Applications and Benefits of Thermophilic Microorganisms and Their Enzymes for Industrial Biotechnology

Gomes, Eleni; Souza, Angélica Rodrigues de; Orjuela, Guillermo Ladino; Silva, Roberto da; Oliveira, Tássio Brito de; Rodrigues, André

doi:10.1007/978-3-319-27951-0_21

Cited by 42 publications

(26 citation statements)

References 199 publications

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“…In order to survive such high temperatures, thermophilic organisms are often spore-forming and possess thermally stable enzymes that allow normal functioning of cellular processes under such extreme conditions. Examples of such enzymes and their characteristics and functionalities are extensively described in the literature (e.g., Demirjian et al, 2001;Vieille and Zeikus, 2001;Gomes et al, 2016). P. aeruginosa incubated at 37 • C produces rhamnolipids, which at a concentration of 1 mg/ml this biosurfactant has been shown to recover 22% of oil from sand-packed columns at 40 • C, and to be more effective than the chemical surfactant Petrostep (15.6% oil recovery) (Gudiña et al, 2015).…”

Section: Thermo-tolerant Microorganismsmentioning

confidence: 99%

Use of Microorganisms in the Recovery of Oil From Recalcitrant Oil Reservoirs: Current State of Knowledge, Technological Advances and Future Perspectives

2020

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The depletion of oil resources, increasing global energy demand, the current low, yet unpredictable, price of oil, and increasing maturity of major oil fields has driven the need for the development of oil recovery technologies that are less costly and, where possible, environmentally compatible. Using current technologies, between 20 and 40% of the original oil in a reservoir can be extracted by conventional production operations (e.g., vertical drilling), with secondary recovery methods yielding a further 15-25%. Hence, up to 55% of the original oil can remain unrecovered in a reservoir. Enhanced oil recovery (EOR) is a tertiary recovery process that involves application of different thermal, chemical, and microbial processes to recover an additional 7-15% of the original oil in place (OOIP) at an economically feasible production rate from poor-performing and depleted oil wells. EOR can significantly impact oil production, as increase in the recovery rate of oil by even a small margin could bring significant revenues without developing unconventional resources. Microbial enhanced oil recovery (MEOR) is an attractive, alternative oil recovery approach, which is claimed to potentially recover up to 50% of residual oil. The in situ production of biological surface-active compounds (e.g., biosurfactants) during the MEOR process does not require vast energy inputs and are not affected by global crude oil prices. Compared to other EOR methods, MEOR can be an economically and more environmentally friendly alternative. In this review, the current state of knowledge of MEOR, with insights from discussions with the industry and other stakeholders, is presented and in addition to the future outlook for this technology.

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Section: Thermo-tolerant Microorganismsmentioning

confidence: 99%

Use of Microorganisms in the Recovery of Oil From Recalcitrant Oil Reservoirs: Current State of Knowledge, Technological Advances and Future Perspectives

2020

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“…Thermophilic bacteria can potentially be used as platforms for efficient functional screening of thermostable enzymes at elevated temperatures [1,3]. The benefits of utilizing thermophilic hosts in bioprocesses include decreased risk of contamination of fermentation cultures and lower cooling costs compared to mesophilic hosts [10,11]. It has also been suggested that some thermostable enzymes need high temperatures (and consequently thermophilic hosts) for proper expression and folding [12].…”

Section: Thermophilic Bacteria As Hosts For Recombinant Protein Produmentioning

confidence: 99%

Establishment of a functional system for recombinant production of secreted proteins at 50 °C in the thermophilic Bacillus methanolicus

et al. 2020

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Background: The suitability of bacteria as microbial cell factories is dependent on several factors such as price of feedstock, product range, production yield and ease of downstream processing. The facultative methylotroph Bacillus methanolicus is gaining interest as a thermophilic cell factory for production of value-added products from methanol. The aim of this study was to expand the capabilities of B. methanolicus as a microbial cell factory by establishing a system for secretion of recombinant proteins. Results: Native and heterologous signal peptides were tested for secretion of α-amylases and proteases, and we have established the use of the thermostable superfolder green fluorescent protein (sfGFP) as a valuable reporter protein in B. methanolicus. We demonstrated functional production and secretion of recombinant proteases, α-amylases and sfGFP in B. methanolicus MGA3 at 50 °C and showed that the choice of signal peptide for optimal secretion efficiency varies between proteins. In addition, we showed that heterologous production and secretion of α-amylase from Geobacillus stearothermophilus enables B. methanolicus to grow in minimal medium with starch as the sole carbon source. An in silico signal peptide library consisting of 169 predicted peptides from B. methanolicus was generated and will be useful for future studies, but was not experimentally investigated any further here. Conclusion: A functional system for recombinant production of secreted proteins at 50 °C has been established in the thermophilic B. methanolicus. In addition, an in silico signal peptide library has been generated, that together with the tools and knowledge presented in this work will be useful for further development of B. methanolicus as a host for recombinant protein production and secretion at 50 °C.

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“…It was found that prokaryotic (Bacteria and Archaea) can grow in much higher temperature (113 • C ) than eukaryotic (not over 60 • C ) [9].Thermophilic bacteria such asThermoanaerobacteriales, Bacillales, and Clostridiales were isolated from a variety of environmental and also ordered as alkaliphiles, neutrophils and acidophiles; strict anaerobes, selective anaerobes, and aerobes; and chemolithotrophs and chemoorganotrophs [10].…”

Section: High Temperaturementioning

confidence: 99%

Extremophile Current Challenges and New Gate of Knowledge by Nanoparticles Pathways.

Moayad¹,

Zha²,

Yan³

2017

IOSRJPBS

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Applications and Benefits of Thermophilic Microorganisms and Their Enzymes for Industrial Biotechnology

Cited by 42 publications

References 199 publications

Use of Microorganisms in the Recovery of Oil From Recalcitrant Oil Reservoirs: Current State of Knowledge, Technological Advances and Future Perspectives

Use of Microorganisms in the Recovery of Oil From Recalcitrant Oil Reservoirs: Current State of Knowledge, Technological Advances and Future Perspectives

Establishment of a functional system for recombinant production of secreted proteins at 50 °C in the thermophilic Bacillus methanolicus

Extremophile Current Challenges and New Gate of Knowledge by Nanoparticles Pathways.

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