Application of the water-insoluble, temperature-responsive block polymer poly(butyl methacrylate-block-N-isopropylacrylamide) for pluripotent stem cell culture and cell-selective detachment

Kuno, Goshi; Imaizumi, Yu; Matsumoto, Akikazu

doi:10.1016/j.jbiosc.2022.02.005

Cited by 4 publications

(1 citation statement)

References 29 publications

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“…The colloids were randomly dispersed on the substrate. In our previous study, we reported the selective detachment of iPSCs from cocultured iPSC-derived differentiated cells through the formation of nanostructures using a thermoresponsive polymer [34]. Because iPSCs detached easily from colloidal arrays, coculture of iPSCs and iPSC-derived mesoderm cells was performed to confirm the selective detachment.…”

Section: Effects Of Colloid Density On Ipsc Proliferation and Adhesionmentioning

confidence: 99%

Easy Cell Detachment and Spheroid Formation of Induced Pluripotent Stem Cells Using Two-Dimensional Colloidal Arrays

Kuno

Matsumoto

2023

Macromol

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Induced pluripotent stem cells (iPSCs) may develop into any form of cell and are being intensively investigated. The influence on iPSCs of nanostructures generated using two-dimensional colloidal arrays was examined in this study. Colloidal arrays were formed using the following procedure. First, core–shell colloids were adsorbed onto a glass substrate using a layer-by-layer method. Second, the colloids were immobilized via thermal fusion. Third, the surface of the colloids was modified by plasma treatment. By adjusting the number density of colloids, cultured iPSCs were easily detached from the substrate without manual cell scraping. In addition to planar culture, cell aggregation of iPSCs attached to the substrate was achieved by combining hydrophilic surface patterning on the colloidal array. Multilayered cell aggregates with approximately four layers were able be cultured. These findings imply that colloidal arrays might be an effective tool for controlling the strength of cell adhesion.

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Section: Effects Of Colloid Density On Ipsc Proliferation and Adhesionmentioning

confidence: 99%

Easy Cell Detachment and Spheroid Formation of Induced Pluripotent Stem Cells Using Two-Dimensional Colloidal Arrays

Kuno

Matsumoto

2023

Macromol

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Temperature-modulated interactions between thermoresponsive strong cationic copolymer-brush-grafted silica beads and biomolecules

Nagase,

Suzuki,

Kanazawa

2024

Heliyon

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Thermo-responsible PNIPAM-grafted polystyrene microspheres for mesenchymal stem cells culture and detachment

Zhao,

Cao,

Zhang

et al. 2024

Biomed. Mater.

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The preparation of cells is a critical step in cell therapy. To ensure the effectiveness of cells used for clinical treatments, it is essential to harvest adherent cells from the culture media in a way that preserves their high viability and full functionality. In this study, we developed temperature-responsive PNIPAM-grafted polystyrene microspheres using reversible addition-fragmentation chain transfer polymerization. These microspheres allow for the non-destructive harvesting of cultured cells through temperature changes. The composition and physicochemical properties of the PNIPAM-grafted polystyrene microspheres were confirmed using infrared spectroscopy, elemental analysis, dynamic light scattering, and thermogravimetric analysis. In vitro experiments demonstrated that these microspheres exhibit excellent biocompatibility, supporting the adhesion and proliferation of various cells. Moreover, the microspheres showed good temperature responsiveness in thermosensitive detachment experiments with GFP-HepG2 cells and umbilical cord mesenchymal stem cells (UC-MSCs). Additionally, through orthogonal experiments, we identified a cell detachment aid mixture that significantly improved the dispersibility of cells detached from the microspheres, enhancing the efficiency of thermosensitive cell detachment by approximately 40%. The harvested UC-MSCs retained their capacity for re-proliferation and trilineage differentiation. Consequently, the temperature-responsive microspheres developed in this study, combined with the cell detachment aid mixtures, hold great potential for large-scale culture and harvesting of therapeutic cells in clinical applications.

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Application of the water-insoluble, temperature-responsive block polymer poly(butyl methacrylate-block-N-isopropylacrylamide) for pluripotent stem cell culture and cell-selective detachment

Cited by 4 publications

References 29 publications

Easy Cell Detachment and Spheroid Formation of Induced Pluripotent Stem Cells Using Two-Dimensional Colloidal Arrays

Easy Cell Detachment and Spheroid Formation of Induced Pluripotent Stem Cells Using Two-Dimensional Colloidal Arrays

Temperature-modulated interactions between thermoresponsive strong cationic copolymer-brush-grafted silica beads and biomolecules

Thermo-responsible PNIPAM-grafted polystyrene microspheres for mesenchymal stem cells culture and detachment

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