Application to Photocatalytic H₂ Production of a Whole‐Cell Reaction by Recombinant Escherichia coli Cells Expressing [FeFe]‐Hydrogenase and Maturases Genes

Abstract

Ap hotocatalytic H 2 production system using an inorganic-bio hybrid photocatalyst could contribute to the efficient utilization of solar energy,b ut would require the development of an ew approach for preparing aH 2 -forming biocatalyst. In the present study,weconstructed arecombinant strain of Escherichia coli expressing the genes encoding the [FeFe]-hydrogenase and relevant maturases from Clostridium acetobutylicum NBRC 13948 for use as ab iocatalyst. We investigated the direct application of aw hole-cell of the recombinant E. coli. The combination of TiO 2 ,m ethylviologen, and the recombinant E. coli formed H 2 under light irradiation, demonstrating that whole cells of the recombinant E. coli could be employed

Visible‐Light Direct Conversion of Ethanol to 1,1‐Diethoxyethane and Hydrogen over a Non‐Precious Metal Photocatalyst

“…[11][12][13][14][15] However,asacrificial agent (e.g.,m ethanol and ethanol) needs to be added to improvet he efficiency of solar-to-H 2 conversion in most reactions ystems. [16][17][18][19][20][21][22] The main function of theses acrificial agents is capturing photogenerated holes to accelerate water oxidationk inetics. Unfortunately,m osto ft he sacrificial agent is oxidized to CO or CO 2 ,w hich is harmful to the environment.…”

A Periplasmic Photosensitized Biohybrid System for Solar Hydrogen Production

“…The result showed no clear evidence of a decline in SW bacteria number within 36 h. As prolonging the irradiation time to 48 h, the bacteria were almost dead (Figure S8, Supporting Information), which was mainly caused by the oxidation of holes and the lack of energy source that maintained bacteria cell metabolism. In this case, the bacterial viability maintained for a long time might be attributed to the good biocompatibility of QDs as well as the low damage of the visible light source, resulting in the sustained hydrogen production capacity of QDs/SW PPBS, where the previously reported whole-cell hybrid system cannot maintain H 2 production for more than 20 h. [17,18] These results indicated that the QDs/SW PPBS is capable of stable and efficient solar H 2 production.…”

“…To date, two classes of whole-cell biohybrid systems were pursued, in the form of the extracellular photosensitized biohybrid system and the cytoplasmic photosensitized biohybrid system. [17][18][19][20][21][22][23] In the extracellular photosensitized biohybrid system (Figure S1a, Supporting Information), photoexcited electrons from extracellular photosensitizers were delivered to the hydrogenase in the cell through diffusional redox mediators or membrane-bound redox-active proteins, further realizing the solar hydrogen production. Honda and coworkers pioneered the fabrication of the TiO 2 /Escherichia coli extracellular photosensitized biohybrid system, which showed boosted photocatalytic H 2 production.…”

“…Honda and coworkers pioneered the fabrication of the TiO 2 /Escherichia coli extracellular photosensitized biohybrid system, which showed boosted photocatalytic H 2 production. [17] However, the extracellular photosensitized biohybrid system suffers from the sluggish electron transfer owing to the slow transmembrane process. To address this issue, a cytoplasmic photosensitized biohybrid system had been developed by implanting ultrasmall Au nanoclusters into the cytoplasm of Moorella thermoacetica and therefore realized durable solar-driven CO 2 fixation.…”

Heterologous Hydrogenase Overproduction Systems for Biotechnology—An Overview

“…The presence of proteorhodopsin and retinal increased the hydrogen production~1.3-fold (4.25 µmol H 2 /(h•mg)) compared to the hydrogenase only strain [192]. On the other hand, the use of bioinorganic hybrid systems, comprised of a semiconductor and hydrogenase-producing bacterial cells, can be used [193]. This strategy was successfully applied to engineer E. coli cells that synthesizes a metal ion complex-binding protein on their surface that collects the light energy in addition to a hydrogenase that uses the solar energy for hydrogen production [15].…”