Application of whole genome amplification for forensic analysis

Balogh, Márta; Børsting, Claus; Diz, Paula Sánchez; Thacker, C.R.; Syndercombe‐Court, Denise; Carracedo, Ángel; Morling, Niels; Schneider, P.M.

doi:10.1016/j.ics.2005.12.017

Cited by 8 publications

(8 citation statements)

References 6 publications

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“…The commercially available methods work optimally on 1-10 ng of DNA, although they have been successfully used on single or small numbers of cells in non-forensic areas [ 161 - 163 ], and generate sufficient DNA to analyse hundreds of genes. However, substantial amounts of amplification bias have been observed between homologous alleles and loci with low template amounts with WGA [ 164 , 165 ]. Despite this problem, promising results were seen in the use of WGA and specifically multiple displacement amplification (MDA), on forensically relevant trace DNA samples [ 165 - 167 ].…”

Section: Trace Dna Amplification and Detectionmentioning

confidence: 99%

“…However, substantial amounts of amplification bias have been observed between homologous alleles and loci with low template amounts with WGA [ 164 , 165 ]. Despite this problem, promising results were seen in the use of WGA and specifically multiple displacement amplification (MDA), on forensically relevant trace DNA samples [ 165 - 167 ]. STR profiling success was increased significantly, although peak height biases increased genotyping difficulty.…”

Section: Trace Dna Amplification and Detectionmentioning

confidence: 99%

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Forensic trace DNA: a review

2010

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DNA analysis is frequently used to acquire information from biological material to aid enquiries associated with criminal offences, disaster victim identification and missing persons investigations. As the relevance and value of DNA profiling to forensic investigations has increased, so too has the desire to generate this information from smaller amounts of DNA. Trace DNA samples may be defined as any sample which falls below recommended thresholds at any stage of the analysis, from sample detection through to profile interpretation, and can not be defined by a precise picogram amount. Here we review aspects associated with the collection, DNA extraction, amplification, profiling and interpretation of trace DNA samples. Contamination and transfer issues are also briefly discussed within the context of trace DNA analysis. Whilst several methodological changes have facilitated profiling from trace samples in recent years it is also clear that many opportunities exist for further improvements.

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Section: Trace Dna Amplification and Detectionmentioning

confidence: 99%

Section: Trace Dna Amplification and Detectionmentioning

confidence: 99%

Forensic trace DNA: a review

2010

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“…This amplified input quantity can fulfill the optimal requirement of different forensic STR typing kits and can give accurate and interpretable DNA profiling results. The use of WGA and multiple displacement amplification has been proven beneficial for generating STR profile from LCN and newer methodology arising in the field of WGA can prove useful for generating various STR's and SNP's DNA profiles (Balogh et al, 2006;Maciejewska, Jakubowska, & Pawłowski, 2013;Schneider et al, 2004) As PCR is used for making copies of a template DNA, therefore, by reducing the volume of PCR reaction mixture, the quality of the DNA template will not change. It has been found that it not only increases the sensitivity of the reaction by utilizing low quantity of sample, but also proves to be relatively inexpensive.…”

Section: Pcr Based Methods For Improved Lcn Techniquementioning

confidence: 99%

Generating DNA profile from low copy number DNA: Strategies and associated risks

Mateen¹,

Tariq

Hussain³

2020

Acta Sci. Biol. Sci.

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Many shreds of evidence found on the crime scenes contain a trace amount of DNA which results in insignificant profiling results for subsequent comparison. This can nullify the potential evidence material and hamper investigation process. Over the years, different strategies have been employed by various DNA testing laboratories to create interpretable DNA profiles generated from low template of DNA. This review highlights different strategies used by forensic laboratories worldwide for creating complete DNA profiles from low copy number template for comparison purposes along with its associated risks for forensic purposes

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“…WGA methods are used in a wide range of non-forensic genetic applications 28,29 and have been applied in a forensic context. 15,30,31 WGA can be achieved primarily by one of two approaches, being PCR-based amplification and multiple displacement amplification (MDA). 32 PCR-based amplification methods of WGA are conducted in the presence of random or degenerate primers and several methods have been developed for this approach, such as degenerate oligonucleotide primed-PCR (DOP-PCR), 33 primer extension preamplification (PEP), 34 improved primer extension preamplification (iPEP) 17,35 and modified-mproved primer extension preamplification (mIPEP).…”

Section: Introductionmentioning

confidence: 99%

“…16 It should be noted that a number of stochastic events, such as allelic bias and allelic dropout, were still produced using this method. 16,30,37…”

Section: Introductionmentioning

confidence: 99%

Evaluating the performance of whole genome amplification for use in low template DNA typing

et al. 2012

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We report on the performance of two whole genome amplification methods, GenomiPhi™ amplification and modified-improved primer extension preamplification (mIPEP), when analysing low template DNA samples. Template as low as 10 pg treated with mIPEP generated more than 1 ng of DNA that could be used in STR typing. Initial templates of 100-10 pg, when treated with mIPEP, generated an increase in alleles compared with control samples. Partial profiles using the AmpFℓSTR(®) Identifiler™ Kit were produced from this suboptimal DNA template, with 70% of the possible alleles (21.7 ± 2.1 in 32 alleles) recorded, using the mIPEP amplified products with an initial template of 100 pg. Allelic imbalance decreased with samples treated with whole genome amplification method (WGA) compared with those without this initial treatment. Further methods for improvement were also analysed including altering the condition of electrokinetic injection, and the successful DNA typing rate was increased to about 80%. This report illustrates the potential use and limitations of WGA for low template samples.

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Application of whole genome amplification for forensic analysis

Cited by 8 publications

References 6 publications

Forensic trace DNA: a review

Forensic trace DNA: a review

Generating DNA profile from low copy number DNA: Strategies and associated risks

Evaluating the performance of whole genome amplification for use in low template DNA typing

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