Application of synthetic peptides to the diagnosis of neurocysticercosis

Fleury, Agnès; Beltrán, Carmen; Ferrer, Elizabeth; Gárate, Teresa; Harrison, Leslie J.; Parkhouse, R. M. E.; Garcı́a, Esperanza; Fragoso, Gladis; Costa-Cruz, Júlia Maria; Biondi, Germano Francisco; Agapejev, Svetlana; Sciutto, Edda

doi:10.1046/j.1360-2276.2003.01132.x

Cited by 31 publications

(27 citation statements)

References 17 publications

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“…Also, peptides derived from T. saginata oncosphere molecules have been used for cysticercosis diagnosis and human and animal disease, with similar results to the ones reported above (Fleury et al 2003;Ferrer et al 2005b). …”

Section: Immunodiagnosissupporting

confidence: 65%

Taeniosis and Cysticercosis

Ferrer

Gárate

2014

Helminth Infections and Their Impact on Global Public Health

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Taeniosis and cysticercosis are zoonotic diseases produced by Taenia saginata and Taenia solium. The adult tapeworms are parasites of human intestine and show a wide geographical distribution. Taenia asiatica, another tapeworm species, was described in Southeast Asia. The larval stages of these cestodes (metacestodes or cysticerci) cause cysticercosis; T. saginata causes bovine cysticercosis, T. asiatica larvae develop in the pig viscera, and T. solium is able to produce cysticercosis in both pig and man. When the parasite larva invades, the central nervous system (CNS) can provoke neurocysticercosis (NCC), one of the most frequent parasitic infections of human CNS. These diseases continue to cause health problems and livestock industry losses in areas where the parasites are endemic and also in non-endemic regions as a consequence of travel and migrations. There are few symptoms associated with taeniosis; in contrast, NCC (pleomorphic pathology) could be a life-threatening disease, depending on the location, number, stage of cysticerci, and the host immune response. Diagnosis of taeniosis is generally achieved by stool microscopic examinations, and the detection of cysticercosis is generally performed by neuroimaging and immunoassays. Both conventional coprological techniques and immunological assays show limitations, and new diagnostic tools have been developed, more specific and sensitive, such as specific monoclonal antibodies, recombinant antigens, synthetic peptides, and PCR. Considering the clinical impact, veterinary problems, and economic losses derived from taeniosis/cysticercosis, control programs have been implemented. In addition, several vaccine candidates have been characterized that could complement the control measures.

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Section: Immunodiagnosissupporting

confidence: 65%

Taeniosis and Cysticercosis

Ferrer

Gárate

2014

Helminth Infections and Their Impact on Global Public Health

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“…However, to date no synthetic peptide has performed at the level of native antigens for diagnostic purposes. 61,67,[79][80][81] A possible alternative is to use more than one synthetic peptide in the same assay, 82 as in the multiantigen print immunoassay, in which several recombinant or synthetic antigens are printed at different positions along a single strip and thus obviating the need for electrophoretic separation. 83 Recombinant proteins perform better than synthetic peptides, most likely because part of the response is directed towards conformational epitopes.…”

Section: -24mentioning

confidence: 99%

Immunological and molecular diagnosis of cysticercosis

Rodríguez¹,

Wilkins

Dorny

2012

Pathogens and Global Health

133

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Cysticercosis, the infection with the larval stage of Taenia solium, is a cause of neurological symptoms including seizures, affecting the quality of life of patients and their families. Diagnosis focuses on brain imaging and serological tests are mostly used as confirmatory tools. Most cases, however, occur in poor endemic areas, where both kinds of diagnostic tools are poorly available. Development of point of care diagnostic tests is one of the most important priorities for cysticercosis researches today. The ideal point of care test would require detection of viable cysticercosis and hopefully identify cases with severe or progressive forms of neurocysticercosis, leading to referral of the patient for specialized medical attention. This manuscript describes the evolution of the serological diagnosis of cysticercosis over time, and the characteristics of the most common currently available tools, their advantages and disadvantages, and their potential use in future diagnostic tests.

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“…Es necesario emprender nuevos estudios que permitan validar la prueba con extractos antigénicos semipurificados o purificados, recombinantes o sintéticos, para así mejorar los niveles de sensibilidad obtenidos, tal como lo reportan otros investigadores (27)(28)(29)(30). Un estudio (31) que compara el EP con el EITB usando un antígeno purificado para Parastrongylus cantonensis, concluye que las pruebas son comparables en sus niveles de sensibilidad y especificidad.…”

Section: Discussionunclassified