Application of surface acoustic waves for optimisation of biocompatibility of carboxymethylated dextran surfaces

Marković, Goran; Mutschler, T.; Wöllner, Karin; Gauglitz, Günter

doi:10.1016/j.surfcoat.2006.01.062

Cited by 17 publications

(4 citation statements)

References 22 publications

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“…However, in many cases, especially in complex matrixes such as blood, PEG better reduces nonspecific binding. Some more biopolymer layers are known, such as biotin-(strept)avidin biolayers, polyelectrolyte layers, histidine tags or membranes, or even biomimetic membranes (24)(25)(26)(27). In Reference 28, applications of hydrogel films in biosensing are reviewed.…”

Section: Setupmentioning

confidence: 99%

Point-of-Care Platforms

Gauglitz

2014

Annual Rev. Anal. Chem.

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Point-of-care applications are gaining increasing interest in clinical diagnostics and emergency applications. Biosensors are used to monitor the biomolecular interaction process between a disease biomarker and a recognition element such as a reagent. Essential are the quality and selectivity of the recognition elements and assay types used to improve sensitivity and to avoid nonspecific interactions. In addition, quality measures are influenced by the detection principle and the evaluation strategies. For these reasons, this review provides a survey and validation of recognition elements, assays, and various types of detection methods for point-of-care testing (POCT) platforms. Common applications of clinical parameters are discussed and considered. In this ever-changing field, a snapshot of current applications is needed. We provide such a snapshot by way of a table including literature citations and also discuss these applications in more detail throughout.

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Section: Setupmentioning

confidence: 99%

Point-of-Care Platforms

Gauglitz

2014

Annual Rev. Anal. Chem.

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“…Cross-linking is needed to prevent them from breaking down in water but they are still mechanically weak. Also, these coatings tend to be porous and sometimes show non-selective adsorption (Markovic 2006).…”

Section: Natural Polymersmentioning

confidence: 99%

Magnetic nanoparticles for gene and drug delivery

McBain

Yiu²,

Dobson

2008

IJN

234

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Abstract:Investigations of magnetic micro-and nanoparticles for targeted drug delivery began over 30 years ago. Since that time, major progress has been made in particle design and synthesis techniques, however, very few clinical trials have taken place. Here we review advances in magnetic nanoparticle design, in vitro and animal experiments with magnetic nanoparticle-based drug and gene delivery, and clinical trials of drug targeting.

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“…Organic–inorganic hybrids have excellent potential as support materials for enzymes providing enhanced stability, mechanical resistance and compatibility with biological molecules. These support materials comprise inorganic materials, such as silica, inorganic oxides, minerals, carbon materials and magnetic nanoparticles coated with polymers or polysaccharides, such as chitosan [ 12 ], poly(ethylene glycol) (PEG) [ 13 ], cellulose [ 14 ] or dextran [ 15 ]. Polysaccharides are effective for the reduction of protein and silica surface interactions and for suppressing unwanted non-specific binding due to increased hydrophilicity of the carrier [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Immunoaffinity extraction followed by enzymatic digestion for the isolation and identification of proteins employing automated μSPE reactors and mass spectrometry

et al. 2022

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This work describes a novel automated and rapid method for bottom-up proteomics combining protein isolation with a micro-immobilised enzyme reactor (IMER). Crosslinking chemistry based on 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling was exploited to immobilise trypsin and antibodies onto customisable silica particles coated with carboxymethylated dextran (CMD). This novel silica–CMD solid-phase extraction material was characterised using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), conductometric titrations and enzymatic colorimetric assays. Micro-solid-phase extraction (μSPE) cartridges equipped with the modified CMD material were employed and integrated into an automated and repeatable workflow using a sample preparation workstation to achieve rapid and repeatable protein isolation and pre-concentration, followed by tryptic digestion producing peptide fragments that were identified by liquid chromatography mass spectrometry (LC-MS). Graphical abstract

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Application of surface acoustic waves for optimisation of biocompatibility of carboxymethylated dextran surfaces

Cited by 17 publications

References 22 publications

Point-of-Care Platforms

Point-of-Care Platforms

Magnetic nanoparticles for gene and drug delivery

Immunoaffinity extraction followed by enzymatic digestion for the isolation and identification of proteins employing automated μSPE reactors and mass spectrometry

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