Application values of miR-194 and miR-29 in the diagnosis and prognosis of gastric cancer

Hou, Yunxia; Tuo, Zhongzhen; Wei, Fangmeng

doi:10.3892/etm.2018.5931

Cited by 18 publications

(18 citation statements)

References 29 publications

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“…Our results are in accordance with many other studies (Tanaka et al, 2009;Elhamamsy et al, 2017) who reported that mir-92a plasma levels were significantly lower in AML patients and also it was found to be very low in tumor tissues retrieved from patients with gastric cancer (Zhao et al, 2018), However, other studies found that suppression of miR-92a will induce apoptosis and can be considered as a novel method for treatment of Acute Promyelocytic Leukemia (Sharifi et al, 2014). Also, upregulation in miR-92a was observed in colorectal cancer (Elshafei et al, 2017) and cervical cancer (Zhou et al, 2015;Kawai et al, 2018).…”

Section: Discussionsupporting

confidence: 93%

Assessment of the Diagnostic Potential of miR-29a-3p and miR-92a-3p as Circulatory Biomarkers in Acute Myeloid Leukemia

Gado

Mousa

Badawy

et al. 2019

Asian Pac J Cancer Prev

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Section: Discussionsupporting

confidence: 93%

Assessment of the Diagnostic Potential of miR-29a-3p and miR-92a-3p as Circulatory Biomarkers in Acute Myeloid Leukemia

Gado

Mousa

Badawy

et al. 2019

Asian Pac J Cancer Prev

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“…In the context of miR-29a-5p, lower expression levels in FHWT compared to the control group were shown, whereas in metastatic comparison, these miRNA expression levels were evidently lower compared to the control group as well. The results obtained together showed that miR-29a decreases in tumor tissues compared to healthy kidney tissue; it also decreased in tumors with favorable histology and metastatic tumors, consistent with the gastric cancer data reported by Zhao et al in 2018 ( 22 ). In metastatic prostate cancer, its expression also diminished and it was demonstrated that it participates in cell proliferation inhibition ( 23 ).…”

Section: Discussionsupporting

confidence: 90%

MicroRNA Profiling in Wilms Tumor: Identification of Potential Biomarkers

et al. 2020

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Wilms tumor (WT) is the most frequently diagnosed malignant renal tumor in children. With current treatments, ∼90% of children diagnosed with WT survive and generally present with tumors characterized by favorable histology (FHWT), whereas prognosis is poor for the remaining 10% of cases where the tumors are characterized by cellular diffuse anaplasia (DAWT). Relatively few studies have investigated microRNA-related epigenetic regulation and its relationship with altered gene expression in WT. Here, we aim to identify microRNAs differentially expressed in WT and describe their expression in terms of cellular anaplasia, metastasis, and association with the main genetic alterations in WT to identify potential prognostic biomarkers. Expression profiling using TaqMan low-density array was performed in a discovery cohort consisting of four DAWT and eight FHWT samples. Relative quantification resulted in the identification of 109 (48.7%) microRNAs differentially expressed in both WT types. Of these, miR-10a-5p, miR-29a-3p, miR-181a-5p, miR-200b-3p, and miR-218-5p were selected and tested by RT-qPCR on a validation cohort of 53 patient samples. MiR-29a and miR-218 showed significant differences in FHWT with low (P = 0.0018) and high (P = 0.0131) expression, respectively. To discriminate between miRNA expression FHWTs and healthy controls, the receiver operating characteristic (ROC) curves were obtained; miR-29a AUC was 0.7843. Furthermore, low expression levels of miR-29a and miR-200b (P = 0.0027 and P = 0.0248) were observed in metastatic tumors. ROC curves for miR-29a discriminated metastatic patients (AUC = 0.8529) and miR-200b (AUC = 0.7757). To confirm the differences between cases with poor prognosis, we performed in situ hybridization for three microRNAs in five DAWT and 17 FHWT samples, and only significant differences between adjacent tissues and FHWT tumors were found for miR-181a, miR-200b, and miR-218, in both total pixels and nuclear analyses. Analysis of copy number variation in genes showed that the most prevalent alterations were WTX (47%), IGF2 (21%), 1q (36%) gain, 1p36 (16%), and WTX deletion/1q duplicate (26%). The five microRNAs evaluated are involved in the Hippo signaling pathway and participate in Wilms tumor development through their effects on differentiation, proliferation, angiogenesis, and metastasis.

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“…The miR-29 family shares a seed sequence with miR-BART1-3p. While most studies have suggested a tumor suppressor function of miR-29 with down-regulated expression in multiple human carcinoma including GC, some reports showed oncogenic functions of the miR-29 family [36][37][38]. Whether dysregulation of the miR-29 family sharing a seed sequence with miR-BART1-3p can be responsible for DAB2 regulation, and whether coordinated action between miR-BART1-3p and miR-29 affects viral life cycle, are questions that warrant further investigation.…”

Section: Discussionmentioning

confidence: 99%

Epstein-Barr virus miR-BART1-3p suppresses apoptosis and promotes migration of gastric carcinoma cells by targeting DAB2

2020

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Although Epstein-Barr virus (EBV) is known to encode over 40 different miRNAs of its own, the roles of most EBV miRNAs remain unknown. Disabled homolog 2 (DAB2) is a putative tumor suppressor, but its role in gastric carcinoma (GC), especially in EBV-associated GC, needs to be clarified. Our qRT-PCR and mRNA microarray results showed that DAB2 expression was down-regulated in EBV-positive GC cells compared to EBV-negative cells. Four BART miRNAs that might target DAB2 were predicted, and we found, using a luciferase reporter assay, that miR-BART1-3p directly targeted the 3'-UTR of DAB2. The miR-BART1-3p transfection decreased DAB2 expression at both mRNA and protein levels, while transfection of an inhibitor of miR-BART1-3p, miR-BART1-3p(i), increased DAB2 expression. In addition, miR-BART1-3p as well as siDAB2 increased migration and decreased apoptosis. Meanwhile, miR-BART1-3p(i) or pcDNA3.1-DAB2 transfection decreased migration and increased apoptosis in EBV-infected GC cells. Furthermore, decreased migration by miR-BART1-3p(i) was abrogated by co-transfected siDAB2, while decreased migration by miR-BART1-3p(i) was further suppressed by a co-transfected DAB2 over-expression vector. Our data suggest that miR-BART1-3p plays an important role in the tumorigenesis of EBV-associated GC by directly targeting DAB2.

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Application values of miR-194 and miR-29 in the diagnosis and prognosis of gastric cancer

Cited by 18 publications

References 29 publications

Assessment of the Diagnostic Potential of miR-29a-3p and miR-92a-3p as Circulatory Biomarkers in Acute Myeloid Leukemia

Assessment of the Diagnostic Potential of miR-29a-3p and miR-92a-3p as Circulatory Biomarkers in Acute Myeloid Leukemia

MicroRNA Profiling in Wilms Tumor: Identification of Potential Biomarkers

Epstein-Barr virus miR-BART1-3p suppresses apoptosis and promotes migration of gastric carcinoma cells by targeting DAB2

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