Application of solid matrix priming to ameliorate salinity stress in mung bean (Vigna radiata)

Abstract

Present study aimed to study the responses of mung bean seedlings under 0, 4 and 8dSm -1 concentrations after Solid matrix priming (SMP), using celite matrix 10% moisture with 15 different elicitors such as kinetin, napthalene acetic acid (NAA), abscisic acid (ABA), salicylic acid, chitosan, calcium chloride (CaCl 2 ), gibberellic acid (GA 3 ), putrescine, gallic acid, Catechol, proline, lanthanum chloride (LaCl 3 ) , ethylene glycol bis (2-aminoethyl ether) tetra acetic acid (EGTA), sodium nitroprusside (SNP) and water (hydropriming). The root length (RL), shoot length (SL), RL/SL ratio, average lateral root number, plant fresh weight (FW) and dry weight (DW) were measured after 6 days of germination. Indices like plant height stress tolerance index (PHSI), root length stress tolerance index (RLSI), shoot height stress tolerance index (SHSI), stress tolerance index (STI), root phytotoxicity, shoot phytotoxicity, fresh weight percentage reduction (FWPR), dry weight percentage reduction (DWPR), relative seedling water content (RSWC) were measured. The root length (RL), shoot length (SL), and biomass production along with dry weight percentage reduction (DWPR) were significantly decreased with increasing salt stress. However, priming treatments improved different morphological traits like SL, RL in almost all cases compared to unprimed seeds. Effect of SMP with salicylic acid and chitosan regarding RL and SL under high salinity is noteworthy. In case of FW, only chitosan at 4 dSm -1 and catechol at 8dSm -1 showed significant improvement i.e. 7.46% and 5.74%, respectively, compared to unprimed seeds. The DW at 4 dSm -1 was influenced by GA 3 followed by SNP, in which showed significant enhanced biomass even better than unprimed seedling in control. Overall, among the elicitors studied chitosan followed by salicylic acid showed the best results under salinity stress.

Assessment of Growth Performance and Histochemical Localisation of Reactive Oxygen Species in Fenugreek under Exogenous Calcium Ion Priming

“…The stress tolerance index (STI) of the seedlings was calculated using the following equation (Sen and Mandal, 2018…”

Fe–Mn nanocomposites doped graphene quantum dots alleviate salt stress of Triticum aestivum through osmolyte accumulation and antioxidant defense

“…The efficacy of this prepared nano-conjugate on germination, salinity stress mitigation (NaCl mediated), and seedling growth of bread wheat was compared with the efficiency of simple iron nanoparticles, iron-manganese (Fe–Mn) nanocomposites and normal GQDs. Moreover, synthesized nano-conjugates were applied through solid matrix priming (SMP), the process itself was reported for alleviation of salt stress 24 , 25 . After the application of different nanomaterials, germination-related parameters, stress-related index, phenotypic appearance of seedling, biochemical, enzymatic, and non-enzymatic antioxidant and oxidative stress (both qualitative and quantitative) attributes were examined to evaluate the effectiveness of the applied formulations.…”