Application of selection index for rice mutant screening under a drought stress condition imposed at reproductive growth phase

SARWENDAH, MAMIK; LUBIS, ISKANDAR; JUNAEDI, AHMAD; Purwoko, Bambang Sapta; Sopandie, Didy; DEWI, AZRI KUSUMA

doi:10.13057/biodiv/d231056

Cited by 2 publications

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References 24 publications

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“…This phenomenon leads to drought stress against plants which is a problem for agriculture around the world. Global warming affects crop production (Sharma et al 2022) and reduces rice supplies (Moonmoon and Islam 2017;Sarwendah et al 2022). This is a challenge in the future, considering that rice is the most vulnerable crop to climate change (Mohanty et al 2013) Drought stress reduces rice production by inhibiting cell growth, cell proliferation, and differentiation, causing the accumulation of reactive oxygen species (Upadhyaya and Panda 2019;Liu et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Polymorphism analysis of drought tolerance gene OsDREB2A in Indonesian local rice from Lampung, Indonesia

et al. 2022

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Abstract. Chrisnawati L, Ernawiati E, Yulianty, Hariri MR. 2022. Polymorphism analysis of drought tolerance gene OsDREB2A in Indonesian local rice from Lampung, Indonesia. Biodiversitas 23: 6352-6357. Drought stress is a significant threat to rice cultivation and the selection of drought-tolerant rice plants is needed to find superior plants. The OsDREB2A gene plays a role in the regulation of drought tolerance and is widely used as a marker. Therefore, this study aims to analyze the OsDREB2A gene polymorphism in the local rice variety of Lampung Lumbung Sewu Cantik, compared to the Inpago 8 drought-tolerant and the IR64 sensitive variety. DNA extraction was performed on fresh leaves using the GENEAID Genomic DNA Mini Kit, amplification by PCR was carried out with the OsDREB2A primer pair, while sequence analysis was conducted with MEGA X and confirmed using the BLAST program. The sequencing results showed that the three rice varieties had the same sequence length of 250 bp. The Lumbung Sewu Cantik variety has the same sequence as Inpago 8, while IR64 has an insertion that causes a frameshift mutation. Furthermore, phylogenetic analysis showed that the Lumbung Sewu Cantik variety was in a similar group with tolerant varieties, while IR64 was in a different group. The similarity of the OsDREB2A Lumbung Sewu Cantik sequence is presumably associated with tolerance to drought.

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Section: Introductionmentioning

confidence: 99%

Polymorphism analysis of drought tolerance gene OsDREB2A in Indonesian local rice from Lampung, Indonesia

et al. 2022

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The Integrated Minapadi (Rice-Fish) Farming System: Compost and Local Liquid Organic Fertilizer Based on Multiple Evaluation Criteria

et al. 2023

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Rice-fish cultivation, also known as Manipadi in Indonesia, is one of the many integrated farming systems where fish are grown concurrently with rice. However, this integrated system needs to be optimized by organic fertilizer. The application of organic fertilizers, such as compost and local biofertilizers, when implemented into this integrated system, can optimize the yield per land for both rice and fish. However, this combination must be evaluated systematically by multiple evaluation criteria and statistical analyses. Therefore, this study aimed to identify the combination of compost doses and/or local liquid organic fertilizer concentrations, based on multiple evaluation criteria, that supports the productivity of rice-fish integration agriculture. The experiment was carried out in the Wanuae farmer group of the Barebbo District, Bone Regency, Indonesia, between September 2018 and January 2019. The experiment employed a split plot design with a randomized complete block design (RCBD). The main plot consisted of compost (four levels), and the subplot was the local biofertilizer source (four levels). The results demonstrated that the number of fill grains was significantly affected by the compost treatment and the type of local biofiller. The effect of the compost dosing was more dominant in comparison with the local liquid organic fertilizer character and its interactions. A compost dosage of four tons ha−1 and a local liquid organic fertilizer, sourced from soaked coconut fiber with cow urine, has been recommended as the combination treatment to optimize the rice and fish yield potential. The Minapadi (rice fish) with additional high composting effectively increased the farmer’s outcome with a 2-point R/C ratio, more so than those without composting. Moreover, the compost dosage in this Minapadi study requires further exploration for a better understanding.

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Application of selection index for rice mutant screening under a drought stress condition imposed at reproductive growth phase

Cited by 2 publications

References 24 publications

Polymorphism analysis of drought tolerance gene OsDREB2A in Indonesian local rice from Lampung, Indonesia

Polymorphism analysis of drought tolerance gene OsDREB2A in Indonesian local rice from Lampung, Indonesia

The Integrated Minapadi (Rice-Fish) Farming System: Compost and Local Liquid Organic Fertilizer Based on Multiple Evaluation Criteria

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