The study was conducted to validate in vitro prediction of standardised ileal digestibilities (SID) of crude protein (CP) and amino acids (AA) in grain legumes for growing pigs using six different cultivars of faba beans (Vicia faba), six different cultivars of field peas (Pisum sativum), and five different cultivars of lupins (Lupinus spp.). The SID for CP and AA were predicted from in vitro analysis by means of a two-step enzymatic method using pepsin and pancreatin incubations. In vitro predicted SID values of CP and AA were generally higher than the corresponding SID values measured in vivo. There were strong linear relationships (r 2 5 0.73 for Lys to r 2 5 0.91 for Cys and Trp) between in vivo and in vitro predicted SID values in the assay feed ingredients if grain legume species (i.e. faba beans, field peas and lupins) was included as a covariate in multiple linear regression analysis. However, to rapidly and accurately predict SID of CP and AA in individual batches of various feed ingredients, further studies are warranted.Keywords: standardised ileal digestibility, in vitro, grain legumes, amino acids, pigs
ImplicationsClose linear relationships between in vivo standardised ileal digestibilities (SID) and in vitro predicted SID values for crude protein (CP) and amino acids (AA) in faba beans, field peas and lupins were obtained. However, probably due to the presence of anti-nutritional factors, differences between in vitro predicted SID and in vivo SID values varied between and within these grain legume species. Thus, further validation of the in vitro assay based on the results of in vivo SID studies is required before being used for the prediction of SID of CP and AA in feed ingredients and diets for pigs.