eCM 2002
DOI: 10.22203/ecm.v003a01
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Application of X-ray microanalysis to the study of cell physiology in cells attached to biomaterials

Abstract: X-ray microanalysis is commonly applied in biomaterials research to study changes in biomaterial composition, calcifications, or to identify particulates in tissue that has been in contact with biomaterials. Studies where the effect of biomaterials on the naturally occurring elements in the cells are studied are rare. Exposure to or contact with biomaterials may give rise to several cellular reactions with characteristic changes in elemental patterns. Experimental systems in which cultured cells are exposed to… Show more

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Cited by 12 publications
(18 citation statements)
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References 34 publications
(32 reference statements)
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“…Moreover, high concentrations of sulfur and phosphorous and moderate concentrations of magnesium and calcium were also found at P6. From a microanalytical point of view, phosphorus is an element that is associated with cell mass and organic constituents, nucleic acids contents and the level of cellular phosphorylation, and cells characterized by a severe structural damage show a decrease in intracellular concentrations of phosphorus [21]. Therefore, the high values found at P6 would confirm the vital status of these cells, whereas the decrease at P7 could be related to a possible structural damage of these cells.…”
Section: Discussionmentioning
confidence: 99%
“…Moreover, high concentrations of sulfur and phosphorous and moderate concentrations of magnesium and calcium were also found at P6. From a microanalytical point of view, phosphorus is an element that is associated with cell mass and organic constituents, nucleic acids contents and the level of cellular phosphorylation, and cells characterized by a severe structural damage show a decrease in intracellular concentrations of phosphorus [21]. Therefore, the high values found at P6 would confirm the vital status of these cells, whereas the decrease at P7 could be related to a possible structural damage of these cells.…”
Section: Discussionmentioning
confidence: 99%
“…We also expressed our data for Na, Cl, and K composition as a ratio of P concentration to obviate possible technical artifacts due to overpenetration of the electron probe in the sample, which may occur when EPXMA is used to analyze cells growing on solid supports (Roomans, 2002). According to criteria established by Abraham et al (1985), the use of elemental P ratios was possible because no significant differences in intracellular [P] between untreated control and STS‐treated cells.…”
Section: Discussionmentioning
confidence: 99%
“…In parallel with the decrease of the total K + and Na + content, a pronounced reduction in the Cl and P content was also found in apoptotic human monocytes [54]. As apoptosis and necrosis are both associated with a decrease in the K + /Na + ratio, the differences of element ratios may not allow the discrimination between those two types of cell death [52]. According to our observations, the sum of K + and Na + contents is lower in shrunken apoptotic cells vs normal cells.…”
Section: Discussionmentioning
confidence: 99%