Applications of affinity chromatography in proteomics

Lee, Wen‐Chien; Lee, Kelvin H.

doi:10.1016/j.ab.2003.08.031

Cited by 164 publications

(78 citation statements)

References 75 publications

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“…As it has been shown that different breeding lines differ with respect to their susceptibility to A. pleuropneumoniae infection [22] animals of three different breeding lines were investigated. The glycoprotein isolation was carried out by lectin chromatography with Concanavalin A [29], which binds to carbohydrate structures containing glucose and mannose groups. These sugar residues can be found on chains of N-linked glycans but are absent on O-glycans, resulting in an exclusion of solely O-glycosylated proteins like the mucin-type glycoproteins of the airway epithelium [26].…”

Section: Discussionmentioning

confidence: 99%

Glycoprotein analysis of porcine bronchoalveolar lavage fluid reveals potential biomarkers corresponding to resistance toActinobacillus pleuropneumoniaeinfection

D¹,

Buettner²,

Naim³

et al. 2009

Vet. Res.

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-Biomarkers facilitating both pathogen-independent diagnosis of respiratory health and breeding selection of pigs with increased resistance to respiratory tract infections would be of considerable interest to the pig industry. Following this concept we performed a comparative glycoproteome analysis of bronchoalveolar lavage fluid (BALF) from healthy pigs and pigs 4 days (acute) and 20 days (chronic) after an experimental infection with Actinobacillus pleuropneumoniae. In order to identify possible differences in BALF glycoprotein patterns we investigated pigs of three different breeding lines (German Landrace, Piétrain, Hampshire). In total, 12 glycosylated proteins (alpha-1-acid glycoprotein, fetuin A, properdin, haptoglobin precursor, haptoglobin, hemoglobin, hyaluronidase, inter-alpha-trypsin inhibitor family heavy chain-related protein, alpha-1-antichymotrypsin 3, pulmonary surfactant-associated protein D (SP-D), transferrin, and alpha-1B-glycoprotein) were identified as being differentially expressed depending on the health status of the animal. Fetuin A levels were consistently low in chronically infected pigs thereby being a potential marker for chronic infection. Hyaluronidase levels were consistently high in all pigs after experimental infection independent on isolation of the pathogen thereby being a potential marker for previous pathogen contact and latent infection. High levels of fetuin A as well as low levels of haptoglobin and pulmonary SP-D correlated with the absence of lung lesions in pigs of the Hampshire breeding line, implying a potential application as selection markers for breeding programmes.porcine respiratory disease / glycoprotein analysis / bronchoalveolar lavage fluid / biomarker

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Section: Discussionmentioning

confidence: 99%

Glycoprotein analysis of porcine bronchoalveolar lavage fluid reveals potential biomarkers corresponding to resistance toActinobacillus pleuropneumoniaeinfection

D¹,

Buettner²,

Naim³

et al. 2009

Vet. Res.

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“…In this context, the immobilization of ligands plays a central role either for bioseparation and concentration of biomolecules (Lee & Lee, 2004;Azarkan et al, 2007), for pull-down assays and mass spectrometry analysis (Bécamel et al, 2002) or for highthroughput screening in array format (Cahill, 2000). However, examples of vinyl sulfone functionalized supports either for affinity chromatography or arrays are scarce.…”

Section: Vinyl Sulfone-based Affinity Chromatography Applicationsmentioning

confidence: 99%

Vinyl Sulfone: A Multi-Purpose Function in Proteomics

Lopéz-Jaramillo¹,

Hernández-Matéo²,

Santoyo‐González³

2012

Integrative Proteomics

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“…Subsequent electrospray ionization-MS screening for the occurrence of peptides containing N-terminal pyroglutamate residues was performed to identify the initial lipid-modified glutamic acid residues (348). For a general overview and detailed description of new, highly sensitive MS methods, as well as affinity chromatography-based posttranslational modification trapping methods (including those used for protein glycosylation and glycation analysis), the reader is referred to excellent reviews on this topic (192,193,220,225,262,265,290,441).…”

Section: Pargsmentioning

confidence: 99%

Nuclear ADP-Ribosylation Reactions in Mammalian Cells: Where Are We Today and Where Are We Going?

Hassa

Haenni

Elser

et al. 2006

Microbiol Mol Biol Rev

629

644

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SUMMARY Since poly-ADP ribose was discovered over 40 years ago, there has been significant progress in research into the biology of mono- and poly-ADP-ribosylation reactions. During the last decade, it became clear that ADP-ribosylation reactions play important roles in a wide range of physiological and pathophysiological processes, including inter- and intracellular signaling, transcriptional regulation, DNA repair pathways and maintenance of genomic stability, telomere dynamics, cell differentiation and proliferation, and necrosis and apoptosis. ADP-ribosylation reactions are phylogenetically ancient and can be classified into four major groups: mono-ADP-ribosylation, poly-ADP-ribosylation, ADP-ribose cyclization, and formation of O-acetyl-ADP-ribose. In the human genome, more than 30 different genes coding for enzymes associated with distinct ADP-ribosylation activities have been identified. This review highlights the recent advances in the rapidly growing field of nuclear mono-ADP-ribosylation and poly-ADP-ribosylation reactions and the distinct ADP-ribosylating enzyme families involved in these processes, including the proposed family of novel poly-ADP-ribose polymerase-like mono-ADP-ribose transferases and the potential mono-ADP-ribosylation activities of the sirtuin family of NAD+-dependent histone deacetylases. A special focus is placed on the known roles of distinct mono- and poly-ADP-ribosylation reactions in physiological processes, such as mitosis, cellular differentiation and proliferation, telomere dynamics, and aging, as well as “programmed necrosis” (i.e., high-mobility-group protein B1 release) and apoptosis (i.e., apoptosis-inducing factor shuttling). The proposed molecular mechanisms involved in these processes, such as signaling, chromatin modification (i.e., “histone code”), and remodeling of chromatin structure (i.e., DNA damage response, transcriptional regulation, and insulator function), are described. A potential cross talk between nuclear ADP-ribosylation processes and other NAD+-dependent pathways is discussed.

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Applications of affinity chromatography in proteomics

Cited by 164 publications

References 75 publications

Glycoprotein analysis of porcine bronchoalveolar lavage fluid reveals potential biomarkers corresponding to resistance toActinobacillus pleuropneumoniaeinfection

Glycoprotein analysis of porcine bronchoalveolar lavage fluid reveals potential biomarkers corresponding to resistance toActinobacillus pleuropneumoniaeinfection

Vinyl Sulfone: A Multi-Purpose Function in Proteomics

Nuclear ADP-Ribosylation Reactions in Mammalian Cells: Where Are We Today and Where Are We Going?

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