Application of secondary ion and matrix‐assisted laser desorption‐ionization time‐of‐flight mass spectrometry for the quantitative analysis of biological molecules

Muddiman, David C.; Gusev, Arkady I.; Hercules, David M.

doi:10.1002/mas.1280140603

Cited by 52 publications

(51 citation statements)

References 161 publications

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“…Although proteins with characteristics unlike those of the analyte may be used as internal standards (as has been shown during protein quantification directly from mixtures (18,19) or during MALDI-TOF quantification of affinity-retrieved species by addition of an internal reference standard to peptides eluted from beaded affinity reagent (20)), internal reference standards that behave similarly to the analyte during laser desorption/ionization are generally preferred. This prerequisite is met during MSIA by choosing internal references that share sequence homology with the target protein: enzymatic/chemically modified versions of the targeted protein (15,16,21), truncated/ extended recombinant forms of the target proteins, the (same) target protein recombinantly expressed in isotopically enriched medium (e.g., 15 N or 18 O), or the same protein from a different biological species.…”

Section: Quantificationmentioning

confidence: 99%

Detection and Quantification of β-2-Microglobulin Using Mass Spectrometric Immunoassay

Tubbs

Nedelkov

Nelson

2001

Analytical Biochemistry

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Section: Quantificationmentioning

confidence: 99%

Detection and Quantification of β-2-Microglobulin Using Mass Spectrometric Immunoassay

Tubbs

Nedelkov

Nelson

2001

Analytical Biochemistry

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“…Extensive and well-documented work 13,14 has been done to improve drug quantitation by MALDI-MS since its capabilities were initially reported more than a decade ago, [15][16][17] bringing about the first commercially available MALDI system dedicated to quantitation, FlashQuant (AB/ MDS Sciex). 18 The original matrix-free laser desorption/ ionization (matrix-free LDI) 19 procedure avoids the need to add matrix and eliminates the strong matrix interference in the low molecular weight range present in MALDI-MS; however, the complexity and diversity of the material required in the newer implementations of this method of analysis (e.g.…”

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confidence: 99%

Quantitative analysis of small molecules by desorption electrospray ionization mass spectrometry from polytetrafluoroethylene surfaces

Ifa¹,

Manicke²,

Rusine³

et al. 2008

Rapid Comm Mass Spectrometry

122

109

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Desorption electrospray ionization mass spectrometry (DESI-MS) is an emerging technique for ambient analysis. However, its application to routine quantitation has not been explored extensively and this is undertaken here. We present studies that utilize a particularly suitable surface, porous polytetrafluoroethylene (porous PTFE), which shows less cross contamination between samples and improved sensitivity and signal stability compared to other surfaces. Quantitative experiments for 1 microL spotted solutions of the beta-blocker propranolol, using isotopically labeled propranolol as internal standard, showed a good linear correlation (r2 > 0.996) over the range 0.01-100 microM. The inter-day precision, based on the relative standard deviation, for replicates analyzed on three different days was 13% for 0.01 microM and better than 7% for the remainder of the calibration points. The inter-day accuracy, expressed as relative error, was better than +/-7% for all calibration points along the curve. These day-to-day measurements suggest that DESI-MS can be successfully employed for routine quantitative analysis. The use of the analog atenolol as an internal standard and further considerations that should improve quantitation by DESI-MS are also presented.

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“…Recently, MALDI-TOF-MS has also been developed to analyze small molecules [13] successfully by using the different matrix substances, such as desorption/ionization on porous silicon (DIOS) [14 -17], matrix with high molecular weight [18,19], surfactant suppressed matrix [20], inorganic materials [21][22][23][24][25], etc. Though considerable works of quantitative determination using MALDI have been well documented and reviewed [26], quantitative analysis by MALDI is still considered as a relatively unreliable technique for the limiting factor of reproducibility within and between sample spots. Recently, Siuzdak and coworkers [27] developed a method for quantitative analysis of small molecules with desorption/ionization on porous silicon using electrospray deposition of analytes, and a quite good quantitative result with linear calibrations R 2 Ͼ 0.99 and values of RSD Ͻ 7% was achieved.…”

mentioning

confidence: 99%

Using oxidized carbon nanotubes as matrix for analysis of small molecules by MALDI-TOF MS

Pan

et al. 2005

J. Am. Soc. Mass Spectrom.

154

147

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Oxidized carbon nanotubes are tested as a matrix for analysis of small molecules by matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). Compared with nonoxidized carbon nanotubes, oxidized carbon nanotubes facilitate sample preparation because of their higher solubility in water. The matrix layer of oxidized carbon nanotubes is much more homogeneous and compact than that of nonoxidized carbon nanotubes. The efficiency of desorption/ionization for analytes and the reproducibility of peak intensities within and between sample spots are greatly enhanced on the surface of oxidized carbon nanotubes. The advantage of the oxidized carbon nanotubes in comparison with ␣-cyano-4-hydroxycinnamic acid (CCA) and carbon nanotubes is demonstrated by MALDI-TOF-MS analysis of an amino acid mixture. The matrix is successfully used for analysis of synthetic hydroxypropyl ␤-cyclodextrin, suggesting a great potential for monitoring reactions and for product quality control. Reliable quantitative analysis of jatrorrhizine and palmatine with a wide linear range (1-100 ng/mL) and good reproducibility of relative peak areas (RSD less than 10%) is achieved using this matrix. Concentrations of jatrorrhizine (8.65 mg/mL) and palmatine (10.4 mg/mL) in an extract of Coptis chinensis Franch are determined simultaneously using the matrix and a standard addition method. [27] developed a method for quantitative analysis of small molecules with desorption/ionization on porous silicon using electrospray deposition of analytes, and a quite good quantitative result with linear calibrations R 2 Ͼ 0.99 and values of RSD Ͻ 7% was achieved. It is believed that MALDI could be a powerful technique to provide both qualitative and quantitative determination of small compounds.Carbon nanotubes have been attracting wide interest [28,29] of scientists since they were initially discovered by Iijima et al. [30,31]. Recently, Carbon nanotubes have been developed as the matrix for MALDI-TOF-MS for analysis of small molecules [25], in which carbon nanotubes function both as the energy receptacle for laser radiation and as the energy transporter for desorption/ionization of analytes with the minimization of interference signals caused by matrix ion. The effectiveness of the method as matrix has been demonstrated by several compounds with low molecular weight. However, the low solubility of carbon nanotubes in water or organic solvent makes it hard to deposit carbon nanotubes onto the sample target and to form a homogeneous layer of matrix, leading to the relatively poor reproducibility and resolution of peaks for analytes.

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Application of secondary ion and matrix‐assisted laser desorption‐ionization time‐of‐flight mass spectrometry for the quantitative analysis of biological molecules

Cited by 52 publications

References 161 publications

Detection and Quantification of β-2-Microglobulin Using Mass Spectrometric Immunoassay

Detection and Quantification of β-2-Microglobulin Using Mass Spectrometric Immunoassay

Quantitative analysis of small molecules by desorption electrospray ionization mass spectrometry from polytetrafluoroethylene surfaces

Using oxidized carbon nanotubes as matrix for analysis of small molecules by MALDI-TOF MS

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