Application of Surface-Enhanced Raman Spectroscopy for Detection of Beta Amyloid Using Nanoshells

Beier, Hope T.; Cowan, Christopher B.; Chou, I-Hsien; Pallikal, James; Henry, James; Benford, Melodie; Jackson, J. B.; Good, Theresa A.; Coté, Gerard L.

doi:10.1007/s11468-007-9027-x

Cited by 69 publications

(43 citation statements)

References 61 publications

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“…Several types of SERS‐based sensing methods have been developed to detect plasma AD biomarkers Aβ40 and Aβ42 . However, these methods appear to be insufficient in terms of detection sensitivity for the quantification of low levels of Aβ40 and Aβ42 in blood.…”

Section: Introductionmentioning

confidence: 99%

Reaction Kinetics‐Mediated Control over Silver Nanogap Shells as Surface‐Enhanced Raman Scattering Nanoprobes for Detection of Alzheimer's Disease Biomarkers

Yang

Hwang

Geun

et al. 2019

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It is very challenging to accurately quantify the amounts of amyloid peptides Aβ40 and Aβ42, which are Alzheimer's disease (AD) biomarkers, in blood owing to their low levels. This has driven the development of sensitive and noninvasive sensing methods for the early diagnosis of AD. Here, an approach for the synthesis of Ag nanogap shells (AgNGSs) is reported as surface‐enhanced Raman scattering (SERS) colloidal nanoprobes for the sensitive, selective, and multiplexed detection of Aβ40 and Aβ42 in blood. Raman label chemicals used for SERS signal generation modulate the reaction rate for AgNGSs production through the formation of an Ag‐thiolate lamella structure, enabling the control of nanogaps at one nanometer resolution. The AgNGSs embedded with the Raman label chemicals emit their unique SERS signals with a huge intensity enhancement of up to 107 and long‐term stability. The AgNGS nanoprobes, conjugated with an antibody specific to Aβ40 or Aβ42, are able to detect these AD biomarkers in a multiplexed manner in human serum based on the AgNGS SERS signals. Detection is possible for amounts as low as 0.25 pg mL−1. The AgNGS nanoprobe‐based sandwich assay has a detection dynamic range two orders of magnitude wider than that of a conventional enzyme‐linked immunosorbent assay.

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Section: Introductionmentioning

confidence: 99%

Reaction Kinetics‐Mediated Control over Silver Nanogap Shells as Surface‐Enhanced Raman Scattering Nanoprobes for Detection of Alzheimer's Disease Biomarkers

Yang

Hwang

Geun

et al. 2019

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“…In order to obtain specific interactions with amyloid structures, nanoparticles have been functionalized with molecules of various affinities, such as a peptide,26 sialic acid,29, 30 thioflavin,27 a stilbene derivative,31 and curcumin 32–43. Among these, curcumin is one of the most studied molecules and acts as a very effective molecular chaperone in relation to various human neurological disorders and is known to inhibit amyloid fibrillation and to disintegrate amyloid fibrils 32–43.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Amyloid Fibril Growth and Dissolution of Amyloid Fibrils by Curcumin–Gold Nanoparticles

Palmal

Maity

Singh

et al. 2014

Chemistry A European J

143

138

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Inhibition of amyloid fibrillation and clearance of amyloid fibrils/plaques are essential for the prevention and treatment of various neurodegenerative disorders involving protein aggregation. Herein, we report curcumin-functionalized gold nanoparticles (Au-curcumin) of hydrodynamic diameter 10-25 nm, which serve to inhibit amyloid fibrillation and disintegrate/dissolve amyloid fibrils. In nanoparticle form, curcumin is water-soluble and can efficiently interact with amyloid protein/peptide, offering enhanced performance in inhibiting amyloid fibrillation and dissolving amyloid fibrils. Our results imply that nanoparticle-based artificial molecular chaperones may offer a promising therapeutic approach to combat neurodegenerative disease.

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“…We use the amyloid β‐protein (Aβ), a well‐studied pathologic agent of Alzheimer's disease, as an example biologically relevant analyte to assess the potential of the hybrid platform for quantification and subsequent study of Aβ assembly dynamics. We observe two complementary quantification modes.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive amyloid β‐protein quantification with high dynamic range using a hybrid graphene–gold surface‐enhanced Raman spectroscopy platform

Hayden

Wang

et al. 2019

J Raman Spectroscopy

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Surface enhanced Raman spectroscopy (SERS) holds great promise in biosensing because of its single‐molecule, label‐free sensitivity. We describe here the use of a graphene–gold hybrid plasmonic platform that enables quantitative SERS measurement. Quantification is enabled by normalizing analyte peak intensities to that of the graphene G peak. We show that two complementary quantification modes are intrinsic features of the platform and that through their combined use, the platform enables accurate determination of analyte concentration over a concentration range spanning seven orders of magnitude. We demonstrate, using a biologically relevant test analyte, the amyloid β‐protein (Aβ), a seminal pathologic agent of Alzheimer's disease, that linear relationships exist between (a) peak intensity and concentration at a single plasmonic hot spot smaller than 100 nm and (b) frequency of hot spots with observable protein signals, that is, the colocation of an Aβ protein and a hot spot. We demonstrate the detection of Aβ at a concentration as low as 10−18 M after a single 20 μl aliquot of the analyte onto the hybrid platform. This detection sensitivity can be improved further through multiple applications of analyte to the platform and by rastering the laser beam with smaller step sizes.

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Application of Surface-Enhanced Raman Spectroscopy for Detection of Beta Amyloid Using Nanoshells

Cited by 69 publications

References 61 publications

Reaction Kinetics‐Mediated Control over Silver Nanogap Shells as Surface‐Enhanced Raman Scattering Nanoprobes for Detection of Alzheimer's Disease Biomarkers

Reaction Kinetics‐Mediated Control over Silver Nanogap Shells as Surface‐Enhanced Raman Scattering Nanoprobes for Detection of Alzheimer's Disease Biomarkers

Inhibition of Amyloid Fibril Growth and Dissolution of Amyloid Fibrils by Curcumin–Gold Nanoparticles

Ultrasensitive amyloid β‐protein quantification with high dynamic range using a hybrid graphene–gold surface‐enhanced Raman spectroscopy platform

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