Application of VUV synchrotron radiation to proteomic and analytical mass spectrometry

Giuliani, Alexandre; Milosavljević, Aleksandar R.; Canon, Francis; Nahon, Laurent; Réfrégiers, Matthieu

doi:10.1088/1742-6596/425/12/122001

Cited by 2 publications

(2 citation statements)

References 13 publications

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“…In the frame of radiation damage, VUV photons offer a unique opportunity for understanding, at the molecular level, the fundamental physical processes following ionization. Moreover, the recent advances in intense tabletop VUV gas-discharge lamp sources have attracted increasing interest over the past years for the utilization of VUV light in mass spectrometry as a new tool for protein a e-mail: simon.doerner@desy.de (corresponding author) b e-mail: sadia.bari@desy.de (co-corresponding author) sequencing [3,4]. Today, while the popularity of this technique is growing, data on peptides and proteins are scarce (since carrying such experiments remains challenging) and fundamental photon-induced processes in play are yet to be understood.…”

Section: Introductionmentioning

confidence: 99%

The influence of the methionine residue on the dissociation mechanisms of photoionized methionine-enkephalin probed by VUV action spectroscopy

et al. 2021

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VUV action spectroscopy has recently gained interest for the study of peptides and proteins. However, numerous aspects of the fundamental processes involved in the photodissociation are yet to be understood. It can, for example, be expected that sulfur-containing amino-acid residues have a significant impact on the dissociation processes following photoionization because of their potential involvement in the transport of electron holes in proteins. In order to investigate the influence of the sulfur-containing methionine residue on the VUV photodissociation of a small peptide a VUV action spectroscopy study of gas-phase protonated methionine-enkephalin and leucine-enkephalin in the photon energy range of 6–35 eV was performed. The results show that upon non-ionizing photoexcitation, the fragmentation patterns of the two peptides are nearly identical, whereas significant differences were observed upon photoionization. The differences between the fragment yields and the identified specific dissociation channels for methionine-enkephalin could be explained by the high electron hole affinity of sulfur, which efficiently directs the radical to the methionine side chain. Additionally, for both peptides the presence of the intact photoionized precursor ions was confirmed by their isotopic patterns and the stability of these species could be evaluated. Graphic abstract

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Section: Introductionmentioning

confidence: 99%

The influence of the methionine residue on the dissociation mechanisms of photoionized methionine-enkephalin probed by VUV action spectroscopy

et al. 2021

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“…Examples are UV-visible photodissociation, [15][16][17] infrared and UV-visible [18][19][20] and infrared double resonance experiments 21,22 and blackbody infrared dissociation. 23,24 The main advantages of IRMPD spectroscopy over methods based on PID in other spectral regions [25][26][27][28][29][30][31] is that the IR spectral signatures allow the discrimination of functional groups, the analysis of conformational isomers and the identification of protonation/ coordination sites, especially when supported by theoretical calculations. 32,33 Consequently, IRMPD experiments have been used in an extensive number of systems and analytical applications, [34][35][36][37][38][39] from biomolecules, 40-42 drugs [43][44][45] and metabolites [46][47][48][49] to the evaluation of catalysis and reaction mechanisms, 19,[50][51][52][53][54][55][56] nanocalorimetry determinations 57,58 and in studies evaluating the electrospray ionization process 59 and aerosol composition.…”

Section: Introductionmentioning

confidence: 99%

Development of a photoinduced fragmentation ion trap for infrared multiple photon dissociation spectroscopy

Penna

Cervi

Rodrigues‐Oliveira

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale Methods for isomer discrimination by mass spectroscopy are of increasing interest. Here we describe the development of a three‐dimensional ion trap for infrared multiple photon dissociation (IRMPD) spectroscopy that enables the acquisition of the infrared spectrum of selected ions in the gas phase. This system is suitable for the study of a myriad of chemical systems, including isomer mixtures. Methods A modified three‐dimensional ion trap was coupled to a CO2 laser and an optical parametric oscillator/optical parametric amplifier (OPO/OPA) system operating in the range 2300 to 4000 cm−1. Density functional theory vibrational frequency calculations were carried out to support spectral assignments. Results Detailed descriptions of the interface between the laser and the mass spectrometer, the hardware to control the laser systems, the automated system for IRMPD spectrum acquisition and data management are presented. The optimization of the crystal position of the OPO/OPA system to maximize the spectroscopic response under low‐power laser radiation is also discussed. Conclusions OPO/OPA and CO2 laser‐assisted dissociation of gas‐phase ions was successfully achieved. The system was validated by acquiring the IRMPD spectra of model species and comparing with literature data. Two isomeric alkaloids of high economic importance were characterized to demonstrate the potential of this technique, which is now available as an open IRMPD spectroscopy facility in Brazil.

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Application of VUV synchrotron radiation to proteomic and analytical mass spectrometry

Cited by 2 publications

References 13 publications

The influence of the methionine residue on the dissociation mechanisms of photoionized methionine-enkephalin probed by VUV action spectroscopy

The influence of the methionine residue on the dissociation mechanisms of photoionized methionine-enkephalin probed by VUV action spectroscopy

Development of a photoinduced fragmentation ion trap for infrared multiple photon dissociation spectroscopy

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