Application of the micronucleus test to exfoliated epithelial cells from the oral cavity of beedi smokers, a high-risk group for oral cancer

Suhas, S; Ganapathy, KS; Gayatridevi,; Ramesh, C.

doi:10.1016/j.mrgentox.2004.03.001

Cited by 41 publications

(27 citation statements)

References 30 publications

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“…These include scraping with a wood spatula (31,34,48,51,52,60,62,91,92), wood tongue depressor (12,32,43,45,56), cotton swab (42), short-bristle cytobrush (28,29,33,40,44,57,95,96), and toothbrush (95,96).…”

Section: Resultsmentioning

confidence: 99%

Smoking and Smokeless Tobacco-Associated Human Buccal Cell Mutations and Their Association with Oral Cancer—A Review

Proia

Paszkiewicz

Nasca³

et al. 2006

Cancer Epidemiology, Biomarkers &Amp; Prevention

142

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Reported herein are the results of a structured literature review that was undertaken to (a) determine if human buccal (mouth) cell changes are associated with smoking and smokeless (''chewing'') tobacco, (b) tabulate different buccal cell alterations that have been reported, (c) delineate buccal cell assays that have been used successfully, (d) determine whether buccal cell changes correlate with oral cancer as defined in clinicopathologic investigations, and (e) assess the feasibility of developing a high-throughput buccal cell assay for screening smokers for the early detection of oral cancer. The results of the studies reported herein have established that diverse buccal cell changes are associated with smoking and smokeless tobacco. This review documents also that buccal cells have been collected in a noninvasive manner, and repetitively for serial studies, from different sites of the mouth (e.g., cheek, gum, and tongue) and from normal tissue, preneoplastic lesions (leukoplakia), and malignant tumors. Tobacco-associated genetic mutations and nongenetic changes have been reported; a partial listing includes (a) micronuclei, (b) bacterial adherence, (c) genetic mutations, (d) DNA polymorphisms, (d) carcinogen-DNA adducts, and (e) chromosomal abnormalities. Clinical studies have correlated buccal cell changes with malignant tumors, and some oral oncologists have reported that the buccal cell changes are practical biomarkers. Summarily, the literature has established that buccal cells are useful not only for characterizing the molecular mechanisms underlying tobacco-associated oral cancers but also as exfoliative cells that express diverse changes that offer promise as candidate biomarkers for the early detection of oral cancer. (Cancer Epidemiol Biomarkers Prev 2006;15(6):1061 -77)

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Section: Resultsmentioning

confidence: 99%

Smoking and Smokeless Tobacco-Associated Human Buccal Cell Mutations and Their Association with Oral Cancer—A Review

Proia

Paszkiewicz

Nasca³

et al. 2006

Cancer Epidemiology, Biomarkers &Amp; Prevention

142

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“…The MN frequency detected in the palatal and buccal mucosa epithelial cells of individuals who smoke cigarette was significantly higher than that of the control group subjects, while the increase in MN frequency in the lingual epithelial cells was insignificant [27]. …”

Section: Discussionmentioning

confidence: 99%

Determination of Genotoxic Effects of Hookah Smoking by Micronucleus and Chromosome Aberration Methods

Eker

Koyuncu²,

Şahin

et al. 2016

Med Sci Monit

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BackgroundUse of a hookah (a type of water pipe) is a traditional way of smoking tobacco, particularly in the Middle East. In Turkey, its popularity has been growing in recent years, especially among young people. It is known that cigarette smoking has genotoxic effects and causes mutations, but no comprehensive study has been done on the genotoxic effects of hookah usage, particularly in Turkey.Material/MethodsWe collected peripheral blood/buccal smear samples from 30 subjects who did not smoke cigarettes but who regularly smoke a hookah an average of 2 times per week, and from 30 control subjects who had never smoked cigarettes or a hookah. Chromosome analyses were performed on the samples obtained from peripheral blood of each individual, 25 metaphase plaques were counted for each, and chromosome/chromatid breakage/gap parameters were evaluated. Micronucleus analysis was done on buccal smear samples and micronucleus/binucleus parameters were investigated by counting 2000 cells of each individual.ResultsChromosome breakage ratios were found to be 0.64±0.86 and 0.46±0.71 in the study and control groups, respectively, while chromatid breakage ratios were 0.53±0.83 and 0.53±0.71; fragment ratios were 0.82±1.24 and 0.21±0.49 (p<0.05); and gap ratios were 0.57±0.83 and 0.18±0.53 (p<0.05), respectively. Micronucleus ratio was 6.03±2.06 and 4.43±2.27 (p<0.05) in the study and control groups, respectively, and binucleus ratios were 8.53±3.23 and 12.15±5.18, respectively (p<0.05).ConclusionsResults of our study reveal significant statistical differences between the individuals who smoked hookah and those who did not in terms of fragment, gap, micronucleus, and binucleus parameters, suggesting that smoking a hookah may cause genotoxic effects.

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“…In the study by Stich and Rosin [8], the number of micronuclei of heavy smokers was similar to that of non-smokers. Suhas et al [9] showed an increased frequency of micronuclei in smokers of beedi, in which concentrations of nicotine, tar, and other toxic agents in smoke from burnt tobacco are higher than in cigarette smoke. The data presented by Wu et al [10] revealed that cigarette smoking did not increase the number of micronuclei in smokers when compared with controls.…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic Biomonitoring in Buccal Mucosa Cells from Young Smokers

et al. 2015

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Objective: Nowadays, much attention has been focused on the search for new non-invasive methodologies able to predict malignant transformation of oral mucosa cells. The aim of the present study was to comparatively evaluate DNA damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) in exfoliated oral mucosa cells from smokers and non-smokers in buccal mucosa cells. Study Design: A total of 24 young, healthy smokers and 14 non-smokers were included in this setting. Individuals had epithelial cells from the cheek mechanically exfoliated, placed in fixative and dropped in clean slides which were checked for the above nuclear phenotypes. Results: Smokers presented more (p < 0.05) micronucleated oral mucosa cells than non-smokers. Tobacco smoke was not able to increase other nuclear alterations closely related to cytotoxicity such as karyorrhexis, pyknosis and karyolysis. Conclusion: In summary, these data indicate that the cigarette is able to induce micronuclei in oral mucosa cells, so the micronucleus test is a suitable method for predicting oral cancer risk.

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Application of the micronucleus test to exfoliated epithelial cells from the oral cavity of beedi smokers, a high-risk group for oral cancer

Cited by 41 publications

References 30 publications

Smoking and Smokeless Tobacco-Associated Human Buccal Cell Mutations and Their Association with Oral Cancer—A Review

Smoking and Smokeless Tobacco-Associated Human Buccal Cell Mutations and Their Association with Oral Cancer—A Review

Determination of Genotoxic Effects of Hookah Smoking by Micronucleus and Chromosome Aberration Methods

Cytogenetic Biomonitoring in Buccal Mucosa Cells from Young Smokers

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