Application of <scp>L</scp>‐(+)‐Lactate electrode for clinical analysis and monitoring of tissue culture medium

Tsuchida, Toshio; Takasugi, Hiroshi; Yoda, Kentaro; Takizawa, Kouichi; Kobayashi, Sohei

doi:10.1002/bit.260270613

Cited by 26 publications

(8 citation statements)

References 10 publications

(1 reference statement)

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“…3 for the latter. The pH optimum of soluble LOD has been reported to be around 7 [27,28]. In contrast, the pH to obtain maximal fluorescence signal intensity of the bienzyme optode was even higher than that of the HRP sensor, i.e.…”

Section: Lactate Sensormentioning

confidence: 91%

Fibre optic fluorometric enzyme sensors for hydrogen peroxide and lactate, based on horseradish peroxidase and lactate oxidase

1995

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Abstract. An optical biosensor for the determination of hydrogen peroxide based on immobilized horseradish peroxidase is described. The fluorescence of the dimeric product of the enzyme catalysed oxidation of homovanillic acid is utilized to determine the concentration of H20 2. The membrane-bound enzyme is attached to a bifurcated fibre bundle permitting excitation and detection of the fluorescence by a fluorometer. The response of the sensor is linear from 1 to 130 gM hydrogen peroxide; the coefficient of variation is 3 %. The sensor is stable for more than 10 weeks. The operating pH for maximal sensor response is 8.15. This allows the sensor to be used in combination with oxidase reactions producing hydrogen peroxide, as is demonstrated with a co-immobilized lactate oxidase-horseradish peroxidase optode for the determination of L-lactate. The fluorescence intensity of this sensor depends linearly on the concentration of lactate between 3 and 200 pM and a throughput of 10 samples per hour is possible. The precision is in the same range as that of the monoenzyme optode. The lifetime of the bienzyme sensor for lactate is considerably shorter than that of the peroxidase sensor; it is limited by the stability of the immobilized lactate oxidase enzyme. The sensor has been applied to the determination of lactate in control serum.Key words: optical biosensor, enzyme optode, hydrogen peroxide, horseradish peroxidase, lactate determination, lactate oxidase.Within the field of biomolecular sensor research, recent years have seen considerable effort to develop optical biosensors (opt[r]odes) by coupling biorecognition elements to fibre optic waveguides (see [1] for review). Fibre optic biosensors offer distinct advantages, such as minute size, no need for reference, no electrical contacts in the * Present address:

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Section: Lactate Sensormentioning

confidence: 91%

Fibre optic fluorometric enzyme sensors for hydrogen peroxide and lactate, based on horseradish peroxidase and lactate oxidase

1995

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“…In the HER-100 (Omron Tateisi, Japan) an asymmetric cellulose acetate membrane is used, bearing LOD covalently bound by y-aminopropyltriethoxysilane and cross-linked with glutaraldehyde [371]. The membrane is highly selective for hydrogen peroxide.…”

Section: (14-23)mentioning

confidence: 99%

“…Process control in human and animal cell cultures is very important because the required nutrients, eg, fetal calf serum, are extremly expensive [416]. For optimization of cell culture, the HER-100 lactate analyzer (Omron Tateisi, Japan) has been used together with a glucose electrode for lactate and glucose analysis in the growth medium of human melanoma cells [417]. Investigation of the process over 7 d showed that, as a result of glycolysis, with increasing cell number the concentration of glucose decreased and that .of lactate increased.…”

Section: Food Analysis Process Control and Environmental Monitoringmentioning

confidence: 99%

Specific Features of Biosensors

Schmidt¹,

Schuhmann²,

Mnchen³

et al. 1995

Sensors Set

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“…The great advantage of immobilized enzymes is that they have very high stabilities as compared with native enzymes. Many techniques of immobilization are available for industrial appli-cations using different matrices [11][12][13][14][15]. Rothe and Aurich [16] proposed particles covalently coated with a phospholipid bilayer coated in organic colloids.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of cholesterol oxidase on Formvar using organic solvents

Kumar,

Kumari

et al. 1999

Biotech and App Biochem

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The present study describes a new immobilization support for the preparation of enzyme membrane in the presence of organic solvents. The support was composed of Formvar solubilization in organic solvents with enzyme. More than 90% of the enzyme was immobilized on the membrane. The membrane was prepared by mixing 4% Formvar in organic solvents and 1% cholesterol oxidase was immobilized by entrapment technique. Practically no leaching of the entrapped enzyme was observed. The resultant immobilized enzyme membrane was stored at 4 °C for 10 days without losing its activity. The pH and temperature stabilities were greater than those of the native enzyme. The immobilized enzyme membrane has a long life due to its hydrophobic nature, as compared with other membranes.

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Application of L‐(+)‐Lactate electrode for clinical analysis and monitoring of tissue culture medium

Cited by 26 publications

References 10 publications

Fibre optic fluorometric enzyme sensors for hydrogen peroxide and lactate, based on horseradish peroxidase and lactate oxidase

Fibre optic fluorometric enzyme sensors for hydrogen peroxide and lactate, based on horseradish peroxidase and lactate oxidase

Specific Features of Biosensors

Immobilization of cholesterol oxidase on Formvar using organic solvents

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