Application of ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry (Orbitrap™) for the determination of beta-casein phenotypes in cow milk

Solah, Vicky; Busetti, Francesco; Smolenski, Grant; Cooney, Terry

doi:10.1016/j.foodchem.2019.125532

Cited by 28 publications

(11 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…de la β-caseína, la prolina en la variante A2, se sustituye por histidina en la variante A1 (111,112) . La variante A1 es más común en las razas lecheras populares en todo el mundo, como son la Holstein-Friesian, Red Danish, Ayrshire, Shorthorn y otras, mientras que la variante A2 es más frecuente en las razas Bos taurus: Jersey, Guernsey, Charolais, Limusina, razas Bos indicus, leche humana y otros (ovejas, cabras, burros, yaks, camellos, búfalos, etc).…”

Section: β-Caseína (Gen Csn2)unclassified

Estructura, propiedades y genética de las caseínas de la leche: una revisión

Doval¹,

Arteaga

2021

Ces. Med. Vet. Zootec.

View full text Add to dashboard Cite

La leche es un alimento esencial para los humanos y una de sus importancias radica en el contenido de proteínas lácteas. Las proteínas más frecuentes en este preciado líquido son las caseínas (αS1-caseína, αS2-caseína, β-caseína y κ-caseína), las cuales son fuente de aminoácidos para la dieta de los mamíferos en sus primeros días de vida. En la leche, las caseínas, están formadas por agregados moleculares de proteínas de tamaños variables denominados micelas. El objetivo de esta revisión es presentar un panorama general de la estructura, propiedades y genética de las caseínas lácteas y su relación con la salud humana. A partir de esta revisión, se pudo establecer, que las αs1 y αs2 caseínas se encuentran en conjunto con la β-caseína, formando el núcleo micelar, interactuando con los iones de calcio, para formar y mantener la micela estable. Animales caracterizados genéticamente con algunas variantes de estas proteínas, se asocian con un rendimiento en el volumen de leche. La κ-caseína, por su parte, está asociada con un aumento en el rendimiento y calidad de los quesos, de ahí su importancia económica, mientras que las formas más comunes de β-caseína en razas de ganado lechero son A1 y A2. La β-caseína A2 no presenta efectos negativos a la salud humana, por el contrario, ha sido asociada con propiedades reductoras de colesterol y triacilglicéridos. Sin embargo, la variante A1 de la β-caseína produce un péptido bioactivo denominado β-casomorfina-7 (BCM-7), que puede desempeñar un papel etiológico poco claro en el desarrollo de algunas enfermedades en humanos, tales como: enfermedad isquémica del corazón, diabetes mellitus tipo 1, síndrome de muerte súbita infantil (SIDS), desórdenes neurológicos, como autismo y esquizofrenia.

show abstract

Section: β-Caseína (Gen Csn2)unclassified

Estructura, propiedades y genética de las caseínas de la leche: una revisión

Doval¹,

Arteaga

2021

Ces. Med. Vet. Zootec.

View full text Add to dashboard Cite

show abstract

“…At present, most of the international research on BCM-7 focuses on phenotypic identification (Nguyen, Solah et al 2020), digestion and degradation (Asledottir, Picariello et al 2019) and a part of the latest research confirms that BCM-7 has improved lactose intolerance(Uscanga-Domínguez, Orozco-García et al 2019). Previous experiments proved that BCM-7 could relieve the damage of small intestinal mucosa caused by free radicals produced by hyperglycemia oxidative stress (Han, Zhang et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Effect of Beta-casomorphin-7 on Renal Function and Immunity in Aging Mice

