Application of succulent plant leaves for Agrobacterium infiltration-mediated protein production

F-Box Gene D5RF Is Regulated by Agrobacterium Virulence Protein VirD5 and Essential for Agrobacterium-Mediated Plant Transformation

“…The rosette leaves of 45 day-old plants before flowering were used for RT-PCR. Infiltration was performed as described by Jones [ 58 ] with modification. Agrobacterium strains A281, A281-virD5, EHA105, and its virD5 deletion mutant strain EHA105- vird5 carrying a binary vector pCAMBIA1302 or pCAMBIA1302:VirD5 were activated by inoculating in YEP medium plus 50 mg. ML −1 of Rifampicin and culturing at 28 °C.…”

“…The rosette leaves of 45 day-old plants before flowering were used for RT-PCR. Infiltration was performed as described by Jones [58] with modification. Agrobacterium strains A281, A281-virD5, EHA105, and its virD5 deletion mutant strain EHA105-vird5 carrying a binary vector pCAMBIA1302 or pCAMBIA1302:VirD5 were activated by inoculating in YEP medium plus 50 mg. ML −1 of Rifampicin and culturing at 28 • C. After OD 600 reached 1.0-1.2, the bacteria were collected by centrifugation at 2000× g for 5 min, resuspended to OD 600 of 1.0 with 10 mmol/L MgCl 2 solution, and kept at room temperature for 1 h. The 10 mmol/L MgCl 2 solution was used as Mock.…”

The importance of Rhizobium, Agrobacterium, Bradyrhizobium, Herbaspirillum, Sinorhizobium in sustainable agricultural production

“…ATMT works well with different fungal materials such as spores, mycelia, and gill tissues of mushroom (Chen et al, 2000;Mulllins et al, 2001;Park et al, 2013), which makes it appropriate for fungal genetic manipulation (Xu et al, 2016;Idnurm et al, 2017;Long et al, 2018). In pharmaceutical studies, this technique has been also used to produce various proteins, and general functional studies of plant proteins (O'Neill et al, 2008;Jones, 2016). Several studies introduced ATMT as an initiative bio-transformation system which may provide new insights into fungal pathogenesis, pigmentation, sporulation, and antibiotic resistance (Jeon et al, 2007;Huser et al, 2009;Mischielse et al, 2009;Zhang et al, 2011).…”

Plant vascular system-feeding Psyllidae (Hemiptera) and Nematoda genomes encode family 12 glycosyl hydrolases

“…Plasmid was generated in E. coli, purified, and used to electroporate Agrobacterium tumefaciens Smith and Townsend LB 4404 (Invitrogen). Cells were cultured and used for succulent plant leaf infiltration as previously described (Jones 2016). Samples from infiltrated succulent leaves were tested using azure blue cellulose and xyloglucan at 0.1 mg/mL in water and incubated at 37°C overnight (Jones 2016).…”

“…Cells were cultured and used for succulent plant leaf infiltration as previously described (Jones 2016). Samples from infiltrated succulent leaves were tested using azure blue cellulose and xyloglucan at 0.1 mg/mL in water and incubated at 37°C overnight (Jones 2016). Two infiltration events and extractions were performed.…”