Application of solid-phase microextraction and gas chromatography–mass spectrometry to the determination of volatile organic compounds in end-exhaled breath samples

Prado, C.; Marı́n, Pedro; Periago, J.F.

doi:10.1016/s0021-9673(03)01103-8

Cited by 55 publications

(35 citation statements)

References 24 publications

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“…Glass bulbs can also be used for breath sampling [15,23,28,46,66]. However, they are fragile, require silanization to deactivate the interior glass surface [46,67], and must be evacuated before sampling.…”

Section: Breath-collection Devicesmentioning

confidence: 99%

“…Different procedures are followed in SPME (Table 1) [3,17,35,46,61,66,67,[69][70][71]87,88]. Sometimes the fiber is inserted into the bag or glass bulb containing the total volume of breath collected for a predetermined period of time [46,66,70,71], or a fixed and small volume of the sample is transferred inside a sealed vacuum headspace vial before inserting the SPME fiber into the vial [87,88].…”

Section: Solid-phase Microextraction (Spme)mentioning

confidence: 99%

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Analytical challenges in breath analysis and its application to exposure monitoring

Alonso

Sánchez

2013

TrAC Trends in Analytical Chemistry

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There is an increasing interest in the use of breath analysis for monitoring human physiology and exposure to toxic substances or environmental pollutants.This review focuses on the current status of the sampling procedures, collection devices and sample-enrichment methodologies used for exhaled breath-vapor analysis. We discuss the different parameters affecting each of the above steps, taking into account the requirements for breath analysis in exposure assessments and the need to analyze target compounds at sub-ppbv levels.Finally, we summarize the practical applications of exposure analysis in the past two decades.

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Section: Breath-collection Devicesmentioning

confidence: 99%

Section: Solid-phase Microextraction (Spme)mentioning

confidence: 99%

Analytical challenges in breath analysis and its application to exposure monitoring

Alonso

Sánchez

2013

TrAC Trends in Analytical Chemistry

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“…Even though up to 3000 compounds may be detected in different persons' breath (Phillips et al, 1999a,b), the matrix of exhaled air is less complex than that of blood or other body fluids. This makes breath analysis simpler to perform (Prado et al, 2003;Libardoni et al, 2006). Despite the obvious advantages it presents for routine biological monitoring, it has not yet been introduced as a standard tool into clinical diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Human exhaled air analytics: biomarkers of diseases

Buszewski

Kęsy

Ligor

et al. 2007

Biomedical Chromatography

672

531

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Over the last few years, breath analysis for the routine monitoring of metabolic disorders has attracted a considerable amount of scientific interest, especially since breath sampling is a non-invasive technique, totally painless and agreeable to patients. The investigation of human breath samples with various analytical methods has shown a correlation between the concentration patterns of volatile organic compounds (VOCs) and the occurrence of certain diseases. It has been demonstrated that modern analytical instruments allow the determination of many compounds found in human breath both in normal and anomalous concentrations. The composition of exhaled breath in patients with, for example, lung cancer, inflammatory lung disease, hepatic or renal dysfunction and diabetes contains valuable information. Furthermore, the detection and quantification of oxidative stress, and its monitoring during surgery based on composition of exhaled breath, have made considerable progress. This paper gives an overview of the analytical techniques used for sample collection, preconcentration and analysis of human breath composition. The diagnostic potential of different disease-marking substances in human breath for a selection of diseases and the clinical applications of breath analysis are discussed.

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“…Different methods such as chemical interaction have been developed to analyze volatile metabolites and to compare them in healthy subjects and cancer patients, adsorptive binding, cold trapping and supercritical fluid extraction. The most successful in this field are SPME and the recently developed multi-bed sorption trap [23][24][25]. The SPME technique was developed by Pawliszyn in late 1989 as a new preconcentration technology, in which a fused coated silica fiber is used as the stationary phase [20,26].…”

Section: Introductionmentioning

confidence: 99%

Solid phase microextraction, mass spectrometry and metabolomic approaches for detection of potential urinary cancer biomarkers—A powerful strategy for breast cancer diagnosis

Silva

Passos

Câmara

2012

Talanta

143

119

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a b s t r a c tA sensitive assay to identify volatile organic metabolites (VOMs) as biomarkers that can accurately diagnose the onset of breast cancer using non-invasively collected clinical specimens is ideal for early detection. Therefore the aim of this study was to establish the urinary metabolomic profile of breast cancer patients and healthy individuals (control group) and to explore the VOMs as potential biomarkers in breast cancer diagnosis at early stage. Solid-phase microextraction (SPME) using CAR/PDMS sorbent combined with gas chromatography-mass spectrometry was applied to obtain metabolomic information patterns of 26 breast cancer patients and 21 healthy individuals (controls). A total of seventy-nine VOMs, belonging to distinct chemical classes, were detected and identified in control and breast cancer groups. Ketones and sulfur compounds were the chemical classes with highest contribution for both groups. Results showed that excretion values of 6 VOMs among the total of 79 detected were found to be statistically different (p < 0.05). A significant increase in the peak area of (−)-4-carene, 3-heptanone, 1,2,4-trimethylbenzene, 2-methoxythiophene and phenol, in VOMs of cancer patients relatively to controls was observed. Statiscally significant lower abundances of dimethyl disulfide were found in cancer patients. Bioanalytical data were submitted to multivariate statistics [principal component analysis (PCA)], in order to visualize clusters of cases and to detect the VOMs that are able to differentiate cancer patients from healthy individuals. Very good discrimination within breast cancer and control groups was achieved. Nevertheless, a deep study using a larger number of patients must be carried out to confirm the results.

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Application of solid-phase microextraction and gas chromatography–mass spectrometry to the determination of volatile organic compounds in end-exhaled breath samples

Cited by 55 publications

References 24 publications

Analytical challenges in breath analysis and its application to exposure monitoring

Analytical challenges in breath analysis and its application to exposure monitoring

Human exhaled air analytics: biomarkers of diseases

Solid phase microextraction, mass spectrometry and metabolomic approaches for detection of potential urinary cancer biomarkers—A powerful strategy for breast cancer diagnosis

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