Applications of a high maltose forming, thermo-stable α-amylase from an extremely alkalophilic Bacillus licheniformis strain AS08E in food and laundry detergent industries

Roy, Jetendra Kumar; Mukherjee, Ashis K.

doi:10.1016/j.bej.2013.06.012

Cited by 49 publications

(68 citation statements)

References 30 publications

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“…Production of the higher concentration of amylase under the influence of temperature in acidic as well as alkaline conditions has been reported [23,24]. The pH optima, in the present study, for enzyme activity and stability was similar as described by Asoodeh et al [25].…”

Section: Enzyme Activity and Stabilitysupporting

confidence: 67%

Production of Industrially Important Enzymes by Thermobacilli Isolated From Hot Springs of India

Dhyani

Gururani

Selim

et al. 2017

RIB

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Enzymes from thermophilic bacteria have received great attention for their potential applications in various industrial sectors. The present study deals with the production of five thermozymes (amylase, lipase, xylanase, protease and cellulase) from 10 thermophilic bacterial species, originally isolated from two hot springs namely Soldhar and Ringigad in Uttarakhand Himalaya, India. The bacterial isolate GBPI_25 produced maximum amylase (1217.86 U/ml) at 45 °C and 5 pH, GBPI 3 produced maximum lipase (22.59 U/ml) at 65 °C and 9 pH, GBPI_25 produced maximum xylanase (98.07 U/ml) at45 °C and 9 pH, GBPI_35 produced maximum protease (16.66 U/ml) at 55 °C and 9 pH, and GBPI 4 produced maximum cellulose (108.68 U/ml) at 45 °C and 5 pH. Crude enzyme preparations showed thermal and pH activities at broad temperature and pH range between 10-100 °C and 3-11 pH, respectively, with different temperature and pH optima. Amylase, xylanase and cellulase showed maximum activity at 50 °C while lipase and protease showed higher activity at 40 and 60 °C, respectively. Enzyme activity at wide temperature range-cellulase and protease from 10-100 °C, amylase and xylanasefrom10-90 °C, and lipase activity from 10-80 °C were the remarkable records from this study. Similarly, pH range for amylase and lipase activity was recorded from 4-11, for xylanase from 3-9, and for protease and cellulase from 3-10. All the thermozymes showed maximum stability at 40 °C and pH 5 except cellulase that showed higher stability at40 °C and neutral pH.

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Section: Enzyme Activity and Stabilitysupporting

confidence: 67%

Production of Industrially Important Enzymes by Thermobacilli Isolated From Hot Springs of India

Dhyani

Gururani

Selim

et al. 2017

RIB

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“…-Amylases (1,4-␣-D-glucan glucanohydrolase; EC 3.2.1.1) constitute a class of industrial enzymes that constitute approximately 25% of the world enzyme market and are used in the industrial production of starches, detergents, textiles, and pharmaceuticals (1). Among the ␣-amylases, alkaline ␣-amylases are of special interest because they work under alkaline conditions encountered in the starch, detergent, and textile industries (2,3).…”

mentioning

confidence: 99%

In Silico Rational Design and Systems Engineering of Disulfide Bridges in the Catalytic Domain of an Alkaline α-Amylase from Alkalimonas amylolytica To Improve Thermostability

Liu

Deng

Yang

et al. 2014

Appl Environ Microbiol

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High thermostability is required for alkaline ␣-amylases to maintain high catalytic activity under the harsh conditions used in textile production. In this study, we attempted to improve the thermostability of an alkaline ␣-amylase from Alkalimonas amylolytica through in silico rational design and systems engineering of disulfide bridges in the catalytic domain. Specifically, 7 residue pairs (P35-G426, Q107-G167, G116-Q120, A147-W160, G233-V265, A332-G370, and R436-M480) were chosen as engineering targets for disulfide bridge formation, and the respective residues were replaced with cysteines. Three single disulfide bridge mutants-P35C-G426C, G116C-Q120C, and R436C-M480C-of the 7 showed significantly enhanced thermostability. Combinational mutations were subsequently assessed, and the triple mutant P35C-G426C/G116C-Q120C/R436C-M480C showed a 6-fold increase in half-life at 60°C and a 5.2°C increase in melting temperature compared with the wild-type enzyme. Interestingly, other biochemical properties of this mutant also improved: the optimum temperature increased from 50°C to 55°C, the optimum pH shifted from 9.5 to 10.0, the stable pH range extended from 7.0 to 11.0 to 6.0 to 12.0, and the catalytic efficiency (k cat /K m ) increased from 1.8 ؋ 10 4 to 2.4 ؋ 10 4 liters/g · min. The possible mechanism responsible for these improvements was explored through comparative analysis of the model structures of wild-type and mutant enzymes. The disulfide bridge engineering strategy used in this work may be applied to improve the thermostability of other industrial enzymes.

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“…[28], Saccharopolyspora sp. [25], and Streptomyces sp. [26] are also used for detergentcompatible amylases production ( Table 2).…”

Section: Production Of Bacterial Detergent-compatible Amylasesmentioning

confidence: 99%

“…A3-15 Compost soil Starch agar Arikan [18] Saccharopolyspora sp. A9 Marine sediment Starch agar Chakraborty et al [25] Bacillus licheniformis AS08E Soil Starch agar Roy and Mukherjee [24] Streptomyces strain A3 Marine sediment Starch agar Chakraborty et al [26] Bacillus subtilis AS-SO1a Soil Starch agar Roy et al [9] Alicyclobacillus acidocaldarius Water effluent Starch agar Satheeshkumar et al [27] Bacillus sp. strain TSCVKK Soil Starch agar Kiran and Chandra [21] Nesterenkonia sp.…”

Section: Microorganismmentioning

confidence: 99%

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Detergent-Compatible Bacterial Amylases

Niyonzima

2014

Appl Biochem Biotechnol

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Proteases, lipases, amylases, and cellulases are enzymes used in detergent formulation to improve the detergency. The amylases are specifically supplemented to the detergent to digest starchy stains. Most of the solid and liquid detergents that are currently manufactured contain alkaline enzymes. The advantages of using alkaline enzymes in the detergent formulation are that they aid in removing tough stains and the process is environmentally friendly since they reduce the use of toxic detergent ingredients. Amylases active at low temperature are preferred as the energy consumption gets reduced, and the whole process becomes cost-effective. Most microbial alkaline amylases are used as detergent ingredients. Various reviews report on the production, purification, characterization, and application of amylases in different industry sectors, but there is no specific review on bacterial or fungal alkaline amylases or detergent-compatible amylases. In this mini-review, an overview on the production and property studies of the detergent bacterial amylases is given, and the stability and compatibility of the alkaline bacterial amylases in the presence of the detergents and the detergent components are highlighted.

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Applications of a high maltose forming, thermo-stable α-amylase from an extremely alkalophilic Bacillus licheniformis strain AS08E in food and laundry detergent industries

Cited by 49 publications

References 30 publications

Production of Industrially Important Enzymes by Thermobacilli Isolated From Hot Springs of India

Production of Industrially Important Enzymes by Thermobacilli Isolated From Hot Springs of India

In Silico Rational Design and Systems Engineering of Disulfide Bridges in the Catalytic Domain of an Alkaline α-Amylase from Alkalimonas amylolytica To Improve Thermostability

Detergent-Compatible Bacterial Amylases

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