Application of selected biological control agents in conjunction with tolclofos-methyl for the control of damping-off caused by Rhizoctonia solani

Abstract

Selected Trichoderma and Bacillus isolates were tested as seed treatments with reduced concentrations of tolclofos-methyl against Rhizoctonia solani damping-off using cucumber as test plants. In vitro bioassays using filter paper disc infused with six concentrations [active ingredient (ai)] of tolclofos-methyl (0.005, 0.05, 0.125, 0.25, 0.5 and 1.5 g ai/l) showed that growth of Trichoderma isolates on agar plates were less inhibited at 0.005 and 0.05 g ai/l than 0.125-1.5 g ai/l tolclofos-methyl concentrations while Bacillus isolates were not affected by any of the six concentrations. In a greenhouse study with a 0.05 g ai/l tolclofos-methyl concentration, Trichoderma and Bacillus isolates applied alone or in combination to cucumber seeds resulted in a better disease control than the Trichoderma and Bacillus treatments used alone. As high as 86% disease control was achieved by combining 0.05 g ai/l tolclofos-methyl with T. harzianum Isolate SYN which was significantly better (P = 0.001) than the 0.05 g ai/l tolclofos-methyl control. In all cases, additive effects of combining 0.05 g ai/l tolclofos-methyl with Trichoderma and Bacillus treatments were observed.

Activity Interactions of Crude Biopreservatives against Spoilage Yeast Consortia

“…a b In a review by Kumar and Jagadeesh [23], several microbial consortia against phytopathogens were highlighted. The microbial consortia reported thus far against Botrytis, Colletotrichum, Rhizoctoniasolani, and Pyriculariaoryzae species were composed of two or more species of yeasts and/or bacteria acting on wounded fruits [23][24][25][26][27].…”

Untitled

“…Ofrece una alternativa a los productos químicos, contribuyendo a minimizar las consecuencias negativas para la salud humana y el medio ambiente (O'Brien, 2017). Los agentes del control biológico son frecuentemente probados, desarrollados y usados como un esfuerzo para el control de varios fitopatógenos del suelo (Yobo et al, 2010). Las bacterias endofíticas pueden definirse como aquellas que se pueden aislar de tejidos vegetales sanos y superficialmente desinfectados sin causar ningún daño a la planta huésped (Compant et al, 2005;Miliute et al, 2015); son capaces de penetrar y diseminarse sistémicamente en la planta huésped, colonizando activamente los vasos conductores, y ocasionalmente los espacios intracelulares.…”

“…Biological control agents are frequently tested, developed and used as an effort to control several soil borne plant pathogens (Yobo et al, 2010). Endophytic bacteria can be defined as those that can be isolated from healthy plant tissues and superficially disinfected without causing any damage to the host plant (Compant et al, 2005;Miliute et al, 2015); they are capable of penetrating and disseminating systemically in the host plant, actively colonizing the conductive vessels, and occasionally the intracellular spaces.…”