Application of scanning UV microspectrophotometry for the topochemical detection of lignin within individual cell walls of brown-rotted Scots pine (Pinus sylvestris L.) sapwood

Irbe, Ilze; Noldt, Guna; Koch, Gerald; Andersone, Ingeborga; Andersons, Bruno

doi:10.1515/hf.2006.102

Cited by 15 publications

(12 citation statements)

References 21 publications

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“…UV spectrophotometry provides another analytical tool for lignin structural analysis, as lignin absorbs light in this region of the electromagnetic spectrum [44,128,129,133,[173][174][175].Phenolic hydroxyl groups were quantified using differential UV spectrophotometry by evaluating technical lignins using wavelengths at 300 and 360 nm [44]. The change in molar absorptivity (Δε) of the unknowns was compared with that of model compounds.…”

Section: Electronic Spectroscopymentioning

confidence: 99%

“…The band at 315 nm decreased first, indicative of the removal of hydroxycinnamic acids in the beginning stages of delignification. Lignin detection in the cell walls of brown-rotted Scots pine was evaluated using scanning UV microspectrophotometry [173]. Sapwood samples were incubated for 6, 8, 10, 30 and 50 days with the fungus Coniophora puteana.…”

Section: Electronic Spectroscopymentioning

confidence: 99%

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Recent innovations in analytical methods for the qualitative and quantitative assessment of lignin

Lupoi

Singh

Parthasarathi

et al. 2015

Renewable and Sustainable Energy Reviews

296

188

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a b s t r a c tAs the attraction of creating biofuels and bio-based chemicals from lignocellulosic biomass has increased, researchers have been challenged with developing a better understanding of lignin structure, quantity and potential uses. Lignin has frequently been considered a waste-product from the deconstruction of plant cell walls, in attempts to isolate polysaccharides that can be hydrolyzed and fermented into fuel or other valuable commodities. In order to develop useful applications for lignin, accurate analytical instrumentation and methodologies are required to qualitatively and quantitatively assess, for example, what the structure of lignin looks like or how much lignin comprises a specific feedstock's cellular composition. During the past decade, various diverse strategies have been employed to elucidate the structure and composition of lignin. These techniques include using two-dimensional nuclear magnetic resonance to resolve overlapping spectral data, measuring biomass with vibrational spectroscopy to enable modeling of lignin content or monomeric ratios, methods to probe and quantify the linkages between lignin and polysaccharides, or refinements of established methods to provide higher throughput analyses, less use of consumables, etc. This review seeks to provide a comprehensive overview of many of the advancements achieved in evaluating key lignin attributes. Emphasis is placed on research endeavored in the last decade.

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Section: Electronic Spectroscopymentioning

confidence: 99%

Section: Electronic Spectroscopymentioning

confidence: 99%

Recent innovations in analytical methods for the qualitative and quantitative assessment of lignin

Lupoi

Singh

Parthasarathi

et al. 2015

Renewable and Sustainable Energy Reviews

296

188

View full text Add to dashboard Cite

show abstract

“…Accordingly, cellular UV-microspectrophotometry (UMSP) was applied to characterize the delignification process of P. vitreus on Norway spruce after incubation times of up to 9 weeks. The UMSP technique is well suitable for detecting the topochemical distribution of lignin at a subcellular level (Koch and Kleist 2001;Irbe et al 2006;Kim et al 2008;Lehringer et al 2008;Prislan et al 2009). Bauch et al (1976) applied UMSP successfully to investigate topochemical delignification caused by fungal decay in woody tissue.…”

Section: Introductionmentioning

confidence: 99%

Effect of Physisporinus vitreus on wood properties of Norway spruce. Part 1: Aspects of delignification and surface hardness

et al. 2011

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The white rot fungus Physisporinus vitreus is currently tested for several biotechnological applications such as permeability improvement of refractory wood species or the optimization of the acoustic properties of wood for violins. The enzymatic activity of P. vitreus results in the degradation of pit membranes and simultaneous alterations of the tracheid cell wall structure in wood of Norway spruce wPicea abies (L.) Karstx. By this means, selective delignification and simultaneous degradation occur in the latewood tracheids at short incubation times. To study the delignification of individual cell wall layers in latewood tracheids, cellular UVmicrospectrophotometry was applied to wood of Norway spruce that had been incubated for between 3 and 9 weeks. By means of this technique, the progressing delignification was demonstrated in the latewood tracheid secondary walls. Moreover, local delignification in close proximity to hyphal tunneling, cavities, and notches was evident. Additionally, the mechanical changes were measured (a) at the macroscopic level by Brinell hardness test and (b) at the cellular level by nanoindentation. Brinell hardness was significantly reduced with increasing incubation time which was attributed to the partial delignification. Unlike Brinell tests, results from nanoindentation tests did not show a clear effect of fungal activity because of the material heterogeneity and the high spatial resolution of this technique. The present study provides methodological approaches for the investigation of wood-fungus interactions and contributes to a better understanding of the characteristics of wood decay at the subcellular level caused by the white rot fungus P. vitreus. Moreover, it establishes the basis for a subsequent chemical analysis, for which the results will be the topic of a second paper in this series.

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“…In addition, UV absorbance profiles at constant wavelengths were generated in the scanning mode of the microspectrophotometer using the scan program APAMOS (Zeiss). The scan program creates absorbance profiles at constant wavelengths with a spatial resolution of 0.25μm 2 and a photometrical resolution of 4096 grey scales, which are converted into 14 basic colours visualising the intensity of absorbance (Irbe et al 2006).…”

Section: Ultraviolet (Uv) Microspectrophotometrymentioning

confidence: 99%

Chemical composition and natural durability of juvenile and mature heartwood of Robinia pseudoacacia L.

Latorraca

Dünisch²,

Koch

2011

An. Acad. Bras. Ciênc.

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The aim of this study was to characterize the properties of juvenile and mature heartwood of Robinia pseudoacacia L. (black locust). The content, the composition, and subcellular localization of heartwood extractives were studied in 14 old-grown trees from forest sites in Germany and Hungary, as well as in 16 younger trees of four clone types. Heartwood extractives (methanol and acetone extraction) were analysed by HPLC-chromatography. UV microspectrophotometry was used to localize the extractives in the wood cell walls. The natural durability of juvenile and mature heartwood was analysed according to the European standard EN 350-1. Growth analyses, as well as the chemical analyses, showed that in Robinia the formation of juvenile wood is restricted to the first 10-15 years of cambial growth. In the heartwood high contents of phenolic compounds and flavonoids were present, which were in high concentrations in the cell walls of the axial parenchyma and of the vessels. In the juvenile heartwood, the content of these extractives is significantly lower than in the mature heartwood. In agree, the juvenile heartwood had a lower resistance to decay by Coniophora puteana (brown rot fungus) and Coriolus versicolor (white rot fungus) compared to the mature.

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Application of scanning UV microspectrophotometry for the topochemical detection of lignin within individual cell walls of brown-rotted Scots pine (Pinus sylvestris L.) sapwood

Cited by 15 publications

References 21 publications

Recent innovations in analytical methods for the qualitative and quantitative assessment of lignin

Recent innovations in analytical methods for the qualitative and quantitative assessment of lignin

Effect of Physisporinus vitreus on wood properties of Norway spruce. Part 1: Aspects of delignification and surface hardness

Chemical composition and natural durability of juvenile and mature heartwood of Robinia pseudoacacia L.

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