Application of semipermeable membrane device (SPMD) to assess air genotoxicity in an occupational environment

Bonetta, Sara; Carraro, Elisabetta; Bonetta, Silvia; Pignata, Cristina; Pavan, I; Romano, Canzio; Gilli, Giorgio

doi:10.1016/j.chemosphere.2009.02.039

Cited by 17 publications

(10 citation statements)

References 33 publications

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“…Where passive samplers such as PUF or semipermeable membrane devices have been used to monitor PAH in either ambient (Cupr et al, 2006) or indoor and outdoor air in an occupational exposure setting (coke plant) (Bonetta et al, 2009), the highest genotoxic effects are associated with locations where the highest PAH levels have been accumulated by these samplers. Similarly, we previously determined that differences in the potency of AhR activity in indoor vs. outdoor air sampled by PUF passive air samplers reflected potential PAH profiles, although many of the carcinogenic PAHs were not detected (Kennedy et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

“…While relatively few studies (Isidori et al, 2003; Cupr et al, 2006; Slapsyte et al, 2006; Bonetta et al, 2009; Kennedy et al, 2009) have combined passive air sampling with effect based techniques, these samplers potentially provide a more convenient and cost effective means of monitoring the potential for biological effects resulting from exposure to complex mixtures in a range of scenarios. Since the compounds which will be sampled by these PUF samplers are those present predominantly in the vapour phase and in respirable particle size ranges (Chaemfa et al, 2009a), it is likely that effect based estimates derived from these samples will be particularly relevant from an inhalation toxicology perspective.…”

Section: Introductionmentioning

confidence: 99%

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Effect based monitoring of seasonal ambient air exposures in Australia sampled by PUF passive air samplers

Kennedy

Macova

Bartkow

et al. 2010

Atmospheric Pollution Research

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There has been relatively little bioanalytical effect based monitoring conducted using samples derived from polyurethane foam (PUF) passive air samplers. Combining these techniques may provide a more convenient and cost effective means of monitoring the potential for biological effects resulting from exposure to complex mixtures in a range of scenarios. Seasonal polycyclic aromatic hydrocarbon (PAH) levels were monitored at sites around Australia using direct chemical analysis. In addition, both indirect acting genotoxicity (umuC assay) and aryl hydrocarbon receptor (AhR) activity (chemically activated fluorescent gene expression [CAFLUX assay]), which are effects potentially relevant to subsequent carcinogenesis for these compounds, were measured. The levels of PAHs as well as genotoxicity and AhR activity were all higher in winter compared to summer and for sites in urban capital cities compared to other locations. Statistically significant relationships were found between the levels of PAHs and both genotoxicity and AhR activity. The dominant contributors to the total AhR activity, were found to be for compounds which are not resistant to H2SO4/silica gel treatment and were relatively rapidly metabolised that is consistent with a PAH type response. Relative potency estimates for individual PAHs determined for the first time on the CAFLUX assay were used to estimate the proportion of total AhR activity (≤ 3.0%) accounted by PAHs monitored. Observed responses are thus largely due to non-quantified AhR active compounds.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect based monitoring of seasonal ambient air exposures in Australia sampled by PUF passive air samplers

Kennedy

Macova

Bartkow

et al. 2010

Atmospheric Pollution Research

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“…Effect-based monitoring of exposures sampled by passive air samplers has been utilised previously in ambient air for monitoring mutagenicity, genotoxicity, AhR activity and cytotoxicity [17–22]. These studies have typically expressed the potency of effect on a per sampler basis.…”

Section: Introductionmentioning

confidence: 99%

Assessing indoor air exposures using passive sampling with bioanalytical methods for estrogenicity and aryl hydrocarbon receptor activity

et al. 2009

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Passive air sampling was undertaken using polyurethane foam passive air samplers at three types of locations, including indoors (six offices) at buildings in the central business district (CBD) and at a private suburban home (indoor and outdoor) located 9 km from the CBD in Brisbane, Queensland, Australia. Estrogenic (E-SCREEN—MCF7-BOS) and aryl hydrocarbon receptor (AhR) (CAFLUX— H4G1.1c2) activity were assessed for samples collected from each of these locations. The samples were tested either as crude extracts (“untreated”) or were subjected to H2SO4 silica gel (“treated”) for each location in order to determine whether chemicals, which are not resistant to this treatment like polycyclic aromatic hydrocarbons, potentially account for the observed activity. In most cases, H2SO4 treatment resulted in a statistically significant reduction of potency for both endpoints, suggesting that chemicals less resistant to treatment may be responsible for much of the detected biological activity in these locations. Estrogenic potency measurements (<0.22–185 pg m−3) were highest in the indoor offices, followed by the indoor suburban home and finally the outdoor suburban home (which was not estrogenic). Total AhR activity for crude extracts (1.3–10 pg m−3) however was highest for the outdoor suburban home site. Levels of polycyclic aromatic hydrocarbons were monitored indoors and outdoors at the suburban home. At that location, polycyclic aromatic hydrocarbon air concentrations were on average approximately two times higher outdoor than indoor, while AhR potency was five times higher outdoor than indoor. No significant correlation was found between the estrogenic and AhR activity (P=0.88) for the sites in this study.

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“…Today, the techniques used to monitor environmental pollutants should enable direct monitoring of the fate and concentration of chemical pollutants as well as evaluation of their effects and the potential hazard for human health. Estimation of human exposure to toxic and genotoxic environmental pollutants is a fundamental aspect of human hazard assessment [3,4,5].…”

Section: Introductionmentioning

confidence: 99%

Laboratory calibration of twelve pesticides using polar organic chemical integrative samplers

Thomatou

Αντωνοπούλου

Michail

et al. 2015

International Journal of Environmental Analytical Chemistry

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Application of semipermeable membrane device (SPMD) to assess air genotoxicity in an occupational environment

Cited by 17 publications

References 33 publications

Effect based monitoring of seasonal ambient air exposures in Australia sampled by PUF passive air samplers

Effect based monitoring of seasonal ambient air exposures in Australia sampled by PUF passive air samplers

Assessing indoor air exposures using passive sampling with bioanalytical methods for estrogenicity and aryl hydrocarbon receptor activity

Laboratory calibration of twelve pesticides using polar organic chemical integrative samplers

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