Application of the skin and bone integrated pylon with titanium oxide nanotubes and seeded with dermal fibroblasts

Abstract

Study Design The feasibility and safety of in bone implantation of the skin and bone integrated pylons (SBIP) with nanotubes was investigated in vitro and in vivo in the animal model. Background Direct Direct skeletal attachment of limb prostheses is associated with high rate of transcutaneous infection and loosening of the fixture in the medullary canal prompting for careful assessment of various means for enhancing the skin-device and bone-device interface. The SBIP system constitutes a technological platform for different modifications being evaluated previously. Objectives The current study assessed the combination of nano treatment SBIP with its pre seeding with dermal fibroblasts. We hypothesized that this combination will enhance cell interaction with SBIP compared to nano treatment and the fibroblast seeding when done separately. Methods TiO2 nanotubes were fabricated on the SBIP, and the fibroblasts taken from rabbit's skin were cultured on the pylons before implantation. Results The in vitro experiments demonstrated higher cellular density in the samples with a nanotubular surface than in the non modified pylons used as control. There were no postoperative complications in any of the animals during the 6 month observation period. Subsequent SEM of the pylon extracted from the rabbit's femur showed the stable contact between the pylon and soft tissues in comparison to control samples where the patchy fibrovascular ingrowth was detected. Conclusions The promising results prompt further investigation of the integrative properties of the nanotextured SBIP system seeded with dermal fibroblasts and its optimization for clinical application.

Cost-effectiveness of bone-anchored prostheses using osseointegrated fixation

“…Clearly, there will be a need to further extend this study to accommodate future developments in BAP including, but not limited to, growing number of consumers, broadening of case mix, changes to surgical procedures and constant developments of prosthetic components. 51,53,54 Effects of these changes in the economic evaluation of BAP over aids for non-prosthetic user (e.g. crutches, wheelchair) and other fitting options for prosthetic users (e.g.…”

Evaluation of the temporary effect of physical vapor deposition silver coating on resistance to infection in transdermal skin and bone integrated pylon with deep porosity

“…The patented specifications of the implant include porosity, pore size, particle size and volume fraction of the material composition for sintering and the specific silver coating . High biointegrative and mechanical properties of the SBIP implants in various animal species including rats, mice, rabbits, and cats were demonstrated . The safety and sustainability of the skin‐device and bone‐device interface is provided for by the SBIP's composite porous structure, which promotes deep ingrowth of the hosting skin into the implanted pylon.…”

Improved osseointegration properties of hierarchical microtopographic/nanotopographic coatings fabricated on titanium implants

“…5 Previously, it was demonstrated that the nanostructured surface of the implants significantly improves the biocompatibility. 6 Nanostructured organization of the implants (eg, nanotubes, nanofibers, nanowires, and nanorods) promotes the integration of the implants with the surrounding bone tissues. [7][8][9][10][11][12] Thus, in the study by Seidling et al, the tested Ti implants with the nanostructured hyperhydrophilic chromosulfuric acid surface demonstrated 17-fold increase in the bone-to-implant contact and de novo bone formation in a gap healing model using Göttingen minipigs.…”