Application of the CellDetect® staining technique in diagnosis of human cervical cancer

He, Song; Wang, Guilan; Zhu, Yan; Mei-hua, WU; Zhi-guo, JI; Seng, Juan; Jing, Yi; Zhou, Jiaming; Chen, Li

doi:10.1016/j.ygyno.2013.12.016

Cited by 13 publications

(7 citation statements)

References 7 publications

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“…The main advantage of Pap staining is that it clearly shows the fine structure of the nucleus. The cytoplasm is bright and transparent, reflecting the change in the differentiation of the cytoplasm (44,45). Diff-Quick staining is a fast staining method modified on the basis of Swiss staining.…”

Section: Cytopathological Specimen Collectionmentioning

confidence: 99%

Minimally Invasive Cytopathology and Accurate Diagnosis: Technical Procedures and Ancillary Techniques

Huang

Wang

et al. 2023

In Vivo

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In recent years, the demand for cytopathological accurate diagnoses has increased as expanding minimally invasive procedures obtain materials from patients with advanced cancer for diagnostic, prognostic, and predictive purposes. However, inadequate knowledge of cytopathological technical procedures and ancillary techniques by clinicians remains the most common reason for the limited availability of cytopathology. The objectives of this review were to understand the technical procedures, ancillary techniques, and application and effectiveness of various types of tests in cytopathology. Each of the many ancillary technologies described in the literature has specific advantages and limitations and laboratories select one or more methods depending on their infrastructure and expertise to achieve the goal from initial screening of the disease to the final diagnosis of the cytopathology. This paper systematically reviews the development of cytopathology, summarizes the existing problems in cytopathology and the new progress of auxiliary examination, to provide a theoretical basis for the advanced development of cytopathological diagnostic technologies and to consolidate the minimally invasive and accurate diagnosis of cytopathologies for clinicians.Cytopathology offers many advantages over other clinical examinations, particularly for minimally invasive and accurate diagnosis.

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Section: Cytopathological Specimen Collectionmentioning

confidence: 99%

Minimally Invasive Cytopathology and Accurate Diagnosis: Technical Procedures and Ancillary Techniques

Huang

Wang

et al. 2023

In Vivo

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“…Past investigations asserted that CellDetect ® has a high sensitivity in detecting neoplastic tissue, including pre-cancerous cells. [12][13][14] The aim of this study is to compare the diagnostic efficacy of Cell Detect ® with urine cytology in patients under bladder cancer follow-up based on cystoscopic evaluations.…”

Section: Introductionmentioning

confidence: 99%

“…Past investigations asserted that CellDetect ® has a high sensitivity in detecting neoplastic tissue, including pre-cancerous cells. 12 –14…”

Section: Introductionmentioning

confidence: 99%

What is the role of CellDetect^® in detecting and monitoring bladder cancer?

et al. 2023

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Purpose: Cystoscopy is the gold standard method for diagnosing and monitoring bladder cancer but it is costly, invasive, and operator-dependant. The aim is to compare the diagnostic efficacy of CellDetect® with urine cytology based on cystoscopic findings. Methods: A total of 181 patients undergoing cystoscopy for bladder cancer follow-up or any reason were studied with cytology and CellDetect® by taking an urine sample before cystoscopy. Patients who had any kind of bladder procedure in less than 1 month, doubtful cystoscopy results, previous pathology of Tis or carcinoma in situ (CIS), urinary stones, and patients with urinary catheters or bladder diversions were excluded. Cytologic and CellDetect® results were compared based on cystoscopic findings and sensitivity and specifity analyses were done for each method. Results: For low-grade tumors, the sensitivity of CellDetect® was 66.7% and the sensitivity of cytology was 16.7% with a significant difference ( p < 0.05). For high-grade tumors, there were no significant difference between CellDetect® and cytology. Generally, CellDetect® had better sensitivity in both case and control groups. Conclusion: The promising results of CellDetect® particularly in low-grade tumors gives the potential for this novel stain to go widespread. Larger series, meta-analyses, and reviews need to support this topic in order to put CellDetect® into daily practice.

