Application of the Biolog EcoPlate™ technique for assessing the microbial metabolic diversity in moss profiles of continental Antarctica

Nagata, Yudai; Osono, Takashi; Hasegawa, Motohiro; Hobara, Satoru; Hirose, Dai; Tanabe, Yukiko; Kudoh, Sakae; Uchida, Masaki

doi:10.1016/j.polar.2023.100924

Cited by 6 publications

(4 citation statements)

References 34 publications

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“…Substrates such as glycogen, α-cyclodextrin and D-cellobiose were utilized by Arctic fjord sediment communities, suggesting the presence of bacteria able to degrade complex compounds [65,70,71]. Metabolic activity on pyruvic acid methyl ester, D-glucosaminic acid, D-galactonic acid-gamma lactone, L-arginine, L-asparagine, and L-serine was also reported in Arctic terrestrial sediment communities [60]; the utilization of D-mannitol, N-acetyl-Dglucosamine, and Tween 80 by both terrestrial and fjord sediment communities suggested that such carbon substrates were readily available in these cold sedimentary environments.…”

Section: Comparison Between Sampling Stations and Among Matricesmentioning

confidence: 87%

“…In east continental Antarctica (coastal outcrops of the Lützow-Holm Bay, Queen Maud Land), the metabolic diversity of the microbial community has recently been explored [60]; the profiles showed that L-arginine, L-asparagine, Tween 40 and 80, D-mannitol and D-galacturonic acid were the most utilized carbon sources.…”

Section: Comparison Between Sampling Stations and Among Matricesmentioning

confidence: 99%

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Microbial Biofilm Colonizing Plastic Substrates in the Ross Sea (Antarctica): First Overview of Community-Level Physiological Profiles

Caruso

Maimone

Rappazzo

et al. 2023

JMSE

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The microbial colonization of plastic substrates made of polyvinylchloride (PVC) and polyethylene (PE) was studied in Tethys and Road Bays (Ross Sea, Antarctica) in order to evaluate the metabolic profiles of the plastisphere community in comparison with those of the surrounding waters. PVC and PE panels, mounted on stainless steel structures, were deployed in the austral summer 2017 at 5 and 20 m and recovered one year later at four different stations (Amorphous Glacier-AG was potentially impacted by the ice-melting process, and its control site was within Tethys Bay-TB; Road Bay-RB, close to the wastewater plant of the Italian research station Mario Zucchelli and its control site Punta Stocchino-PTS). Additional panels were settled in Road Bay at 5 m and recovered after three months to follow time variability in the microbial colonization process. At the same times and depths as plastic substrates, water samples were also collected. Carbon substrates’ utilization rates were determined on scraped microbial biofilm and water samples, with a fluorimetric assay based on 96-well Biolog Ecoplates. Complex carbon sources, carbohydrate and amines were the organic substrates that mostly fuelled the community metabolism in the RB area, while in the TB area, in addition to carbohydrates, phosphate carbon compounds and amino acids were also actively utilized. Within Road Bay, small differences in the physiological profiles were found, with higher metabolic rates in the biofilm community after 3 months’ deployment (late austral summer period) compared to 12 months, suggesting that autumn to spring period conditions negatively affected foulers’ metabolism. Moreover, different metabolic profiles between the plastisphere and the pelagic microbial community were observed; this last utilized a higher number of carbon sources, while plastic substrates were colonized by a more specialized community. Higher carbon substrate utilization rates were recorded at RB and AG stations, receiving organic supply from anthropic activity or ice melting sources, respectively, compared to their control sites. These results highlighted the functional plasticity of the microbial community, with the adaptive ability to utilize a diversified range of organic substrates.

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Section: Comparison Between Sampling Stations and Among Matricesmentioning

confidence: 87%

Section: Comparison Between Sampling Stations and Among Matricesmentioning

confidence: 99%

Microbial Biofilm Colonizing Plastic Substrates in the Ross Sea (Antarctica): First Overview of Community-Level Physiological Profiles

Caruso

Maimone

Rappazzo

et al. 2023

JMSE

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“…Then, the utilization pattern was observed by the reduction of tetrazolium dye, which switches from colourless to purple during the growth of microbes. The results have been expressed as average well colour development (AWCD) = ΣODi/31 22 , 24 , 25 . Mean optical density for the time-dependant utilization pattern of each substrate as well as different diversity indices for heterogeneity i.e., richness and evenness of microbes for functional differences in samples.…”

