plasma (CAP) on pathogens associated with canine bacterial keratitis.Materials and methodsPseudomonas aeruginosa, Staphylococcus pseudintermedius, and Streptococcus canis strains, which were obtained from dogs with infectious keratitis, were subjected to testing. For each species, four isolates and a reference strain were cultivated on Columbia sheep blood agar and treated with the kiNPen Vet® plasma pen from Neoplas GmbH, Greifswald, Germany. Various continuous treatment durations (0.5, 2, and 5 min) were applied, along with a 0.5-min treatment repeated four times at short intervals. These treatments were conducted at distances of 3 and 18 mm between the agar surface and the pen.ResultsCAP treatment reduced bacterial growth in all three species. The most effective treatment duration was 5 min at 3 mm distance, resulting in inhibition zones ranging from 19 to 22 mm for P. aeruginosa, 26–45 mm for S. pseudintermedius and an overall reduction of bacterial growth for Str. canis. Inhibition zones were smaller with decreasing treatment duration and larger distance. Treatment times of 30 s repeated four times and 2 min showed comparable results. Treatment with argon alone did not lead to visible reduction of bacterial growth.ConclusionArgon cold atmospheric plasma demonstrated a potent in vitro antimicrobial effect on P. aeruginosa, S. pseudintermedius and Str. canis strains with the latter showing the highest sensitivity.

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Section: Introductionmentioning

confidence: 99%

Argon cold atmospheric plasma eradicates pathogens in vitro that are commonly associated with canine bacterial keratitis

Marx,

Oltmanns,

Meißner

et al. 2024

Front. Vet. Sci.

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“…In the field of dentistry, plasma technology is recommended for controlling oral biofilms [4]. Plasma, a partially ionized gas with neutral atoms, molecules, ions, and electrons, exhibits strong antimicrobial capabilities, facilitating precise and targeted use.…”

Section: Introductionmentioning

confidence: 99%

Influence of Biofilm Maturity on the Antibacterial Efficacy of Cold Atmospheric Plasma in Oral Microcosm Biofilms

Kim

2024

Biomedicines

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As biofilms mature, biomass and extracellular polysaccharide (EPS) content increases, enhancing pathogenicity. Therefore, this study aimed to evaluate the antibacterial efficacy of cold atmospheric plasma (CAP) against oral microcosm biofilms and the influence of biofilm maturity on treatment. Oral microcosm biofilms were cultured on hydroxyapatite disks for 2 and 6 days. Based on the treatment and biofilm maturity, these were subsequently allocated into six groups (N = 19 each): Groups 1 and 2 were incubated with distilled water for 1 min; Groups 3 and 4 were treated with CAP for 2 min, and Groups 5 and 6 were treated with 0.12% chlorhexidine gluconate for 1 min. Groups 1, 3, and 5 represent 2-day biofilms, and Groups 2, 4, and 6 represent 6-day biofilms. Treatments were repeated daily for 5 days. Antibacterial efficacy was analyzed by measuring oral biofilms’ red fluorescence intensity (RatioR/G) and quantifying EPS content and bacterial viability. The RatioR/G was 1.089-fold and 1.104-fold higher in Groups 4 and 6 than in Groups 3 and 5 following antibacterial treatment, respectively (p < 0.001). EPS content increased by 1.71-fold in Group 6 than in Group 5 (p < 0.001). Bacterial survival rate was the lowest in Group 3 (p = 0.005). These findings underscore the relevance of CAP treatment in maintaining antibacterial efficacy regardless of the biofilm development stage, highlighting its potential utility in oral care.

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