Yin¹,

Chen²,

Su³

et al. 2021

IJAR

View full text Add to dashboard Cite

Background: Most studies have shown that Beta-Casomorphin-7 (BCM-7) has many physiological functions, this study aims to investigate the effects of BCM-7 on renal function and immunity in naturally aging mice and to reveal the effects of BCM-7 on health and provide more theoretical reference for inflammatory aging.Methods: All mice were weighed weekly and the organ indexes were calculated, Paraffin sections were prepared for kidney histopathological examination with conventional Haematoxylin and Eosin (HE) staining, renal function indexes and cytokines were main measured by ELISA and biochemical colorimetry.Result: The weight of each group increased during gastric perfusion and the higher the intervention concentration, the lower the weight gain rate. The renal index was not affected by BCM-7, but the spleen index was significantly decreased by medium-dose BCM-7. There were inflammatory cell infiltration and other renal lesions in the aging control group and intervention group. A medium dose of BCM-7 can significantly reduce Cystatin C (Cys-C) content in aging mice, the content of Adrenocorticotropic hormone (ACTH) in aged mice decreased with the increase of BCM-7 intervention dose. BCM-7 and aging caused cytokine imbalance in mice.

show abstract

“…Many works have been done about the analysis of β-casein natural variants in cow milk. The main detection methods include gel electrophoresis [11], CE [17], RPLC (HPLC) [18], LCMS (HPLC-MS) [19][20][21]. Methods, such as gel electrophoresis, CE, and HPLC effectively separate some variants, such as A1, A2, B, C, etc., but it is challenging to identify the separated components.…”

Section: Introductionmentioning

confidence: 99%

“…Givens et al [19] used HPLC-MS to detect the intact proteins of four variants A1, A2, B, and C in pasteurized milk sold in the UK, and used peak area normalization to analyze the relative differences of each variant. Nguyen et al [20] determined the β-casein phenotypes in cow milk from Holstein Friesian and cross-bred Holstein Friesian dairy cattle in Phu Dong and three variants, included A1, A2, and I, were identified. Fuerer et al [12] used UHPLC electrostatic field orbitrap high-resolution MS to detect and analyze milk protein fingerprints in infant powder, and quantitatively detect the content of A1, A2 β-casein in skimmed milk powder.…”

Section: Introductionmentioning

confidence: 99%

Analysis of A1‐type and A2‐type β‐casein in Maiwa Yak and Pien‐niu milk by HPLC‐high‐resolution MS and tandem MS

Chen

Ren

Wang

et al. 2021

J of Separation Science

View full text Add to dashboard Cite

In this study, a peptide-based method employing ultra high performance liquid chromatography electrostatic field orbitrap high-resolution mass spectrometry and triple quadrupole mass spectrometry was developed for quantification of A1type and A2-type β-casein in milk from Yak, cows, and their offspring of crosses, Pien-niu. The specific peptides of A1-type and A2-type β-casein were screened and confirmed by protein software after analysis of high-resolution mass spectrometry. The multiple reaction monitoring method was established based on the qualitative results, and isotope-label peptides were used as internal standards.The linear correlation coefficients of this method were >0.99. The relative standard deviations of repeatability test were 0.2-3.6%. The recovery rate ranged from 93.3 to 114.4% with relative standard deviations <6% at three different spiking levels. The method was applied to analyze 45 milk samples from different species.The results showed that β-casein in Yak and Pien-niu milk was about 30% higher than that in cow milk. Furthermore, the β-casein in the Yak milk only contains A2-type β-casein. A1-type and A2-type β-casein coexist in most samples of Pienniu and cow milk, a few samples contain only one type of β-casein. These results provide further understanding in nutritional value of milk from Yak and Pienniu.

show abstract

Application of ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry (Orbitrap™) for the determination of beta-casein phenotypes in cow milk

Cited by 28 publications

References 17 publications

Estructura, propiedades y genética de las caseínas de la leche: una revisión

Estructura, propiedades y genética de las caseínas de la leche: una revisión

Effect of Beta-casomorphin-7 on Renal Function and Immunity in Aging Mice

Analysis of A1‐type and A2‐type β‐casein in Maiwa Yak and Pien‐niu milk by HPLC‐high‐resolution MS and tandem MS

Contact Info

Product

Resources

About