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“…Due to its excellent staining characteristics, CellDetect could distinguish normal cells from tumor cells even in a small tumor foci. It has the potential to become one of the most effective examinations for cervical cancer screening and early diagnosis [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Diagnostic value comparison of CellDetect, fluorescent in situ hybridization (FISH), and cytology in urothelial carcinoma

Shang

Liu

et al. 2021

Cancer Cell Int

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Background To evaluate the clinical effectiveness of a novel CellDetect staining technique, compared with fluorescent in situ hybridization (FISH), and urine cytology, in the diagnosis of urothelial carcinoma (UC). Methods A total of 264 patients with suspicious UC were enrolled in this study. All tissue specimens were collected by biopsy or surgery. Urine specimen was obtained for examinations prior to the surgical procedure. CellDetect staining was carried out with CellDetect kit, and FISH was performed with UroVysion detection kit, according to the manufacturer’s instructions. For urine cytology, all specimens were centrifuged using the cytospin method, and the slides were stained by standard Papanicolaou stain. Results In this study, there were 128 cases of UC and 136 cases of non-UC, with no significant difference in gender and age between the two groups. Results for sensitivity of CellDetect, FISH, and urine cytology were 82.8%, 83.6%, and 39.8%, respectively. The specificity of the three techniques were 88.2%, 90.4%, and 86.0%, respectively. The sensitivity of CellDetect and FISH are significantly superior compared to the conventional urine cytology; however, there was no significant difference in specificity among three staining techniques. In addition, the sensitivity of CellDetect in lower urinary tract UC, upper urinary tract UC, non-muscle-invasive bladder cancer (NMIBC), and muscle-invasive bladder cancer (MIBC) were 83.3%, 81.8%, 83.5%, and 72.0%, respectively. The screening ability of CellDetect has no correlation with tumor location and the tumor stage. The sensitivity of CellDetect in low-grade UC and high-grade UC were 51.6 and 92.8%. Thus, screening ability of CellDetect in high-grade UC is significantly superior compared to that in low-grade UC. Conclusions CellDetect and FISH show equal value in diagnosing UC, both are superior to conventional urine cytology. Compared to FISH, CellDetect is cost effective, easy to operate, with extensive clinical application value to monitor recurrence of UC, and to screen indetectable UC.

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Application of the CellDetect® staining technique in diagnosis of human cervical cancer

Abstract: CellDetect® staining technique provided a dual color discrimination and morphological analysis. It has the potential to become one of the most effective methods for cervical screening and early diagnosis.

Cited by 13 publications

References 7 publications

Minimally Invasive Cytopathology and Accurate Diagnosis: Technical Procedures and Ancillary Techniques

Minimally Invasive Cytopathology and Accurate Diagnosis: Technical Procedures and Ancillary Techniques

What is the role of CellDetect^® in detecting and monitoring bladder cancer?

Diagnostic value comparison of CellDetect, fluorescent in situ hybridization (FISH), and cytology in urothelial carcinoma

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Application of the CellDetect® staining technique in diagnosis of human cervical cancer

Abstract: CellDetect® staining technique provided a dual color discrimination and morphological analysis. It has the potential to become one of the most effective methods for cervical screening and early diagnosis.

Cited by 13 publications

References 7 publications

Minimally Invasive Cytopathology and Accurate Diagnosis: Technical Procedures and Ancillary Techniques

Minimally Invasive Cytopathology and Accurate Diagnosis: Technical Procedures and Ancillary Techniques

What is the role of CellDetect® in detecting and monitoring bladder cancer?

Diagnostic value comparison of CellDetect, fluorescent in situ hybridization (FISH), and cytology in urothelial carcinoma

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What is the role of CellDetect^® in detecting and monitoring bladder cancer?