Objective. To examine the concentration of glucosamine in the synovial fluid and its pharmacokinetics in serum in a large animal model following dosing with glucosamine HCl at clinically relevant levels.Methods. Eight adult female horses were studied. After an overnight fast, glucosamine HCl (20 mg/kg of body weight) was administered by either nasogastric (NG) intubation or intravenous (IV) injection. Blood samples were collected before dosing and at 5, 15, 30, 60, 120, 180, 240, 360, 480, and 720 minutes after dosing. Synovial fluid samples were collected from the radiocarpal joints 48 hours before dosing and at 1 and 12 hours after dosing. Glucosamine was assayed by fluorophoreassisted carbohydrate electrophoresis.Results. The maximum concentration of glucosamine in serum reached ϳ300 M (ϳ50 g/ml) following IV dosing and ϳ6 M (ϳ1 g/ml) following NG dosing. Synovial fluid concentrations reached 9-15 M with IV dosing and 0.3-0.7 M with NG dosing, and remained elevated (range 0.1-0.7 M) in most animals even at 12 hours after dosing. Following NG dosing, the median serum maximal concentration of 6.1 M (range 4.38-7.58) was attained between 30 minutes and 4 hours postdose. The mean apparent volume of distribution was 15.4 liters/kg, the mean bioavailability was 5.9%, and the mean elimination half-life was 2.82 hours.Conclusion. Clinically relevant dosing of glucosamine HCl in this large monogastric animal model results in serum and synovial fluid concentrations that are at least 500-fold lower than those reported to modify chondrocyte anabolic and catabolic activities in tissue and cell culture experiments. We conclude that the apparent therapeutic benefit of dietary glucosamine on pain and joint space width in humans and animals may be secondary to its effects on nonarticular tissues, such as the intestinal lining, liver, or kidney, since these may be exposed to much high levels of glucosamine following ingestion.Glucosamine is now widely taken in the US as a dietary supplement (recommended dosage 20 mg/kg/ day) to relieve the discomfort of osteoarthritis (OA)-related joint pain. In Europe, a patented formulation of glucosamine (glucosamine sulfate) is a prescription drug, which is also widely reported to achieve cartilageprotective effects in knee OA (1,2). A quality assessment and meta-analysis of 6 placebo-controlled clinical trials using both glucosamine HCl (2 studies) and glucosamine sulfate (4 studies) concluded that some degree of efficacy appears probable in treating symptoms of knee OA (3). More recently, 2 meta-analyses of a large number of randomized control trials with glucosamine sulfate consistently concluded that the drug is both safe and effective for the treatment of symptomatic OA (4,5).