Applications and performance of a MALDI-ToF mass spectrometer with quadratic field reflectron technology

Flensburg, John; Haid, Daniel; Blomberg, Johan; Bielawski, Jacek; Ivansson, Daniel

doi:10.1016/j.jbbm.2004.01.010

Cited by 15 publications

(8 citation statements)

References 21 publications

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“…The equipment and proce- Fig. 2 dure used enables to measure molecular weights of proteins with an error up to 0.2 % [15]. Peaks of weights to charge ratio higher than 14 173 Da (calculated weight of non-reduced α-LA with 1 H + cation is 14 179 Da) may be identified as the products of protein aggregation occurring during measurement [15].…”

Section: Resultsmentioning

confidence: 99%

Protein homogeneity testing by reversed-phase high-performance liquid chromatography of reduced and non-reduced samples

2005

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Protein homogeneity testing by reversed-phase high-performance liquid chromatography of reduced and non-reduced samples RAPID COMMUNICATION Summary-The concept of this study was based on the assumption that protein standards obtained using the same method may reveal heterogeneity explainable by changes in the disulphide bonding pattern. Two lots of bovine α-lactalbumin standard were subjected to reversed-phase high-performance liquid chromatography (RP-HPLC). One of them was homogenous. The other revealed the presence of two major fractions when non reduced. Neither RP-HPLC after protein reduction nor mass spectrometry showed differences between them. This indicates that one of the lots contained isomers with different locations of disulphide bonds. A comparison of RP-HPLC profiles obtained with and without reduction may be thus recommended as a tool for testing homogeneity of protein standards, especially those used in experiments involving denaturation.

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Section: Resultsmentioning

confidence: 99%

Protein homogeneity testing by reversed-phase high-performance liquid chromatography of reduced and non-reduced samples

2005

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“…Vários desenvolvimentos conduziram a refletores modernos com elevado desempenho [9][10][11] , com considerável redução no tamanho acelerados na direção do eixo z e ejetados pelo eletrodo end cap de saída dos íons sendo, então, detectados. As equações que descrevem o movimento dos íons do ion trap são similares às descritas para o quadrupolo linear.…”

Section: Analisadores De Massas Do Tipo Ion Trap (Aprisionadores De íunclassified

A cromatografia líquida moderna e a espectrometria de massas: Finalmente "compatíveis"? II. A escolha do analisador de massas

Lanças¹

2013

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A cromatografia líquida de alta eficiência (HPLC ou CLAE) é uma das principais técnicas utilizadas na análise de compostos não voláteis e/ou termicamente instáveis. Apesar de ser uma excelente técnica de separação, a HPLC necessita de uma técnica confirmatória quando a análise qualitativa (confirmação da identidade química) é também necessária. Dentre as várias opções existentes no momento, a espectrometria de massas (ms) é a técnica que melhor fornece as informações estruturais necessárias. O acoplamento entre estas duas técnicas dá origem a uma ferramenta analítica versátil e de grande potencial na análise qualitativa e quantitativa: a LC/MS (cromatografia líquida acoplada à espectrometria de massas). O presente artigo, segundo de uma série sobre o tema, apresenta de forma crítica e detalhada a escolha do analisar de massas para LC-MS(MS).

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“…Sequence information on peptides can be generated in MALDI-TOF-MS by taking advantage of this PSD as described in [4]. Recent instrumental developments provide for levels of sensitivity and accuracy that make these techniques major analytical tools for proteome analysis [60,61].…”

Section: Protein Identification and Structural Characterizationmentioning

confidence: 99%

Surface enhanced laser desorptions ionization-time of flight-mass spectrometry analysis in complex food and biological systems

Rawel

Rohn

Kröll

et al. 2005

Mol. Nutr. Food Res.

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Applications and performance of a MALDI-ToF mass spectrometer with quadratic field reflectron technology

Cited by 15 publications

References 21 publications

Protein homogeneity testing by reversed-phase high-performance liquid chromatography of reduced and non-reduced samples

Protein homogeneity testing by reversed-phase high-performance liquid chromatography of reduced and non-reduced samples

A cromatografia líquida moderna e a espectrometria de massas: Finalmente "compatíveis"? II. A escolha do analisador de massas

Surface enhanced laser desorptions ionization-time of flight-mass spectrometry analysis in complex food and biological systems

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