Application of surface chemical analysis tools for characterization of nanoparticles

Baer, Donald R.; Gaspar, Daniel J.; Nachimuthu, Ponnusamy; Techane, Sirnegeda D.; Castner, David G.

doi:10.1007/s00216-009-3360-1

Cited by 256 publications

(206 citation statements)

References 101 publications

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“…secondary ion mass spectrometry and low energy ion scattering) and other spectroscopic techniques (i.e. IR, NMR, Raman) (Baer, Gaspar, Nachimuthu, Techane, & Castner, 2010). The most important advantage of electron spectroscopy is its high surface sensitivity.…”

Section: Surface Functionalization (Eg Coating or Modification)mentioning

confidence: 99%

(Q)SAR modelling of nanomaterial toxicity: A critical review

et al. 2015

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There is an increasing recognition that nanomaterials pose a risk to human health, and that the novel engineered nanomaterials (ENMs) in the nanotechnology industry and their increasing industrial usage poses the most immediate problem for hazard assessment, as many of them remain untested. The large number of materials and their variants (different sizes and coatings for instance) that require testing and ethical pressure towards non-animal testing means that expensive animal bioassay is precluded, and the use of (quantitative) structure activity relationships ((Q)SAR) models as an alternative source of hazard information should be explored.(Q)SAR modelling can be applied to fill the critical knowledge gaps by making the best use of existing data, prioritize physicochemical parameters driving toxicity, and provide practical solutions to the risk assessment problems caused by the diversity of ENMs. This paper covers the core components required for successful application of (Q)SAR technologies to ENMs toxicity prediction, and summarizes the published nano-(Q)SAR studies and outlines the challenges ahead for nano-(Q)SAR modelling. It provides a critical review of (1) the present status of the availability of ENMs characterization/toxicity data, (2) the characterization of nanostructures that meets the need of (Q)SAR analysis, (3) the summary of published nano-(Q)SAR studies and their limitations, (4) the in silico tools for (Q)SAR screening of nanotoxicity and (5) the prospective directions for the development of nano-(Q)SAR models.

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Section: Surface Functionalization (Eg Coating or Modification)mentioning

confidence: 99%

(Q)SAR modelling of nanomaterial toxicity: A critical review

et al. 2015

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“…Some peak broadening at ∼708.5 eV suggests the appearance of a new Fe(II) component in the "shell" [31]. Combined with the decrease of Fe(III) on the surface, a likely explanation for the increasing Fe(II) content is that Fe(OH) 3 is reduced to Fe(OH) 2 by zerovalent iron (ZVI).…”

Section: Tem Analysis On the Core-shell Structure Of Nzvimentioning

confidence: 99%

A new insight on the core–shell structure of zerovalent iron nanoparticles and its application for Pb(II) sequestration

Zhang

Zhou

et al. 2013

Journal of Hazardous Materials

131

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“…A number of research groups have used a variety of techniques to detect the presence of these particles in the environment, including transmission electron microscopy (7), scanning electron microscopy (8), and atomic force microscopy (9)(10)(11). Suspensions of nanoparticles can be assessed using dynamic light scattering (DLS) to yield a hydrodynamic diameter.…”

mentioning

confidence: 99%

Detection of TiO₂ nanoparticles in cells by flow cytometry

et al. 2010

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Evaluation of the potential hazard of man-made nanomaterials has been hampered by a limited ability to observe and measure nanoparticles in cells. In this study, different concentrations of TiO 2 nanoparticles were suspended in cell culture medium. The suspension was then sonicated and characterized by dynamic light scattering and microscopy. Cultured human-derived retinal pigment epithelial cells (ARPE-19) were incubated with TiO 2 nanoparticles at 0, 0.1, 0.3, 1, 3, 10, and 30 lg/ml for 24 hours. Cellular reactions to nanoparticles were evaluated using flow cytometry and dark field microscopy. A FACSCalibur TM flow cytometer was used to measure changes in light scatter after nanoparticle incubation. Both the side scatter and forward scatter changed substantially in response to the TiO 2 . From 0.1 to 30 lg/ml TiO 2 , the side scatter increased sequentially while the forward scatter decreased, presumably due to substantial light reflection by the TiO 2 particles. Based on the parameters of morphology and the calcein-AM/propidium iodide viability assay, TiO 2 concentrations below 30 lg/ml TiO 2 caused minimal cytotoxicity. Microscopic analysis was done on the same cells using an E-800 Nikon microscope containing a xenon light source and special dark field objectives. At the lowest concentrations of TiO 2 (0.1-0.3 lg/ml), the flow cytometer could detect as few as 5-10 nanoparticles per cell due to intense light scattering by TiO 2 . Rings of concentrated nanoparticles were observed around the nuclei in the vicinity of the endoplasmic reticulum at higher concentrations. These data suggest that the uptake of nanoparticles within cells can be monitored with flow cytometry and confirmed by dark field microscopy. This approach may help fulfill a critical need for the scientific community to assess the relationship between nanoparticle dose and cellular toxicity Such experiments could potentially be performed more quickly and easily using the flow cytometer to measure both nanoparticle uptake and cellular health. Published

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Application of surface chemical analysis tools for characterization of nanoparticles

Cited by 256 publications

References 101 publications

(Q)SAR modelling of nanomaterial toxicity: A critical review

(Q)SAR modelling of nanomaterial toxicity: A critical review

A new insight on the core–shell structure of zerovalent iron nanoparticles and its application for Pb(II) sequestration

Detection of TiO₂ nanoparticles in cells by flow cytometry

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Application of surface chemical analysis tools for characterization of nanoparticles

Cited by 256 publications

References 101 publications

(Q)SAR modelling of nanomaterial toxicity: A critical review

(Q)SAR modelling of nanomaterial toxicity: A critical review

A new insight on the core–shell structure of zerovalent iron nanoparticles and its application for Pb(II) sequestration

Detection of TiO2 nanoparticles in cells by flow cytometry

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Product

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Detection of TiO₂ nanoparticles in cells by flow cytometry