Application of the Spectrophotometric Method for Determination of the Cell Numbers of Microalgae in the Genus Tetraselmis (Chlorophyta): Calibration Curves and Equations for Calculation

Маркина, Ж. В.; Maslennikov, S. I.; Botsun, Liudmnila

doi:10.1134/s1063074022060098

Cited by 2 publications

(1 citation statement)

References 24 publications

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“…Although these wavelengths can be used by means of standard curves as predictors of chlorophyll content, and the same is true for other wavelengths assigned to other pigment peaks [62][63][64], they are not the best predictors of biomass as found in the present study. Instead, the wavelength of 750 nm can reliably predict with sufficient accuracy algal density [54,56,[65][66][67][68][69][70] because this wavelength avoids the light absorption by photosynthetic pigments which might affect its absorbance value created by turbidity alone [48,56,71]. In both species of microalgae studied, its correlation with the algal density gave very high correlation coefficients and, as this was found also in my previous studies [45][46][47]55,56], leaves me with no doubt for its usefulness in the daily monitoring routine of an algal culture.…”

Section: Discussionmentioning

confidence: 66%

Quantity and Quality of Light on Growth and Pigment Content of Dunaliella sp. and Anabaena sp. Cultures and the Use of Their Absorption Spectra as a Proxy Method for Assessment

Hotos

2023

JMSE

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(1) Background: As microalgae cultures are affected by the quantity and quality of light, I explored this for two species. Additionally, I introduced a novel easy and economical way for the growers to easily and economically ascertain continuously with satisfactory accuracy the quantitative and qualitative status of their culture using absorption spectra. (2) Methods: The locally isolated chlorophyte Dunaliella sp. and the cyanobacterium Anabaena sp. were cultured in small volumes with two intensities of white light (2000 and 8000 lux) and with green, blue and red light, and the increase in their biomass and pigments was studied. Pigment analyses, continuous recordings of absorption spectra and calibration curves were used. (3) Results: The intensity of 8000 lux of white light yielded the highest increase in biomass, chlorophylls and carotenoids in Dunaliella sp., and the same was found for green and blue light, while 2000 lux and green light caused the greatest increase in biomass and phycocyanin in Anabaena sp. From the absorption spectra, the evolution of the pigment content can be estimated, and both pigments and biomass are correlated very strongly with those extracted from the spectra absorption of 750 nm. (4) Conclusions: The use of absorption spectra as an easy, fast and economical method can be a useful tool for a good approximation of the state of the microalgae culture. This is clearly shown when the spectra of the cultures under different light intensities and colors are compared having a catalytic effect on the level of the pigments leading to the increase in carotenoids and phycocyanin of the green light.

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Section: Discussionmentioning

confidence: 66%

Quantity and Quality of Light on Growth and Pigment Content of Dunaliella sp. and Anabaena sp. Cultures and the Use of Their Absorption Spectra as a Proxy Method for Assessment

Hotos

2023

JMSE

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Quantity and Quality of Light on Growth and Pigment Content of <em>Dunaliella</em> sp. and <em>Anabaena</em> sp. Cultures and the Use of Their Absorption Spectra as a Proxy Method for Assessment

Hotos¹

2023

Preprint

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(1) Background: Microalgae cultures are greatly facilitated if growers can easily and economically ascertain the quantitative and qualitative status of the culture continuously with satisfactory ac-curacy. (2) Methods: The locally isolated microalgae Dunaliella sp. and Anabaena sp. were cultured in small volumes with 2 intensities of white light (2000 and 8000 lux) and with green, blue and red light and the increase of their biomass and pigments was studied. Pigment analyses, continuous recordings of absorption spectra and calibration curves were performed. (3) Results: The intensity of 8000 lux white light yielded the highest increase in biomass, chlorophylls and carotenoids in Dunaliella sp. while 2000 lux and green light caused the greatest increase in biomass and phy-cocyanin in Anabaena sp. From the examination of the absorption spectra, the evolution of the pigment content can be estimated and both pigments and biomass are correlated very satisfactorily with the wavelength of 750 nm. (4) Conclusions: The use of absorption spectra as an easy, fast and economical method can be a useful tool for a good approximation of the state of the microalgae culture. This is clearly shown when the spectra of the culture under different lighting colors are compared which have a catalytic effect on the level of the pigments leading to the increase in carotenoids and phycocyanin of the green light.

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Application of the Spectrophotometric Method for Determination of the Cell Numbers of Microalgae in the Genus Tetraselmis (Chlorophyta): Calibration Curves and Equations for Calculation

Cited by 2 publications

References 24 publications

Quantity and Quality of Light on Growth and Pigment Content of Dunaliella sp. and Anabaena sp. Cultures and the Use of Their Absorption Spectra as a Proxy Method for Assessment

Quantity and Quality of Light on Growth and Pigment Content of Dunaliella sp. and Anabaena sp. Cultures and the Use of Their Absorption Spectra as a Proxy Method for Assessment

Quantity and Quality of Light on Growth and Pigment Content of <em>Dunaliella</em> sp. and <em>Anabaena</em> sp. Cultures and the Use of Their Absorption Spectra as a Proxy Method for Assessment

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