Application of the UV–vis spectrophotometry method for the determination of glutamate in the cerebrospinal fluid of rats

Ferreira, Cirley Pinheiro; Antunes, Flavia Tasmin Techera; Rebelo, Isadora Nunes; Silva, Claudio Antônio da; Vilanova, Fernanda Nunes; Corrêa, Dione Silva; Souza, Alessandra Hübner de

doi:10.1016/j.jpba.2020.113290

Cited by 10 publications

(2 citation statements)

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“…High performance liquid chromatography (HPLC) is a physical-chemical separation technique that, coupled with ultraviolet-visible (UV-Vis) detection, has become one of the most used methods for quantitative analysis of various bioactives, due to its high sensitivity, selectivity and versatility. However, HPLC requires preliminary sample preparation, such as solvent extraction and separation of interfering metal ions, which makes this analysis more time consuming, in addition to demanding a high cost associated with the use of an expensive equipment, along with specific analyst training requirements (Albuquerque et al, 2020;Ferreira et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…These limitations can be overcome by employing simpler techniques, such as UV-Vis absorption spectrophotometry. Spectrophotometric determinations are simple and accessible, accurate, reproducible and inexpensive, as they do not require the use of sophisticated analytical instruments, in addition to providing fast, reliable and easy-to-interpret results (Ferreira et al, 2020;Nalini & Kumar, 2020). However, since this technique may lack selectivity, this parameter should be controlled, especially when the analyte is in the presence of a complex matrix, such as the case of vegetable oils.…”

Section: Introductionmentioning

confidence: 99%

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Low-cost method for quantification of salicylic acid loaded in lipid nanoparticles containing copaiba oil

Rocha

Viana

Silveira

et al. 2022

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The demand for analytical methods for drug quantification is an obstacle in the research and development of new products, due to the need to use simpler techniques for the identification and quantification of the actives. Although it is a very sensitive and selective technique for these determinations, high performance liquid chromatography (HPLC) requires expensive instrumentation and well-trained analysts, while spectrophotometry is fast, low-cost and hardly requires sample pre-treatment. Thus, this study aimed to develop a quantification method by UV-visible spectrophotometry for salicylic acid (SA) encapsulated in nanostructured lipid carriers (NLC) containing copaiba oil. The method was validated according to national and international criteria (ANVISA, ICH and FDA). The diluent was a mixture of absolute ethanol and phosphoric acid (99:1) and detection was performed at 310 nm. The interference of the matrix in relation to NLC-SA was minimal (0.37%), demonstrating the selectivity. Linearity was obtained in the range of 5.0 30.0 μg/mL (r = 0.99948) and the method proved to be accurate, with high recovery of SA in the presence of the other components (over 96% recovery), with precision at two levels: intra- and inter-days (DPR < 5%). Detection and quantification limits were 0.51 e 1.70 μg/mL, respectively. Finally, according to the Youden test criteria, the method was robust in the face of the proposed variations, obtaining effect values lower than the critical value (0.07). Therefore, the developed method is accessible, effective and valid for the quantification of SA in complex matrix lipid nanoparticles.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%