Application of transposon insertion site sequencing method in the exploration of gene function in microalgae

Abstract

Microalgae are a large group of organisms that can produce various useful substances through photosynthesis. Microalgae need to be genetically modified at the molecular level to become “Chassis Cells” for food, medicine, energy, and environmental protection and, consequently, obtain benefits from microalgae resources. Insertional mutagenesis of microalgae using transposons is a practical possibility for understanding the function of microalgae genes. Theoretical and technical support is provided in this manuscript for applying transposons to microalgae gene function by summarizing the sequencing method of transposon insertion sites.

Shedding light on bacteria–host interactions with the aid of TnSeq approaches

“…2B ). TnSeq-related methods have even been successfully applied in other organisms, such as yeast ( 56 ), microalgae ( 57 ), archaea ( 58 ), and amoeba ( 44 ). For simplicity purposes, in this review we will focus only on the research conducted in bacterial species.…”