Application of the 2,2‘-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Radical Cation Assay to a Flow Injection System for the Evaluation of Antioxidant Activity of Some Pure Compounds and Beverages

Pellegrini, Nicoletta; Rio, Daniele Del; Colombi, Barbara; Bianchi, Marta; Brighenti, Furio

doi:10.1021/jf020657z

Cited by 131 publications

(100 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The setting potential was 0, 100, 200, 300 and 400 mV (v. Palladium reference electrode) and the chromatographic separation was obtained applying an isocratic elution at flow rate of 0.8 ml/min. Total Antioxidant Capacity, carotenoids, polyphenols and total vitamin C were measured as follow: total antioxidant capacity (TAC) using two different assays Ferric Reducing Antioxidant Power (FRAP) (Benzie & Strain, 1996) and Trolox Equivalent Antioxidant Capacity (TEAC) method (Pellegrini et al, 2003;Re et al, 1999).…”

Section: Methodsmentioning

confidence: 99%

Phytochemicals Content in Italian Garlic Bulb (Allium sativum L.) Varieties

Azzini¹,

Durazzo²,

Foddai³

et al. 2014

JFR

View full text Add to dashboard Cite

Several studies have demonstrated a wide range of therapeutic effects due to garlic high content of phytochemicals, including sulfur-containing compounds, vitamins, saponins, flavonoids and moderate levels of carotenoids. The synergistic interactions between these components seem to explain the outcomes of certain healing properties from garlic. This study evaluates the health promoting phytochemicals (ascorbic acid, flavonoids and carotenoids) content and antioxidant capacity in four "typical garlic varieties" (Rosso di Castelliri, Bianco Piacentino, Rosso di Sulmona, Rosso di Proceno), grown in different geographical areas. -carotene content ranged from 5.68 to 7.41 g/100g and 6.36 to 7.46 g/100g for Viterbo and Alvito bulbs respectively. Overall, vitamin C levels were statistically higher in samples from Alvito compared with same cultivars from Viterbo; among them Rosso Sulmona and Rosso Castelliri displayed the higher content (21.59 ± 2.75 and 18.91 ± 0.34 mg/100 g respectively). FRAP values were positively correlated (r = 0.74 and P = 0.03) with vitamin C levels and highly correlated (r = 0.86 and P = 0.005) with myricetin levels. Our findings have revealed that genotype and environmental conditions (production areas and pedoclimatic factors), as well as their interaction, could influence the phytochemical composition and the antioxidant properties of Italian garlic bulb varieties.

show abstract

Section: Methodsmentioning

confidence: 99%

Phytochemicals Content in Italian Garlic Bulb (Allium sativum L.) Varieties

Azzini¹,

Durazzo²,

Foddai³

et al. 2014

JFR

View full text Add to dashboard Cite

show abstract

“…Subsequently, methanol extracts (5 g of raw vegetables in 80 mL of a 70% methanol solution) were obtained from a mean representative sample by shaking the fresh plant material in an Elpan, 357 water bath shaker at ambient temperature for 2 h. They were then centrifuged using a MPW-340 centrifuge, fi ltered and fi nally stored at -22°C [Pellegrini et al, 2003].…”

Section: Preparation Of Methanol Extractsmentioning

confidence: 99%

Impact of Different Packaging Systems on Selected Antioxidant Properties of Frozen-Stored Cauliflower (Brassica oleracea L. var. botrytis)

Kapusta‐Duch¹,

Leszczyńska²,

Borczak³

et al. 2017

Pol. J. Food Nutr. Sci.

View full text Add to dashboard Cite

“…Data on TAC were included in the data bank on a number of foods directly analyzed in our laboratory and representative of the average Northern Italian diet (Pellegrini et al, 2003a;Pellegrini et al, 2006). Dietary TAC values used in this study have been obtained by applying the Trolox equivalents antioxidant capacity assay (Pellegrini et al, 2003b), expressed as TAC in mmol of Trolox/kg of fresh food, and reported as mmol of Trolox/day.…”

Section: Dietary Datamentioning

confidence: 99%

The total antioxidant capacity of the diet is an independent predictor of plasma β-carotene

et al. 2006

View full text Add to dashboard Cite

Objective: To investigate the contribution of the total antioxidant capacity (TAC) of the diet to plasma concentrations of b-carotene. Design: Cross-sectional study. Setting: Department of Public Health and Department of Internal Medicine and Biomedical Sciences, University of Parma. Subjects: A total of 247 apparently healthy adult men (n ¼ 140) and women (n ¼ 107). Methods: A medical history, a physical exam including height, weight, waist circumference and blood pressure measurements, a fasting blood draw, an oral glucose tolerance test and a 3-day food record. Results: We observe a negative trend across quartiles of plasma b-carotene for most biological variables clustering in the insulin resistance syndrome, as well as for traditional and new risk factors for type II diabetes and cardiovascular disease (CVD), including C-reactive protein and g-glutamyltranspeptidase (Po0.05). Regarding dietary characteristics, energy-adjusted intake of fat, fiber, fruits, vegetables, b-carotene, vitamin C, vitamin E and dietary TAC significantly increased with increasing plasma b-carotene (Po0.05), whereas alcohol intake decreased (P ¼ 0.013). Adjusted geometric means (95% confidence interval) of plasma b-carotene significantly increased across quartiles of dietary TAC, even when single dietary antioxidants were considered in the model (QI ¼ 0.087 mg/dl (0.073-0.102); QII ¼ 0.087 mg/dl (0.075-0.103); QIII ¼ 0.114 mg/dl (0.098-0.132) and QIV ¼ 0.110 mg/dl (0.093-0.130); P for linear trend ¼ 0.026). When the population was divided on the basis of alcohol consumption, this trend was also observed in subjects drinking o20 g alcohol/day (P ¼ 0.034), but not in those with higher alcohol intake (P ¼ 0.448). Conclusions: Dietary TAC is an independent predictor of plasma b-carotene, especially in moderate alcohol drinkers. This may explain, at least in part, the inverse relationship observed between plasma b-carotene and risk of chronic diseases associated to high levels of oxidative stress (i.e., diabetes and CVD), as well as the failure of b-carotene supplements alone in reducing such risk. Contributors: SV participated in the study design and the collection of clinical data, performed the statistical analysis and was the primary writer of the paper, but received input from all the other authors. DDR contributed to the preparation of the paper, to laboratory analysis and to the study design. NP provided expert advice and was involved in paper editing and data interpretation. DA and LF participated in subjects' recruitment and management, in the collection and interpretation of clinical data and in the discussion of the paper. PMP and SS were involved in laboratory analysis and quality control. PR carried out the analysis of plasma b-carotene and provided expert advice on data interpretation. DDR, NP, SS and FB were responsible for the collection, completeness and interpretation of dietary data. IZ was responsible for ethical approval and subjects' recruitment. IZ and FB were responsible for the study concept and design and for sec...

show abstract

Application of the 2,2‘-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Radical Cation Assay to a Flow Injection System for the Evaluation of Antioxidant Activity of Some Pure Compounds and Beverages

Cited by 131 publications

References 24 publications

Phytochemicals Content in Italian Garlic Bulb (Allium sativum L.) Varieties

Phytochemicals Content in Italian Garlic Bulb (Allium sativum L.) Varieties

Impact of Different Packaging Systems on Selected Antioxidant Properties of Frozen-Stored Cauliflower (Brassica oleracea L. var. botrytis)

The total antioxidant capacity of the diet is an independent predictor of plasma β-carotene

Contact Info

Product

Resources

About