Application of water‐soluble polymers as modifiers in electrophoretic analysis of phenols

Abstract

A review of application of water-soluble cationic, anionic and nonionic polymers as pseudostationary phases in capillary electrophoresis (CE) and micellar electrokinetic capillary chromatography (MEKC) is presented. The effect of the structure of the polymers on the selectivity and efficiency of separation is discussed. A novel specially designed cationic polymer, 2,10-ionene, has been used for the separation of phenols. The polymer has hydrophilic and hydrophobic parts in its backbone. The polymer shows the best selectivity as a modifier in capillary zone electrophoresis (CZE)-mode, which allows the selective determination of both hydrophilic and hydrophobic phenols.

MEKC: An update focusing on practical aspects

“…MEKC has grown to such an extent over the period covered by this review (2002)(2003)(2004)(2005) that, for example, one of the emergent aspects examined in the previous review (viz the use of polymeric pseudostationary phases [16,17]), has been developed so extensively that it is now the subject of a dedicated review in this issue, so it has been omitted from this review. Microemulsion electrokinetic chromatography (MEEKC) [18,19], which can be regarded as an extension of MEKC, was not dealt with in the previous review; however, it has grown so fast and extensively in recent years that it warrants separate discussion and has thus also been excluded from this review.…”

Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications—Updated and completely revised edition

“…Tetrabutylammonium bromide (TBA) [138] Hexamethonium bromide [138] Diammonium hydrogen phosphate [138] PDADMA [138] Polyethyleneimine (PEI) [138] Camphor-sulfonate [139] Surfactants Sodium tetradecyl sulfate (STS) [139] Sodium hexadecyl sulfate (SHS) [139] Dodecyltrimethylammonium bromide (DTAB) [140] Tetradecyltrimethylammonium bromide (TTAB) [140] Polyoxyethylene sulfate (Brij-S) [141] Complexing reagents…”

Recent progress in the use of soluble ionic polymers as pseudostationary phases for electrokinetic chromatography

“…Figure 3 illustrates the separation of some substituted benzene and naphthalene compounds using 2,5-and 2,10-ionene. The chromatographic behavior of these ionenes and an aromatic analog was studied using alkyl-aryl ketones and phenols as analytes [30][31][32]. The length of the alkyl chains and the presence of aromatic functionality were shown to significantly alter the performance and selectivity of the polymers.…”