Application of the pathogen Trojan horse approach in maize (Zea mays)

Linde, Karina van der; Egger, Rachel L.; Timofejeva, Ljudmilla; Walbot, Virginia

doi:10.1080/15592324.2018.1547575

Cited by 4 publications

(2 citation statements)

References 24 publications

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“…To test the responses of a biotrophic pathogen to the maize phytocytokines we made use of the “trojan horse” approach in which the fungus Ustilago maydis is deployed to deliver plant peptides into the leaf apoplast in-vivo (van der Linde et al, 2018; Ziemann et al, 2018). U. maydis infects primordia of all aerial organs of maize and virulence can be evaluated by the induction of chlorosis, anthocyanin and tumor formation (Schilling et al, 2014; Skibbe et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

Maize phytocytokines and microbial-patterns trigger antagonistic features in co-incidence with wounding and fungal pathogens

Koenig

Moser

Leusner

et al. 2022

Preprint

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Phytocytokines are signalling peptides that activate immune responses and alarm bystander cells of danger. The pathways of phytocytokine perception and activation are thought to be shared between exogenous danger signals such as microbe-associated molecular patterns (MAMPs) and endogenous, passively released, damage-associated molecular patterns (DAMPs). However, downstream responses triggered by danger molecules and their effect in plant survival is still largely unknown. Here, we have identified three biologically active maize orthologues of phytocytokines previously described in other plants. The maize phytocytokines show common features with MAMPs, including the induction of immune related genes and activation of papain-like cysteine proteases. In contrast to MAMPs, the phytocytokines do not promote cell death in the presence of wounding. In infection assays with fungal pathogens of two different life styles we found that phytocytokines affect the development of disease symptoms, likely due to the activation of phytohormonal pathways. Collectively, our results show that phytocytokines and MAMPs trigger unique and antagonistic features of immunity. We propose a model in which phytocytokines activate immune responses partially similar to MAMPs but in contrast to microbial signals, they act as danger and survival molecules to the surrounding cells. Future studies will focus on the components determining the divergence of signalling outputs upon phytocytokine activation.

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Section: Resultsmentioning

confidence: 99%

Maize phytocytokines and microbial-patterns trigger antagonistic features in co-incidence with wounding and fungal pathogens

Koenig

Moser

Leusner

et al. 2022

Preprint

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“…Furthermore, Brassinosteroids have also been described in connection with ROS regulation in anther development as detailed below (Yan et al 2020). In contrast, during early anther development, signaling is not mainly based on phytohormones (Walbot and Skibbe 2010;; van der Linde and Walbot 2019) but rather on environmental clues and other small mobile signals which orchestrate cell-cell communication (Kelliher and Walbot 2012;Zhang and Yang 2014;van der Linde et al 2018b).…”

Section: Mobile Elements In Anther Developmentmentioning

confidence: 99%

Oxygen, secreted proteins and small RNAs: mobile elements that govern anther development

Dukowic-Schulze

Linde

2021

Plant Reprod

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Correct anther development is essential for male fertility and subsequently agricultural yield. Defects in anther development range from the early stage of stamen formation until the late stage of tapetum degeneration. In particular, the specification of the four distinct somatic layers and the inner sporogenous cells need perfect orchestration relying on precise cell–cell communication. Up to now, several signals, which coordinate the anther´s developmental program, have been identified. Among the known signals are phytohormones, environmental conditions sensed via glutaredoxins, several receptor-like kinases triggered by ligands like MAC1, and small RNAs such as miRNAs and the monocot-prevalent reproductive phasiRNAs. Rather than giving a full review on anther development, here we discuss anther development with an emphasis on mobile elements like ROS/oxygen, secreted proteins and small RNAs (only briefly touching on phytohormones), how they might act and interact, and what the future of this research area might reveal.

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A Trojan Horse Approach Using Ustilago maydis to Study Apoplastic Maize (Zea mays) Peptides In Situ

Kutzner,

van der Linde

2023

Methods in Molecular Biology

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Application of the pathogen Trojan horse approach in maize (Zea mays)

Cited by 4 publications

References 24 publications

Maize phytocytokines and microbial-patterns trigger antagonistic features in co-incidence with wounding and fungal pathogens

Maize phytocytokines and microbial-patterns trigger antagonistic features in co-incidence with wounding and fungal pathogens

Oxygen, secreted proteins and small RNAs: mobile elements that govern anther development

A Trojan Horse Approach Using Ustilago maydis to Study Apoplastic Maize (Zea mays) Peptides In Situ

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