Applications of Bacteriophage Cocktails to Reduce Salmonella Contamination in Poultry Farms

Evran, Şefika; Tayyarcan, Emine Kübra; Acar-Soykut, Esra; Boyaci, İsmail Hakkı

doi:10.1007/s12560-021-09501-0

Cited by 14 publications

(3 citation statements)

References 33 publications

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“…Research is ongoing to reduce on-farm pathogen occurrence in broilers for Salmonella [25,26,29,[51][52][53], Campylobacter [54][55][56][57][58], Escherichia coli [53,[59][60][61] or Clostridium perfringens [62][63][64], and in laying hens for Salmonella [65][66][67]. Other on-farm interventions included the control of bacteria in drinking water, shavings and on plastic surfaces [68]. Moreover, studies on post-harvest interventions using bacteriophages were conducted to control pathogen contamination in raw chicken meat against Salmonella [69,70] and Clostridium perfringens [71,72] in raw turkey meat [73] and eggs against Salmonella [74,75].…”

Section: Current Utilities and Legislations In The Use Of Phage In Po...mentioning

confidence: 99%

Phage-Based Biosanitation Strategies for Minimizing Persistent Salmonella and Campylobacter Bacteria in Poultry

Jordá,

Lorenzo-Rebenaque,

Montoro-Dasi

et al. 2023

Animals

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Control strategies to minimize pathogenic bacteria in food animal production are one of the key components in ensuring safer food for consumers. The most significant challenges confronting the food industry, particularly in the major poultry and swine sectors, are antibiotic resistance and resistance to cleaning and disinfection in zoonotic bacteria. In this context, bacteriophages have emerged as a promising tool for zoonotic bacteria control in the food industry, from animals and farm facilities to the final product. Phages are viruses that infect bacteria, with several advantages as a biocontrol agent such as high specificity, self-replication, self-limitation, continuous adaptation, low inherent toxicity and easy isolation. Their development as a biocontrol agent is of particular interest, as it would allow the application of a promising and even necessary “green” technology to combat pathogenic bacteria in the environment. However, bacteriophage applications have limitations, including selecting appropriate phages, legal restrictions, purification, dosage determination and bacterial resistance. Overcoming these limitations is crucial to enhance phage therapy’s effectiveness against zoonotic bacteria in poultry. Thus, this review aims to provide a comprehensive view of the phage-biosanitation strategies for minimizing persistent Salmonella and Campylobacter bacteria in poultry.

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Section: Current Utilities and Legislations In The Use Of Phage In Po...mentioning

confidence: 99%

Phage-Based Biosanitation Strategies for Minimizing Persistent Salmonella and Campylobacter Bacteria in Poultry

Jordá,

Lorenzo-Rebenaque,

Montoro-Dasi

et al. 2023

Animals

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“…A previous study used a phage cocktail (CNPSA1, CNPSA3, and CNPSA4) to treat broiler chickens infected with S. Enteritidis PT4; phage-treated chickens had a 3.5-fold reduction in Salmonella cecal count compared to the control group 8 . In addition, several studies have focused on phage oral administration in chickens to improve the treatment effectiveness 9 , 10 . In most cases, phages were added to animal feeds or drinking water to enable effective oral administration into the chicken GI tract to control bacterial pathogens.…”

Section: Introductionmentioning

confidence: 99%

Characterization of two novel Salmonella phages having biocontrol potential against Salmonella spp. in gastrointestinal conditions

Zhang,

Chu,

Liao

et al. 2024

Sci Rep

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Salmonella is a primary enteric pathogen related to the contamination of poultry and other food products in numerous foodborne outbreaks. The continuous emergence of multidrug-resistant bacteria has become a serious issue due to the overuse of antibiotics. Hence, lytic phages are considered alternative biocontrol agents against these bacterial superbugs. Here, two Salmonella phages—S4lw and D5lw—were subjected to genomic and biological characterization and further encapsulated to improve the stability under acidic conditions mimicking gastrointestinal conditions. The two lytic phages, S4lw and D5lw, taxonomically belong to new species under the Guernseyvirinae and Ackermannviridae families, respectively. Each phage showed antimicrobial activities against diverse Salmonella spp., such as S. Enteritidis and S. Typhimurium, achieving 1.7–3.4 log reduction after 2–6 h of treatment. The phage cocktail at a multiplicity of infection (MOI) of 100 or 1000 completely inhibited these Salmonella strains for at least 14 h at 25 °C. Additionally, the bead-encapsulated phage cocktail could withstand low pH and different simulated gut environments for at least 1 h. Overall, the newly isolated phages can potentially mitigate Salmonella spp. under the gastrointestinal environments through encapsulation and may be further applied via oral administration to resolve common antimicrobial resistance issues in the poultry production chain.

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“…To improve PCHS rapidity and specificity features, we considered the addition of lytic bacteriophages, based on their reported usage as environmental decontaminants [ 24 , 25 , 26 ]. Bacteriophages are procaryotic viruses characterized by a very narrow host range and a rapid action, and thanks to these features, have been suggested as effective agents for biological control against foodborne and plant pathogens, and for decontamination of industrial surfaces, farm facilities, and wastewater [ 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ]. Notably, they are also active against HAI-associated pathogens persistently contaminating hospital surfaces, including MDR bacteria such as Pseudomonas aeruginosa [ 34 ], and methicillin-resistant Staphylococcus aureus (MRSA) [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Potential Use of a Combined Bacteriophage–Probiotic Sanitation System to Control Microbial Contamination and AMR in Healthcare Settings: A Pre-Post Intervention Study

D’Accolti

Soffritti

Bini

et al. 2023

IJMS

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Microbial contamination in the hospital environment is a major concern for public health, since it significantly contributes to the onset of healthcare-associated infections (HAIs), which are further complicated by the alarming level of antimicrobial resistance (AMR) of HAI-associated pathogens. Chemical disinfection to control bioburden has a temporary effect and can favor the selection of resistant pathogens, as observed during the COVID-19 pandemic. Instead, probiotic-based sanitation (probiotic cleaning hygiene system, PCHS) was reported to stably abate pathogens, AMR, and HAIs. PCHS action is not rapid nor specific, being based on competitive exclusion, but the addition of lytic bacteriophages that quickly and specifically kill selected bacteria was shown to improve PCHS effectiveness. This study aimed to investigate the effect of such combined probiotic–phage sanitation (PCHSφ) in two Italian hospitals, targeting staphylococcal contamination. The results showed that PCHSφ could provide a significantly higher removal of staphylococci, including resistant strains, compared with disinfectants (−76%, p < 0.05) and PCHS alone (−50%, p < 0.05). Extraordinary sporadic chlorine disinfection appeared compatible with PCHSφ, while frequent routine chlorine usage inactivated the probiotic/phage components, preventing PCHSφ action. The collected data highlight the potential of a biological sanitation for better control of the infectious risk in healthcare facilities, without worsening pollution and AMR concerns.

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Applications of Bacteriophage Cocktails to Reduce Salmonella Contamination in Poultry Farms

Cited by 14 publications

References 33 publications

Phage-Based Biosanitation Strategies for Minimizing Persistent Salmonella and Campylobacter Bacteria in Poultry

Phage-Based Biosanitation Strategies for Minimizing Persistent Salmonella and Campylobacter Bacteria in Poultry

Characterization of two novel Salmonella phages having biocontrol potential against Salmonella spp. in gastrointestinal conditions

Potential Use of a Combined Bacteriophage–Probiotic Sanitation System to Control Microbial Contamination and AMR in Healthcare Settings: A Pre-Post Intervention Study

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