Application of 16O/18O reverse proteolytic labeling to determine the effect of biofilm culture on the cell envelope proteome of Porphyromonas gingivalis W50

C, Ang; Veith, Paul D.; Dashper, Stuart G.; Reynolds, Eric C.

doi:10.1002/pmic.200700557

Cited by 50 publications

(61 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(a) Electron micrograph of P. gingivalis and T. denticola growing in continuous co-culture, showing the intimate association of the bacteria and the putative vesicles on the surface of the treponeme (arrows). The bacteria were co-cultured under conditions similar to those described previously for the culture of T. denticola in Oral Bacterial Growth Medium (Orth et al, 2010) and P. gingivalis (Ang et al, 2008). T. denticola was initially inoculated into the chemostat, and once cell density had stabilized, P. gingivalis was added to the continuous culture.…”

Section: Biofilmsmentioning

confidence: 99%

Virulence Factors of the Oral Spirochete Treponema denticola

et al. 2010

View full text Add to dashboard Cite

There is compelling evidence that treponemes are involved in the etiology of several chronic diseases, including chronic periodontitis as well as other forms of periodontal disease. There are interesting parallels with other chronic diseases caused by treponemes that may indicate similar virulence characteristics. Chronic periodontitis is a polymicrobial disease, and recent animal studies indicate that co-infection of Treponema denticola with other periodontal pathogens can enhance alveolar bone resorption. The bacterium has a suite of molecular determinants that could enable it to cause tissue damage and subvert the host immune response. In addition to this, it has several non-classic virulence determinants that enable it to interact with other pathogenic bacteria and the host in ways that are likely to promote disease progression. Recent advances, especially in molecular-based methodologies, have greatly improved our knowledge of this bacterium and its role in disease.

show abstract

Section: Biofilmsmentioning

confidence: 99%

Virulence Factors of the Oral Spirochete Treponema denticola

et al. 2010

View full text Add to dashboard Cite

show abstract

“…1), or due to reduced growth rates in the biofilm [115]. This is in contrast to findings in a monospecies biofilm, which would have a similar growth rate to a polymicrobial biofilm, where HmuY was more abundant than in planktonic cells [116]. Commensurate with this polymicrobial approach Mashburn et al [117] have shown that Pseudomonas aeruginosa relies on Staphylococcus aureus as an iron source in vivo.…”

Section: Metal Acquisition Systems Of P Gingivalismentioning

confidence: 92%

The interplay between iron, haem and manganese in Porphyromonas gingivalis

Butler

Dashper

Khan

et al. 2015

Journal of Oral Biosciences

Self Cite

View full text Add to dashboard Cite

a b s t r a c tBackground: Transition metals including iron and manganese are necessary for life because of their ability to donate and accept electrons. Approximately one-third of all proteins require essential transition metal ions to perform catalytic, structural and regulatory functions. These essential metal ions react differently to the presence of oxygen radicals with iron directly involved in the formation of toxic reactive oxygen species, whilst manganese can protect against oxidative stress. Highlight: Anaerobic bacterial species have been poorly studied with regard to transition metal homoeostasis and behave differently in many respects when compared with aerobic or aerotolerant species. To optimise catabolism whilst protecting themselves from unwanted reactions bacterial cells must maintain intracellular metal levels in a very narrow range that varies, dependent on the environment. To maintain metal ion homoeostasis, bacteria have evolved complex regulatory mechanisms of metal uptake, secretion and storage. In this review we examine how iron, haem and manganese availability dictate the lifestyle and virulence of the anaerobic Gram-negative, periodontal pathogen Porphyromonas gingivalis. Conclusion: P. gingivalis has novel haem, iron and manganese transporters and metalloregulatory proteins that enable it to switch rapidly between an energy efficient iron-dependent virulent phase and a protective manganese-dependent survival phase.

show abstract

“…(83) The other approach that has been successfully adopted to reduce the sample complexity is the prefractionation of proteins by SDS-PAGE prior to protein digestion and 16 O/ 18 O labeling. (84) The coupling of the SDS-PAGE separation of proteins to the proteolytic 18 O labeling is especially attractive because it overcomes the problem of protein solubilization.…”

Section: Derivative Methodsmentioning

confidence: 99%

Quantitative Proteomics

Chance

Chang

Schlatzer

et al. 2009

Encyclopedia of Analytical Chemistry

View full text Add to dashboard Cite

Quantitative proteomics technologies have been evolving rapidly, but have reached a level of maturity where reliable results of well‐understood statistical significance can be expected from experiments intended to measure changes in protein abundance in paired samples of case versus control or in multiple comparison studies with substantial numbers of biological replicates and multiple treatments. This review provides an overview of the progress and challenges in the field and highlights specific methods to examine changes in protein abundance where the intact protein species are separated and quantified (top‐down proteomics) and where proteins are digested and separated and peptide abundance is quantified (bottom‐up or shotgun proteomics) to infer relative protein abundance. This paper provides specific examples of two‐dimensional (2‐D) gel‐based quantification along with shotgun methods using 18 O‐labeling and label‐free quantification methods. In each case, issues of sample numbers, target variance, sample prefractionation, and other considerations that are informative to the practical use of these techniques are highlighted. The future prospects of this field are bright, but the challenges of integrating the wealth of proteomics data available into an informative biological context remain an area of important future development.

show abstract

Application of ¹⁶O/¹⁸O reverse proteolytic labeling to determine the effect of biofilm culture on the cell envelope proteome of Porphyromonas gingivalis W50

Cited by 50 publications

References 69 publications

Virulence Factors of the Oral Spirochete Treponema denticola

Virulence Factors of the Oral Spirochete Treponema denticola

The interplay between iron, haem and manganese in Porphyromonas gingivalis

Quantitative Proteomics

Contact Info

Product

Resources

About