Section: Methodsmentioning

confidence: 99%

High-altitude and low-altitude adapted chicken gut-microbes have different functional diversity

Bhagat,

Chauhan,

Verma

et al. 2023

Sci Rep

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Recently, there has been considerable interest in the functions of gut microbiota in broiler chickens in relation to their use as feed additives. However, the gut-microbiota of chickens reared at different altitudes are not well documented for their potential role in adapting to prevailing conditions and functional changes. In this context, the present study investigates the functional diversity of gut-microbes in high-altitude (HACh) and low-altitude adapted chickens (LACh), assessing their substrate utilization profile through Biolog Ecoplates technology. This will help in the identification of potential microbes or their synthesized metabolites, which could be beneficial for the host or industrial applications. Results revealed that among the 31 different types of studied substrates, only polymers, carbohydrates, carboxylic acids, and amine-based substrates utilization varied significantly (p < 0.05) among the chickens reared at two different altitudes where gut-microbes of LACh utilized a broad range of substrates than the HACh. Further, diversity indices (Shannon and MacIntosh) analysis in LACh samples showed significant (p < 0.05) higher richness and evenness of microbes as compared to the HACh samples. However, no significant difference was observed in the Simpson diversity index in gut microbes of lowversus high-altitude chickens. In addition, the Principal Component Analysis elucidated variation in substrate preferences of gut-microbes, where 13 and 8 carbon substrates were found to constitute PC1 and PC2, respectively, where γ-aminobutyric acid, d-glucosaminic acid, i-erythritol and tween 40 were the most relevant substrates that had a major effect on PC1, however, alpha-ketobutyric acid and glycyl-l-glutamic acid affected PC2. Hence, this study concludes that the gut-microbes of high and low-altitudes adapted chickens use different carbon substrates so that they could play a vital role in the health and immunity of an animal host based on their geographical location. Consequently, this study substantiates the difference in the substrate utilization and functional diversity of the microbial flora in chickens reared at high and low altitudes due to altitudinal changes.

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“…A crucial concept for differentiating and classifying microbial species according to their capacity to metabolize various carbon sources is that different microorganisms often have distinct carbon source metabolism profiles [29][30][31][32]. With this knowledge, different microorganisms can be used for the pre-treatment of soy molasses to break down sucrose and utilize it through the microorganisms' specific carbon source metabolism while preserving FSO such as raffinose and stachyose to the greatest extent possible.…”

Section: Introductionmentioning

confidence: 99%

The Identification of a Strain for the Biological Purification of Soy Molasses to Produce Functional Soy Oligosaccharides and Optimize Purification Conditions

Yang,

Ma,

Wang

et al. 2024

Foods

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Soy molasses is rich in oligosaccharides like sucrose, stachyose, and raffinose, with stachyose and raffinose being functional oligosaccharides. Harnessing soy molasses for the production of functional soy oligosaccharides (FSO) can significantly elevate its value. Biological purification, a method leveraging the selective utilization of different carbon sources by microorganisms, allows for the specific removal of sucrose from soy molasses while preserving stachyose and raffinose, thereby increasing the FSO content. This research identified a yeast named YT312 with strong purification capabilities for soy molasses and optimized the purification conditions. The study revealed that yeast YT312 was Wickerhamomyces anomalus, exhibiting a broad range of growth temperatures and pH levels alongside a high tolerance to glucose, sucrose, and NaCl. Through single-factor and orthogonal experiments, it was established that under specific conditions—0.375% inoculum size, 30 °C fermentation temperature, 150 rpm shaking speed, 10-fold dilution ratio, pH of 7, and 12 h of fermentation—sucrose was completely removed from soy molasses, while functional raffinose and stachyose were retained at rates of 96.1% and 90.2%, respectively. Consequently, W. anomalus YT312 displayed exceptional characteristics for the biological purification of soy molasses and the production of FSO.

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Application of the Biolog EcoPlate™ technique for assessing the microbial metabolic diversity in moss profiles of continental Antarctica

Cited by 6 publications

References 34 publications

Microbial Biofilm Colonizing Plastic Substrates in the Ross Sea (Antarctica): First Overview of Community-Level Physiological Profiles

Microbial Biofilm Colonizing Plastic Substrates in the Ross Sea (Antarctica): First Overview of Community-Level Physiological Profiles

High-altitude and low-altitude adapted chicken gut-microbes have different functional diversity

The Identification of a Strain for the Biological Purification of Soy Molasses to Produce Functional Soy Oligosaccharides and Optimize Purification Conditions

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