Application of Serodiagnostic Test Specific to Strangles in Strangles Outbreak and Genetic Analysis of &lt;i&gt;Streptococcus equi &lt;/i&gt;Isolates

Hobo, Seiji; Muranaka, Masanori; Niwa, Hidekazu; Uchiyama, Takashi; Yoda, Mari; Suzuki, Yumiko; M, Maeda

doi:10.12935/jvma.63.696

Cited by 3 publications

(5 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The seM-LAMP was applied to samples obtained during an outbreak of strangles [6]. The results of the seM-LAMP were identical to those of the culture examination and seM semi-nested PCR of S. equi.…”

Section: Discussionmentioning

confidence: 83%

“…Field samples and preparation of DNA: To investigate the clinical usefulness of the methods, the seM-LAMP and the seM semi-nested PCR were used to screen 590 nasal swabs obtained during an outbreak of strangles [6]. Each nasal swab was suspended in 500 μl normal saline solution, and 50 μl of the suspension solution was used for bacterial extermination.…”

Section: Semi-nested-pcrmentioning

confidence: 99%

“…equi (S. equi) and is a commonly diagnosed and important infectious disease of horses worldwide [2,[6][7][8][13][14][15][16]. It is characterized by a mucopurulent nasal discharge and acute swelling with subsequent abscess formation in the submandibular and retropharyngeal lymph nodes [5,[13][14][15].…”

mentioning

confidence: 99%

“…S. equi infects the horse's cranial lymph glands and is highly communicable to other horses. Outbreaks of strangles have been reported in many countries and can cause scheduled horse racing meets to be cancelled [4,[6][7][8]11]. S. equi produce an M-like protein, SeM, and the seM gene has been proposed as a target gene to distinguish S. equi from different strains of streptococci including Streptococcus equi subsp.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Development and Application of Loop-Mediated Isothermal Amplification Methods Targeting the seM Gene for Detection of Streptococcus equi subsp. equi

Hobo

Niwa

Oku

2012

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

ABSTRACT. Loop-mediated isothermal amplification (LAMP) constitutes a potentially valuable diagnostic tool for rapid diagnosis of contagious diseases. In this study, we developed a novel LAMP method (seM-LAMP) to detect the seM gene of Streptococcus equi subsp. equi (S. equi), the causative agent of strangles in equids. The seM-LAMP successfully amplified the target sequence of the seM gene at 63C within 60 min. The sensitivity of the seM-LAMP was slightly lower than the 2nd reaction of the seM semi-nested PCR. To evaluate the species specificity of the seM-LAMP, we tested 100 S. equi and 189 non-S. equi strains. Significant amplification of the DNA originating from S. equi was observed within 60 min incubation, but no amplification of non-S. equi DNA occurred. The results were identical to those of seM semi-nested PCR. To investigate the clinical usefulness of the methods, the seM-LAMP and the seM semi-nested PCR were used to screen 590 nasal swabs obtained during an outbreak of strangles. Both methods showed that 79 and 511 swabs were S. equi positive and negative, respectively, and the results were identical to those of the culture examination. These results indicate that the seM-LAMP is potentially useful for the reliable routine diagnosis of Streptococcus equi subsp. equi infections.

show abstract

Section: Discussionmentioning

confidence: 83%

Section: Semi-nested-pcrmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Development and Application of Loop-Mediated Isothermal Amplification Methods Targeting the seM Gene for Detection of Streptococcus equi subsp. equi

Hobo

Niwa

Oku

2012

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…S. equi infects the horse’s cranial lymph glands and is highly communicable to other horses. Outbreaks of strangles have been reported in many countries, and sporadic outbreaks have occurred in Japan since 1992 [2, 5, 7, 18]. In recent years, the number of draft horses imported into Japan for a feeder has increased, and accounts for approximately 85% of the total volume of imported horses; strangles has occurred only in herds of draft horses during the quarantine period of importation.…”

mentioning

confidence: 99%

Genetic analysis of Streptococcus equi subsp. equi isolated from horses imported into Japan

Kasuya¹,

Tanaka²,

Oshima³

et al. 2019

J. Vet. Med. Sci.

Self Cite

View full text Add to dashboard Cite

Strangles is a commonly diagnosed and important infectious disease of equids worldwide, caused by Streptococcus equi subsp. equi . We determined the SeM genotypes of S. equi isolated from imported horses at the Japanese border within the past 8 years, which allowed us to classify 12 strains isolated from these horses from each exporter into four allelic groups. These alleles were different from the alleles of past isolates found in Japan. Furthermore, four strains classified into the same allele were isolated from horses from one exporter over several years. In this study, S. equi isolates from different exporters had different SeM alleles. Attention to the hygiene status of farms will be necessary to prevent the incursion of strangles.

show abstract

Survey of Streptococcus equi subsp. equi in Imported Draft Horses and SeM Genetic Analysis of Isolates

Nakajima

Kasuya

Oshima

et al. 2021

J.Jpn.Vet.Med.Assoc.

View full text Add to dashboard Cite

Application of Serodiagnostic Test Specific to Strangles in Strangles Outbreak and Genetic Analysis of <i>Streptococcus equi </i>Isolates

Cited by 3 publications

References 8 publications

Development and Application of Loop-Mediated Isothermal Amplification Methods Targeting the <i>seM</i> Gene for Detection of <i>Streptococcus equi</i> subsp. <i>equi</i>

Development and Application of Loop-Mediated Isothermal Amplification Methods Targeting the <i>seM</i> Gene for Detection of <i>Streptococcus equi</i> subsp. <i>equi</i>

Genetic analysis of <i>Streptococcus equi</i> subsp. <i>equi</i> isolated from horses imported into Japan

Survey of <i>Streptococcus equi</i> subsp. <i>equi</i> in Imported Draft Horses and SeM Genetic Analysis of Isolates

Contact Info

Product

Resources

About